Cd8 fitc

Manufactured by Thermo Fisher Scientific

Sourced in United States

CD8-FITC is a fluorescently-labeled monoclonal antibody that binds to the CD8 protein, which is expressed on the surface of certain T cells. It can be used in flow cytometry applications to identify and quantify CD8-positive cells in a sample.

Automatically generated - may contain errors

Lab products found in correlation

Spelling variants of this product

Anti-mouse CD8-FITC (8 citations) Anti-mouse CD3e-FITC + anti-mouse CD8-PE (5 citations) FITC)-labeled anti-mouse CD8 (4 citations) FITC-conjugated rat anti-mouse CD8 antibody (3 citations) FITC-conjugated anti-CD8 mAb (2 citations) Anti-CD8-FITC antibody (2 citations) CD8-FITC (53-6.7), (2 citations) FITC)-conjugated anti-human CD8 mAb (2 citations) Anti-human CD8-FITC (2 citations) FITC-labelled anti-CD8 (1 citations)

45 protocols using cd8 fitc

PD-1/PD-L1 blockade modulates CD8+ T-cell responses

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human peripheral blood mononuclear cells from healthy donators were stimulated with 5 μg/ml phytohaemagglutinin (Sigma) for 2 days and then rested for 1 day. Cells were pretreated with DMSO or MB or 25 μg/ml pembrolizumab or 20 μg/ml nivolumab for 1 h and then seeded in a 96‐well plate precoated with 10 μg/ml of aCD3/aCD28, with media supplemented with 10 μg/ml of hPD‐L1 protein. Cells were fixed with 4% paraformaldehyde (PFA) and permeabilized with saponin (sigma, 47036). Cells were stained with CD8‐FITC (1:100, eBioscience, 11‐0081‐82), IL‐2‐PE (1:100, BioLegend, 500306), IFNγ‐PE (1:100, BioLegend, 502508), perforin‐PE (1:100, BioLegend, 353303), or GZMB‐Alexa Fluro (1:100, BioLegend, 515405), and cytokine production was measured by flow cytometric analysis by gating on CD8‐positive population.

Fan Z., Tian Y., Chen Z., Liu L., Zhou Q., He J., Coleman J., Dong C., Li N., Huang J., Xu C., Zhang Z., Gao S., Zhou P., Ding K, & Chen L. (2020). Blocking interaction between SHP2 and PD‐1 denotes a novel opportunity for developing PD‐1 inhibitors. EMBO Molecular Medicine, 12(6), e11571.

+ Open protocol

+ Expand

FACS Analysis of Immune Cell Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following antibodies were used for FACS analysis: CD274-PE, CD₄-FITC, and CD₈-FITC (eBioscience). The carboxyfluorescein succinimidyl ester (CFSE) for the proliferation assays was purchased from eBioscience. Cell samples were harvested, washed and stained with 5 μL of antibody for 30 min to avoid light exposure. Cells were washed, resuspended in 500 μL of PBS and transferred to FACS tubes for analysis. Cells were analyzed using a Canto II (BD Biosciences) FACS machine.

Yuan W., Deng D., Li H., Hu X., Shang X., Hou X., Jiang H, & He H. (2021). IFNγ/PD-L1 Signaling Improves the Responsiveness of Anti-PD-1 Therapy in Colorectal Cancer: An in vitro Study. OncoTargets and therapy, 14, 3051-3062.

+ Open protocol

+ Expand

Multiparameter Phenotyping of Immune Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Single-cell suspensions from the SVF of fat tissue, spleen, and blood samples were incubated in FcBlock (BD Biosciences) for 15 min at 4°C. The cells were then stained with fluorophore-conjugated antibodies for 20 min at 4°C in the dark. The antibodies were purchased from BD Biosciences (CD8-FITC, CD11c-FITC, NK1.1-FITC, CD4-PE/Cy7, CD45.2-APC), eBioscience (San Diego, CA) (NKG2D-PE, F4/80-PE/Cy7, CD11b-APC/eFluor780), or BioLegend (San Diego, CA) (CD3ε-Pacific Blue). Dead cells were excluded with 7-AAD staining (BD Biosciences). Flow cytometry data was acquired with FACSCantoII or LSRII flow cytometer (BD Biosciences) and analyzed with FlowJo (Treestar, Ashland, OR).

