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Cd19 bv510 clone sj25c1

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The CD19-BV510 clone SJ25C1 is a fluorochrome-conjugated monoclonal antibody that binds to the CD19 antigen. CD19 is a B-lymphocyte-specific surface antigen that serves as a pan-B-cell marker. The BV510 fluorochrome can be detected using appropriate instrumentation.

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Lab products found in correlation

Cd3 percp clone sk7, by BioLegend (2 mentions) Cd4 apc clone okt4, by BioLegend (2 mentions) Cd8 bv570 clone rpa t8, by BioLegend (2 mentions) Cd14 fitc clone 63d3, by BioLegend (2 mentions) Ghost dye a710 viability stain, by Cytek Biosciences (2 mentions) Facsaria fusion instrument, by BD (2 mentions)

Spelling variants of this product

CD19 (SJ25C1, (11 citations) CD19-BV510 (8 citations)

2 protocols using cd19 bv510 clone sj25c1

1

Detailed PBMC Immunophenotyping Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
1M PBMCs from each donor were stained using standard procedure (30 min, 4 C) with the following surface antibody panel (CD3-PerCP clone SK7 (BioLegend), CD4-APC clone OKT4 (BioLegend), CD8-BV570 clone RPA-T8 (BioLegend), CD14-FITC clone 63D3 (BioLegend), CD19-BV510 clone SJ25C1 (BD), and Ghost dye A710 viability stain (Tonbo)) (Life Sciences Reporting Summary). Samples were then analyzed and sorted using a BD FACSAria Fusion instrument at the UCSF flow cytometry core. To calculate cell type proportions, the number of events in each of CD3+ CD4+ CD8− (CD4+ T cells), CD3+ CD4− CD8+ (CD8+ T cells), CD3− CD19+ (B cells), and CD3− CD14+ (monocytes) were divided by the sum of events in these gates (Supplementary Fig. 21).
Kang H.M., Subramaniam M., Targ S., Nguyen M., Maliskova L., McCarthy E., Wan E., Wong S., Byrnes L., Lanata C., Gate R., Mostafavi S., Marson A., Zaitlen N., Criswell L.A, & Ye C.J. (2017). Multiplexed droplet single-cell RNA-sequencing using natural genetic variation. Nature biotechnology, 36(1), 89-94.
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2

Detailed PBMC Immunophenotyping Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
1M PBMCs from each donor were stained using standard procedure (30 min, 4 C) with the following surface antibody panel (CD3-PerCP clone SK7 (BioLegend), CD4-APC clone OKT4 (BioLegend), CD8-BV570 clone RPA-T8 (BioLegend), CD14-FITC clone 63D3 (BioLegend), CD19-BV510 clone SJ25C1 (BD), and Ghost dye A710 viability stain (Tonbo)) (Life Sciences Reporting Summary). Samples were then analyzed and sorted using a BD FACSAria Fusion instrument at the UCSF flow cytometry core. To calculate cell type proportions, the number of events in each of CD3+ CD4+ CD8− (CD4+ T cells), CD3+ CD4− CD8+ (CD8+ T cells), CD3− CD19+ (B cells), and CD3− CD14+ (monocytes) were divided by the sum of events in these gates (Supplementary Fig. 21).
Kang H.M., Subramaniam M., Targ S., Nguyen M., Maliskova L., McCarthy E., Wan E., Wong S., Byrnes L., Lanata C., Gate R., Mostafavi S., Marson A., Zaitlen N., Criswell L.A, & Ye C.J. (2017). Multiplexed droplet single-cell RNA-sequencing using natural genetic variation. Nature biotechnology, 36(1), 89-94.
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