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Lipofectamine 2000 reagent

Manufactured by Qiagen
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Lipofectamine 2000 reagent is a transfection reagent used for the delivery of nucleic acids, such as DNA or RNA, into mammalian cells. It is a cationic lipid-based reagent that forms complexes with the nucleic acids, which can then be taken up by the cells.

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Lab products found in correlation

Nlrp1 sirna, by Qiagen (2 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) Rpmi 1640, by Thermo Fisher Scientific (2 mentions) Penicillin, by Solarbio (2 mentions) Streptomycin, by Solarbio (2 mentions) Si xist, by GenePharma (1 mentions) Scrambled sirna control si nc, by GenePharma (1 mentions) Pcdna, by GenePharma (1 mentions)

5 protocols using lipofectamine 2000 reagent

1

NLRP1 Silencing in Cells

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Cells were transfected with 40 nM NLRP1 siRNA (a mixture of two preselected siRNAs for NLRP1) or negative control (Qiagen Valencia, CA) using Lipofectamine 2000 reagent in OPTI-MEM1 reduced serum medium overnight.
Zhai Z., Liu W., Kaur M., Luo Y., Domenico J., Samson J.M., Shellman Y.G., Norris D.A., Dinarello C.A., Spritz R.A, & Fujita M. (2017). NLRP1 promotes tumor growth by enhancing inflammasome activation and suppressing apoptosis in metastatic melanoma. Oncogene, 36(27), 3820-3830.
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2

Modulating XIST and PAPPA in HUVECs

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Small interfering RNA (siRNA) against XIST (si-XIST) or PAPPA (si-PAPPA) and siRNA scrambled control (si-NC), XIST overexpression vector (pcDNA-XIST), and its negative control (pcDNA) were acquired from GenePharma (Shanghai, China). MiR-98-5p mimic (miR-98-5p) and control (miR-NC) and miR-98-5p inhibitor (anti-miR-98-5p) and control (anti-NC) were provided by Sangon (Shanghai, China). Different oligonucleotides or vectors were transfected into HUVECs with Lipofectamine 2000 reagent (Qiagen) following the user’s guidebook.
Gao H, & Guo Z. (2021). LncRNA XIST regulates atherosclerosis progression in ox-LDL-induced HUVECs. Open Medicine, 16(1), 117-127.
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3

NLRP1 Silencing in Cells

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Cells were transfected with 40 nM NLRP1 siRNA (a mixture of two preselected siRNAs for NLRP1) or negative control (Qiagen Valencia, CA) using Lipofectamine 2000 reagent in OPTI-MEM1 reduced serum medium overnight.
Zhai Z., Liu W., Kaur M., Luo Y., Domenico J., Samson J.M., Shellman Y.G., Norris D.A., Dinarello C.A., Spritz R.A, & Fujita M. (2017). NLRP1 promotes tumor growth by enhancing inflammasome activation and suppressing apoptosis in metastatic melanoma. Oncogene, 36(27), 3820-3830.
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4

ESCC Cell Line Cultivation and Transfection

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ESCC cell lines Ec109 was obtained from the institute of Basic Medical Sciences, Chinese Academy of Medical Sciences. ESCC cell line Ec9706 was obtained from Fuxiang biotechnology of Shanghai (Shanghai, China). Cells were maintained in Dulbecco's modi ed Eagle's medium (DMEM) or RPMI 1640 (Gibco) supplemented with 10% fetal bovine serum (Gibco), 100 units of penicillin/ml (Solarbio) and 100 mg of streptomycin/ml (Solarbio) in humidi ed air at 37°C with 5% CO2. HPV16 E6 plasmid and negative control (HPV16 E6 empty vector plasmid) were designed and synthesized by Heyuan company of Shanghai. Cell transfections were performed with Lipofectamine 2000 reagent (QIAGEN company) by following manufacturers' instructions.
Hu J., Ji Y., Miao T., Zheng S., Cui X., Hu J., Yang L, & Li F. (2023). HPV 16 E6 promotes growth and metastasis of esophageal squamous cell carcinoma cells in vitro. Molecular biology reports, 50(2).
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5

ESCC Cell Line Cultivation and Transfection

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ESCC cell lines Ec109 was obtained from the institute of Basic Medical Sciences, Chinese Academy of Medical Sciences. ESCC cell line Ec9706 was obtained from Fuxiang biotechnology of Shanghai (Shanghai, China). Cells were maintained in Dulbecco's modi ed Eagle's medium (DMEM) or RPMI 1640 (Gibco) supplemented with 10% fetal bovine serum (Gibco), 100 units of penicillin/ml (Solarbio) and 100 mg of streptomycin/ml (Solarbio) in humidi ed air at 37°C with 5% CO2. HPV16 E6 plasmid and negative control (HPV16 E6 empty vector plasmid) were designed and synthesized by Heyuan company of Shanghai. Cell transfections were performed with Lipofectamine 2000 reagent (QIAGEN company) by following manufacturers' instructions.
Hu J., Yu J., Miao T., Zheng S., Cui X., Hu J., Yang L., & Li F. (2021). HPV 16 E6 Promotes Growth and Metastasis of Esophageal Squamous Cell Carcinoma cells in vitro.
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