Chung J.J., Markiewicz M.A., Polić B, & Shaw A.S. (2014). Role of NKG2D in Obesity-Induced Adipose Tissue Inflammation and Insulin Resistance. PLoS ONE, 9(10), e110108.

+ Open protocol

+ Expand

CSF Immune Cell Profiling by Flow Cytometry

Check if the same lab product or an alternative is used in the 5 most similar protocols

Five milliliters of CSF were obtained by lumbar spinal tap and centrifuged at 550g at 4°C for 5 minutes. The supernatant was removed, and the pellet was resuspended and incubated at 4°C for 20 minutes with fluorochrome-labeled antibodies against CD4, CD8, CD20, CD25, C-C chemokine receptor type 6 (CCR6), HLA-DR, CD14, and CD45 (BD Pharmingen Mouse Anti-Human Antibodies: CD4 PerCP, CD8 FITC, CD 20 Alexa Fluor 700, CD25 PE-Cy7, CD196 (CCR6) PE, Anti-HLA-DR V450, and CD14 APC; eBioscience mouse Anti-Human Antibody: CD45 EF 605). Cells were washed and resuspended in 200 μL of fluorescence-activated cell sorting (FACS) buffer before FACS analysis on a FACSCanto-II. Analysis of cell subset counts was performed with FlowJo. All CSF and serum tests were performed with researchers blinded to the clinical diagnosis and MRI results. CSF samples were measured by flow cytometry in a time frame of 90 minutes after lumbar spinal tab. CSF supernatants were stored in polypropylene tubes at −80°C until batched analysis of NfL and CHI3L1 concentrations.

Engel S., Friedrich M., Muthuraman M., Steffen F., Poplawski A., Groppa S., Bittner S., Zipp F, & Luessi F. (2019). Intrathecal B-cell accumulation and axonal damage distinguish MRI-based benign from aggressive onset in MS. Neurology® Neuroimmunology & Neuroinflammation, 6(5), e595.

+ Open protocol

+ Expand

Comprehensive Hematopoietic Cell Analysis Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Flow assisted cell sorting (FACS) was performed as described elsewhere (16 (link)) with the following conjugated antibodies; Mac1 PE, Gr1 FITC, B220 APC, B220 FITC, CD19 PE, surface IgM PE, CD43 FITC, CD3 PE, CD4 APC, CD8 FITC, CD71 PE, Ter119 FITC, Sca-1 PE, cKit FITC, cKit APC-Cy7, IL7Ra PECy7, CD34 FITC, CD16/32 PE (eBiosciences or BD Biosciences). StemSep Mouse Hematopoietic Progenitor Kit Lineage Positive antibody cocktail (Stem Cell Technologies, Vancouver, Canada) was used to detect lineage positive bone marrow cells, stained as previously described (16 (link)). Apoptosis assays were conducted using AnnexinV-FITC Apoptosis Detection Kit I following the manufacturer’s recommended protocol. IHC and immunoblotting were performed as previously described (49 (link)). Formalin-fixed paraffin embedded sections were stained with hematoxylin and eosin (H&E), myeloperoxidase (MPO) (A0398, DAKO), CD3 (MCA1477, AbD Serotec), B220 (553086, BD), Ter119 (116201, BioLegend). Stained slides were scanned and imaged as described elsewhere (49 (link)). For immunoblots, primary antibodies used were anti-V5 (ab9116, Abcam or R960-25, Life Technologies), and beta-actin (Cell Signaling Technology). Protein was visualized using Pierce ECL Western Blotting Substrate (Thermo Scientific, Illinois, USA) and Amersham Hyperfilm ECL (GE Healthcare Ltd, Buckinghamshire, UK).

Gough S.M., Lee F., Yang F., Walker R.L., Zhu Y.J., Pineda M., Onozawa M., Chung Y.J., Bilke S., Wagner E.K., Denu J.M., Ning Y., Xu B., Wang G.G., Meltzer P.S, & Aplan P.D. (2014). NUP98-PHF23 is a chromatin modifying oncoprotein that causes a wide array of leukemias sensitive to inhibition of PHD domain histone reader function. Cancer discovery, 4(5), 564-577.

