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USP22 is a deubiquitinating enzyme that plays a role in the regulation of gene expression. It is responsible for the removal of ubiquitin modifications from target proteins, which can impact their stability and function.

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Lab products found in correlation

Polyvinylidene fluoride membrane, by Merck Group (1 mentions) Hrp labelled secondary antibody, by Santa Cruz Biotechnology (1 mentions) Bm chemiluminescence western blotting kit, by Roche (1 mentions) Cd133, by Abcam (1 mentions) Bca assay kit, by Thermo Fisher Scientific (1 mentions) Gapdh, by Santa Cruz Biotechnology (1 mentions) Rad51, by Cell Signaling Technology (1 mentions) Yh2ax, by Cell Signaling Technology (1 mentions) 6xhis, by Santa Cruz Biotechnology (1 mentions) Flag tag (flag), by Merck Group (1 mentions) β actin, by Cell Signaling Technology (1 mentions)

2 protocols using usp22

1

Protein Expression Analysis by Western Blot

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Extracts of cells or tissues were prepared. The protein concentration was determined by BCA assay kit (Thermo Scientific). Equal amounts of proteins (30 μg) were subjected to SDS-PAGE and transferred to polyvinylidene fluoride membranes (Millipore). The membranes were treated with 1% blocking solution in TBS for 1 h, and immunoblots were probed with the indicated antibodies: USP22 (Santa Cruz), BMI1 (Abcam), CD133 (Abcam), SOX2 (Abcam), P21 (Proteintech), ubH2A (CST), EZH2 (CST), H3K27Me3 (CST), tubulin (Santa Cruz), HA (Abmart) GAPDH (Santa Cruz), and actin (Santa Cruz) at 4°C overnight. Then the membranes were washed and incubated with HRP-labelled secondary antibodies (1:5,000; Santa Cruz). The fluorescence signals were detected by a BM Chemiluminescence Western Blotting kit (Roche). Densitometry quantification was calculated and analysed using ImageJ software.
Ma Y., Fu H.L., Wang Z., Huang H., Ni J., Song J., Xia Y., Jin W.L, & Cui D.X. (2017). USP22 maintains gastric cancer stem cell stemness and promotes gastric cancer progression by stabilizing BMI1 protein. Oncotarget, 8(20), 33329-33342.
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2

PALB2-BRCA2-Rad51 Complex Regulation

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PALB2 (Abcam, ab220861), BRCA2 (Abcam, ab27976), Rad51 (Cell Signaling Technology, 8875S), USP22 (Santa Cruz, sc-390585), FLAG (Sigma, F3165-1MG), β-Actin (Cell Signaling Technology, 4970S), mCherry (Origene, TA180028), yH2A.X (Cell Signaling Technology, 2577S), MBP (New England Biolabs, E8032S), 6X-His (Santa Cruz, sc-8036), ENY2 (Abcam, ab183622), ATXN7L3 (Bethyl, A302-800A), ATXN7 (Bethyl, A302-638A).
Nardi I.K., Stark J.M., Larsen A., Salgia R, & Raz D.J. (2019). USP22 interacts with PALB2 and promotes chemotherapy resistance via homologous recombination of DNA double strand breaks. Molecular cancer research : MCR, 18(3), 424-435.
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