Single-cell suspensions of whole moue lung were prepared by mincing through a 40-μm Falcon cell strainer, followed by RBC lysis (ACK lysis buffer) for 3 minutes. Lung CD45+ cells were labeled using microbeads-conjugated to monoclonal anti-mouse CD45 (130-052-301, Miltenyi Biotec) and isolated using autoMACS separator. Sorted cells (0.3 × 106 cells per well) were cultured in the presence of anti-IL-17a (30 μg/ml) or isotype control (30 μg/ml) at 37°C for 24 hr. T-bet and Rorγt expression in γδ and CD4 T cells were quantified using fluorescently labeling (PE/Cy7-T-bet or PE-Rorγt).
Anti mouse cd45
Manufactured by Miltenyi Biotec
Anti-mouse CD45 is a laboratory product used for the detection and identification of mouse CD45 positive cells. It is a monoclonal antibody that binds specifically to the CD45 antigen expressed on the surface of mouse immune cells. This product can be used in various cell analysis techniques, such as flow cytometry, to study the phenotype and function of mouse leukocytes.
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Lab products found in correlation
2 protocols using anti mouse cd45
1
Isolation and Analysis of Lung Immune Cells
2
Isolation and Analysis of Lung Immune Cells
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Single-cell suspensions of whole moue lung were prepared by mincing through a 40-μm Falcon cell strainer, followed by RBC lysis (ACK lysis buffer) for 3 minutes. Lung CD45+ cells were labeled using microbeads-conjugated to monoclonal anti-mouse CD45 (130-052-301, Miltenyi Biotec) and isolated using autoMACS separator. Sorted cells (0.3 × 106 cells per well) were cultured in the presence of anti-IL-17a (30 μg/ml) or isotype control (30 μg/ml) at 37°C for 24 hr. T-bet and Rorγt expression in γδ and CD4 T cells were quantified using fluorescently labeling (PE/Cy7-T-bet or PE-Rorγt).
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