+ Open protocol

+ Expand

Flow Cytometry Analysis of Immune Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mononuclear cells from the spleens and brains were surface-labeled with anti-mouse CD4 FITC (eBioscience, Cat #: 11-0042), CD25 PE (eBioscience, Cat #: 12-0251), NK1.1 PE (eBioscience, Cat #: 12-5941-82), CD8 FITC (eBioscience, Cat #: 11-0081-81), and B220 APC (eBioscience, Cat #: 17-0452-81). For Tregs labeling, the cells were further fixed and permeabilized using a Foxp3/transcription Factor Staining Buffer kit (eBioscience, Cat #: 00-5523) and then stained with anti-mouse Foxp3 APC (eBioscience, Cat #: 17-5773). Cells were washed and suspended in flow cytometry staining buffer (eBioscience, Cat #: 00-4222-57). FACS analysis was performed using Accuri C6 software (BD Biosciences, San Jose, CA, USA).

Gao W., Li F., Zhou Z., Xu X., Wu Y., Zhou S., Yin D., Sun D., Xiong J., Jiang R, & Zhang J. (2017). IL-2/Anti-IL-2 Complex Attenuates Inflammation and BBB Disruption in Mice Subjected to Traumatic Brain Injury. Frontiers in Neurology, 8, 281.

+ Open protocol

+ Expand

HIV Infection Analysis by Flow Cytometry

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mixed cell suspensions were stained for surface markers with combinations of the following antibodies: CD8-FITC, CD4-FITC, CD4-PE, CD3-APC, CD3-APC-Cy7 (eBioscience, San Diego, CA), CCR5-PE-Cy7 (BD Biosciences, San Jose, CA), CCR5-PE (R&D Systems, Minneapolis, MN), CCR6-PerCp-Cy5.5, CD90 Alexa Fluor-647 (Biolegend, San Diego, CA). For the HIV-infection experiments, following infection for 6–7 days, intracellular levels of p24 were analyzed as described previously^{8 (link)}. Briefly, after surface staining, cells were washed, fixed and permeabilized (20 min) following instructions provided in the Cytofix/Cytoperm Plus kit (BD Biosciences) and stained for intracellular p24 with KC57-FITC antibody (Beckman Coulter; Danvers, MA) for 30 min. Analysis was performed on BD FACSCalibur or BD FACSCanto flow cytometers (BD Biosciences) using FACSdiva software, and data analyzed with FlowJo software (Tree Star, Inc. Ashland, OR). Expression of surface markers was measured by the percentage of positive cells and the mean fluorescence intensity (MFI).

Rodriguez-Garcia M., Barr F.D., Crist S.G., Fahey J.V, & Wira C.R. (2014). Phenotype and Susceptibility to HIV-infection of CD4+ Th17 Cells in the Human Female Reproductive Tract. Mucosal immunology, 7(6), 1375-1385.

+ Open protocol

+ Expand

Isolation and Characterization of Tumor-Infiltrating Leukocytes

Check if the same lab product or an alternative is used in the 5 most similar protocols

Tumor tissue was washed several times in PBS, finely chopped with a razor blade and digested in HBSS containing 1mg/ml Collagenase type IV (Sigma-Aldrich, C5138),100ug/ml DNase I (Sigma-Aldrich, D5319), and 5% fetal calf serum for 10min at 37 ℃ with gentle rotation in shaker (150 rpm). Leukocytes were isolated from the supernatant with Percoll (Solarbio, P8370) gradient separation method in which the cells were responded in 40% Percoll and underlayered with 80% Percoll followed by centrifugation at 2500 rpm for 20min. For surface marker staining, cells were washed with PBS containing 0.5% BSA and stained with CD45-BV605 (103,140, Biolegend), F4/80-APC-cy7 (123,117, Biolegend), CD11b-APC (17-0112-82, eBioscience), CD11c-PE (12-0114-81, eBioscience), CD206-BV421 (141,717, Biolegend), Ly6G-PE-cy7 (127,617, Biolegend), CD3e-PerCP (100,325, Biolegend), CD4-AF700 (100,430, Biolegend) and CD8-FITC (11-0081-82, eBioscience) for 30 min in the dark. Serum levels of Data acquisition were performed on an LSR Fortessa instrument (BD Biosciences) and analyzed by using FlowJo software (Treestar) and SPSS26.0. IL-18 and IFN-γ were detected by enzyme-linked immunosorbent assay (ELISA, MultiScience).

