Clampex pclamp 10.0 software

Extracellular stimulation was delivered using a stimulus isolation unit (A.M.P.I.) using glass monopolar electrodes (0.5–1 MΩ) filled with ACSF. fEPSPs were recorded in current-clamp mode with a MultiClamp 700B amplifier (Molecular Devices) driven by the Clampex (pClamp 10.0) software (Molecular Devices) using ACSF-filled patch pipettes (0.5–1 MΩ). The stimulating electrode was placed in the CA3 area and the recording electrode in the dendritic CA1 area. Experiments were performed at 29 ± 1 °C with flow rates of 2 ml per minute. 5 consecutive stimulations were delivered at 5, 10, 20, and 50 Hz. Stimulation intensity was set to acquire an initial fEPSP of approximately 0.3 mV. In total, 5–10 sweeps were conducted for each stimulus frequency and recordings were averaged over trials. Data were sampled at 10 kHz, amplified (gain 5), filtered at 3 kHz then digitized and analyzed using Clampfit. Data were included if the stimulation intensity to fEPSP ratio was within the linear range and if there was an increase in the amplitude of the second response. Data were averaged per slice, and then across the number of specified slices. Up to 3 slices were usable per each mouse.

Extracellular stimulation was delivered using a stimulus isolation unit (A.M.P.I) using glass monopolar electrodes (0.5–1 MΩ) filled with aCSF. fEPSPs were recorded in current-clamp mode with a MultiClamp 700B amplifier (Molecular Devices) driven by the Clampex (pClamp 10.0) software (Molecular Devices) using aCSF-filled patch pipettes (0.5–1 MΩ). The stimulating electrode was placed in the CA3 area and the recording electrode in the dendritic CA1 area. Experiments were performed at 29 ± 1 °C with flow rates of 2 ml per minute. Five consecutive stimulations were delivered at 5, 10, and 20 Hz. Stimulation intensity was set to acquire an initial fEPSP of approximately 0.3 mV. 5–10 sweeps were conducted for each stimulus frequency and recordings were averaged over trials. Data were sampled at 10 kHz, amplified (gain 5), filtered at 3 kHz then digitized and analyzed using Clampfit. Data were included if the stimulation intensity to fEPSP ratio was within the linear range and if there was an increase in the amplitude of the second response. Data were averaged per slice, and then across the number of specified slices. Up to 3 slices were usable per mouse.