We pretreated all samples with formic acid. The following primary antibodies were used: a mouse monoclonal anti-p-tau antibody (1:100 dilution, Innogenetics, Ghent, Belgium). The signals from monoclonal and polyclonal antibodies were detected by using the automatic system on a VENTANA NX20 with the I-View DAB Universal Kit (Roche, Basel, Switzerland) according to the manufacturer’s instructions. Sections were counter-stained with hematoxylin.
Ventana nx20
Manufactured by Roche
Sourced in Switzerland, Japan
The VENTANA NX20 is a laboratory instrument designed for the automated staining of tissue samples. Its core function is to provide a standardized and reproducible process for the application of reagents to prepared tissue sections on glass slides. The VENTANA NX20 is capable of performing a variety of immunohistochemical and in situ hybridization assays.
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Lab products found in correlation
2 protocols using ventana nx20
1
Immunohistochemical Detection of p-Tau
2
Automated HER2 and HER3 Immunohistochemistry
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Immunohistochemistry was performed using the automated staining system, Ventana NX20 (Roche Diagnostics K.K., Tokyo, Japan) at SRL Co. Ltd. Rabbit polyclonal anti‐HER2 (Dako Denmark A/S, Glostrup, Denmark) and rabbit polyclonal anti‐HER3 (Santa Cruz Biotechnology, Inc., Dallas, TX, USA) antibodies were used. These antibodies were detected using an iVIEW DAB Detection Kit (Roche Diagnostics K.K.). Immunohistochemistry samples without anti‐HER2 or anti‐HER3 antibodies were used as negative controls. Protein expression was evaluated using light microscopy with low (×100), intermediate (×200), and high (×400) magnifications.
Positive expression was defined as strong expression of the examined protein in > 30% of lung cancer cells compared to adjacent lung epithelial cells. Expression was defined as 1+, 2+ and 3+ in cases of weak and membrane staining, complete but moderate membrane staining, and strong and complete staining, respectively.
Positive expression was defined as strong expression of the examined protein in > 30% of lung cancer cells compared to adjacent lung epithelial cells. Expression was defined as 1+, 2+ and 3+ in cases of weak and membrane staining, complete but moderate membrane staining, and strong and complete staining, respectively.
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