Fan X., Yan Z., Lin Y., Wang Q., Jiang L., Yao X., Dong L., Chen L., Zhao T., Zhao J., Hu H, & Wang H. (2024). Mechanism exploration of Zoledronic acid combined with PD-1 in the treatment of hepatocellular carcinoma. Cancer Immunology, Immunotherapy : CII, 73(4), 62.

+ Open protocol

+ Expand

Multiparametric flow cytometry for immune cell profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols

Flow cytometry was used to determine protein levels on living cells. The following extra-cellular antibodies were used: CD3-APC (clone: 17A2), CD8-FITC (clone: 53-6.7), Rat IgG1, κ-PE isotype control (clone: eBRG1), from eBioscience; CD8a-APC (clone: 53-6.7), PD-1 (CD279)-PE_Cy7 (clone: 29F.1a12), PD-L1 (CD274)-APC (clone: 10F.9G2), CD4-Alexafluor647 (clone: GK1.5), Rat IgG2a, κ-FITC (clone: RTK2758), Rat IgG2b-Alexafluor 647(clone: RTK4530), Rat IgG2b, κ-APC (clone: RTK4530), κ-FITC (clone:RTK2758), from Biolegend. For intracellular protein staining we used anti-IFN-γ-Alexafluor 647 (clone: XMG1.2), anti-IFN-γ-PE (clone: XMG1.2) from eBioscience. Cell viability was monitored with either propidium iodide, from Sigma-Aldrich, Fixable Viability Dye -eFluor780, from eBioscience, or Aquablue Live/Dead Stain from ThermoFisher Scientific; depending on the experiment requirements. Data were acquired on a Fortessa (BD Biosciences) or a CyAn ADP (Beckman Coulter) and analyzed using FlowJo v10.

Mastroianni J., Stickel N., Andrlova H., Hanke K., Melchinger W., Duquesne S., Schmidt D., Falk M., Andrieux G., Pfeifer D., Dierbach H., Schmitt-Graeff A., Meiss F., Boerries M, & Zeiser R. (2018). miR-146a controls immune response in the melanoma microenvironment. Cancer research, 79(1), 183-195.

+ Open protocol

+ Expand

Multiparameter Immune Cell Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols

CD3-percp-cy5.5 (Catalogue #45-0036-42), CD8-APC (Catalogue #17-0086-42), CD8-FITC (Catalogue #11-0086-42), CD14-APC (Catalogue #17-0149-42), CD56-FITC (Catalogue #4278380), CD16-PerCP-eFluor™710 (Catalogue #46-0168-42), CD11b-PerCP-eFluor™710 (Catalogue #46-0110-80), IFN-γ-PE (Catalogue #12-7319-42), TLR2-FITC (Catalogue #11-9922-41) and Mouse IgG1-PE (Catalogue #12-4714-81) were all bought from eBioscience. HLA-DR-PE (Catalogue #555812), IL-10-APC (Catalogue #554707), CXCR3-APC (Catalogue #550967) and IFN-γ-FITC (Catalogue #561053), Rat IgG2a-APC (Catalogue #554690) and mouse IgG-APC (Catalogue #5065947) were purchased from BD Biosciences. CD16-FITC (Catalogue #302006), HLA-DR-APC (Catalogue #307609), CD3-FITC (Catalogue #317306) and TLR4-APC (Catalogue #312815) were purchased from Biolegend. EP2-PE (Catalogue #10477) and Rabbit IgG-PE (Catalogue D5-1610) were purchased from Cayman Chemical.

Wang Y., Chen C., Qi J., Wu F., Guan J., Chen Z, & Zhu H. (2019). Altered PGE2-EP2 is associated with an excessive immune response in HBV-related acute-on-chronic liver failure. Journal of Translational Medicine, 17, 93.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!