Infinity 1200 series system

The content of tricin in the ETZL was determined by HPLC using an Agilent Infinity 1200 series system with a diode array detector (Agilent Technologies, Palo Alto, CA, USA). The separation of tricin was conducted at 30 °C using a SUPELCO Discovery^® C18 column (4.6 × 250 mm, 5 μm, Merck KGaA, Darmstadt, Germany). The mobile phase composition was as follows: A, 0.15% phosphoric acid in water; B, methanol. The gradient elution conditions were as follows: 0–3 min, 20% B; 3–8 min, 20–50% B; 8–20 min, 50–55% B; 20–30 min, 55–85% B; 30–45 min, 85–20% B. The flow rate was 1.0 mL/min and the injection volume was 10 μL. The chromatograms were obtained at 350 nm and UV-Vis spectra were acquired in 190–400 nm (Figure 1). The content of tricin was 1.09 ± 0.01 mg/g in the ETZL.

HPLC analysis was applied to determine the ginsenoside content of the P. ginseng sprouts using an Agilent Infinity 1200 series system with a diode array detector (Agilent Technologies, Palo Alto, CA, USA). The separation was performed at 25 °C using a SUPELCO Discovery^® C18 column (4.6 × 250 mm, 5 μm, Merck KGaA, Darmstadt, Germany). The mobile phase was composed of distilled water and acetonitrile. The gradient dilution conditions were as follows: 0–10 min, 20% acetonitrile; 10–20 min, 20–30% acetonitrile; 20–22 min, 30–70% acetonitrile; 22–29 min, 70–100% acetonitrile; 29–34 min, 100–100% acetonitrile; 34–35 min, 100–20% acetonitrile; and 35–38 min, 20–20% acetonitrile. The flow rate was 1.6 mL/min and the injection volume was 5 μL. Chromatograms were obtained at a wavelength of 204 nm.