Ac histone h3

A549 cells were lysed in PRO-PREPTM protein extraction solution (iNtRON Biotechnology, Seongnam, Korea). Lysates were separated by 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis and transferred onto polyvinyl difluoride membranes (Millipore Inc., Billerica, MA). Membranes were blocked with a 5% skim milk solution and incubated with the following antibodies: E-cadherin, vimentin, α-SMA, fibronectin, snail, slug (Santa Cruz, CA), HDAC2, HDAC4, ac-histone H3, histone H3, ac-histone H4, histone H4 (Upstate, Millipore Inc.), and β-actin (Santa Cruz, CA). The blots were visualized with HRP-conjugated secondary antibodies and an ECL system (Pierce, Rockford, IL).

HDAC inhibitors (Table 1) were solubilized in dimethyl sulfoxide (DMSO) (Sigma Chemical, St Louis, MO, USA). The primary antibodies used include the following: α-tubulin (sc-32293, Santa Cruz Biotechnology, Santa Cruz, CA, USA), histone H3 (sc-10809, Santa Cruz), GAPDH (AP0066, Bioworld Technology, Bloomington, MN, USA), SMC3 (A300-060A-M, Bethyl Laboratories, Montgomery, TX, USA), ac-SMC3 (MABE1073, Millipore, Burlington, MA, USA), ac-histone H3 (06-599, Millipore), HDAC8 (ab187139, Abcam, Cambridge, UK), HIF-1α (610959, BD PharMingen, San Diego, CA, USA), GLUT1 (#12939, Cell Signaling Technology, Danvers, MA, USA), and HK2 (#2867, Cell Signaling Technology).