Anti ki67 antibody sp6

Manufactured by Abcam

Sourced in China, United States, United Kingdom

Anti-Ki67 antibody (SP6) is a primary antibody that binds to the Ki-67 protein, a well-established marker of cellular proliferation. This antibody is widely used in research and clinical applications to detect and quantify the presence of proliferating cells.

Automatically generated - may contain errors

Lab products found in correlation

In situ apoptosis detection kit, by Abcam (1 mentions) Anti e cadherin, by R&D Systems (1 mentions) Anti vimentin, by Merck Group (1 mentions) Vectastain elite abc hrp kit, by Vector Laboratories (1 mentions) Alexa fluor 488 conjugated anti mouse igg antibody, by Thermo Fisher Scientific (1 mentions) Anti nik antibody, by Abcam (1 mentions) Eclipse ti inverted microscope, by Nikon (1 mentions) Hoechst 33342, by Merck Group (1 mentions) Deadend colorimetric tunel system, by Promega (1 mentions) Fv1000 confocal microscope, by Olympus (1 mentions) Ab16667, by Abcam (1 mentions)

Spelling variants of this product

Anti-Ki67 (637 citations) Anti-Ki67 antibody (259 citations) Ki67 antibody (174 citations) Ki67 (SP6, (13 citations) Ki67 [SP6] antibody (3 citations) Rabbit anti-Ki67 antibody [SP6] (3 citations)

7 protocols using anti ki67 antibody sp6

Immunohistochemical Analysis of Xenograft Tumors

Check if the same lab product or an alternative is used in the 5 most similar protocols

Formalin-fixed and paraffin-embedded 6 μm-thick tissue sections from xenograft tumors were processed for immunohistochemistry analysis as per the standard protocol. Sections were stained with anti-Ki67 antibody (SP6; Abcam) using the peroxidase technique. The proliferation index (%) was determined by calculating the number of Ki67-positive cells relative to the total number of cells, which consisted of at least 1000 cells per field. Serial sections were stained with anti-E-cadherin (1:100 dilution; R&D Systems) and anti-vimentin (1:100 dilution; Sigma) and the immune complexes were detected using the Vectastain Elite ABC-HRP kit (Vector Laboratories, Burlingame, CA). The sections were counterstained with hematoxylin.
TUNEL staining was performed to measure apoptosis in tumor tissue sections using In Situ Apoptosis Detection Kit (Abcam) according to the manufacturer’s instructions. The apoptosis index (%) was determined by calculating the number of TUNEL-positive cells relative to the total number of cells, which consisted of at least 1000 cells per field.

Lee Y., Ko D., Yoon J., Lee Y, & Kim S. (2021). TMEM52B suppression promotes cancer cell survival and invasion through modulating E-cadherin stability and EGFR activity. Journal of Experimental & Clinical Cancer Research : CR, 40, 58.

+ Open protocol

+ Expand

Quantifying Tumor Cell Proliferation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Formalin-fixed and paraffin-embedded 6-μm-thick tissue sections from xenograft tumors were processed for immunohistochemistry analysis as per the standard protocol. Sections were stained with anti-Ki67 antibody (SP6; Abcam) using the peroxidase technique. The proliferative index (%) was determined by calculating the number of Ki67⁺ cells relative to the total number of cells, which consisted of at least 1,000 cells per field. Five randomly selected fields from tumor sections per mouse were analyzed using ImageJ software.

Ko D., Kim E., Shin E.A., Nam S.H., Yoon J., Lee J.S., Lee Y., Park S., Ha K., Choi S.Y., Lee J.W, & Kim S. (2022). Therapeutic effects of TM4SF5-targeting chimeric and humanized monoclonal antibodies in hepatocellular and colon cancer models. Molecular Therapy Oncolytics, 24, 452-466.

+ Open protocol

+ Expand

Hepatocellular Carcinoma Induction Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

AI was purchased from Chiatai Qingchunbao Pharmaceutical Co., Ltd. (Hangzhou, China).
N-diethylnitrosamine (DEN) was purchased from TCI Chemicals Co., Ltd.
(Shanghai, China). Piperonyl butoxide (PBO) was purchased from Aladdin^®(Shanghai, China). Anti-glutathione S-transferase placental form (GST-P)
pAb was purchased from Medical and Biological Laboratories Co., Ltd. (1:1000; Nagoya,
Japan). Anti-Ki67 antibody [SP6] was purchased from Abcam (1:500; Shanghai, China). The
real-time PCR primers listed in Table
1Table 1. Sequence of Primers Used for Real-time Reverse Transcription-polymerase Chain<br/>Reaction (RT-PCR) Analysis were designed and synthesized by Thermo Fisher Scientific (Chengdu,
China).

Tang Q., Zhang M., Hong Z., Chen Y., Wang P., Wang J., Wang Z., Fang R, & Jin M. (2019). Effects of astragalus injection on different stages of early hepatocarcinogenesis in a two-stage hepatocarcinogenesis model using rats. Journal of Toxicologic Pathology, 32(3), 155-164.

+ Open protocol

+ Expand

Multimodal Histopathological Analysis of Liver and Colon

Check if the same lab product or an alternative is used in the 5 most similar protocols

After fixation with 10% neutral buffered formalin, liver and colon samples were paraffin-embedded and 2-μm-sectioned. H&E staining of liver fixation was used to assess the progression of liver disease, while H&E staining of colon specimens was used to evaluate gut structural integrity and changes. In addition, hepatic fibrogenesis was evaluated by Sirius red staining (Ma et al., 2023) (link). Hepatic cell proliferation and Kupffer cells were evaluated by immunohistochemical analysis with anti-Ki-67 antibody [SP6] (Abcam, USA) (Li et al., 2023a) (link) and recombinant anti-F4/80 antibody [EPR26545-166] (Abcam), respectively. Furthermore, Alcian blue-periodic acid-Schiff (AB-PAS) staining was performed to trace goblet cells according to previously described protocols (Gao et al., 2022) (link). Sections from the proximal colon were used to explore tight-binding proteins by immunofluorescence. After being dewaxed and rehydrated, sections were incubated with claudin-3 antibodies purchased from Proteintech (USA) at 4°C overnight. In addition, secondary antibodies were combined with washed samples at room temperature for 1 h. After rinsing, the sections were sealed and then captured by a Nikon Eclipse Ci epifluorescence microscope (Nikon, Japan).

Jiang S., Xu L., Chen Y., Shu Z., Lv L., Zhao Y., Bi K., Yang S., Wang Q, & Li L. (2024). Longitudinal gut fungal alterations and potential fungal biomarkers for the progression of primary liver disease. Science China. Life sciences, 67(6).

+ Open protocol

+ Expand

Immunofluorescence and Immunohistochemical Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

For immunofluorescence staining, fixed and permeabilized cells were stained with an anti-DSG1 antibody (129204, R&D Systems), followed by an Alexa Fluor 488-conjugated anti-mouse IgG antibody (Invitrogen). Nuclei were visualized by Hoechst 33342 (Sigma-Aldrich) staining. An FV1000 confocal microscope (Olympus) and accompanying FV10-ASW software were used to acquire confocal images. For immunohistochemical staining, fixed and paraffin-embedded tumor sections were stained with hematoxylin and eosin (HE), anti-Ki-67 antibody (SP6, Abcam), and anti-NIK antibody (Abcam), using standard procedures. TUNEL staining was performed with the DeadEnd Colorimetric TUNEL System (Promega). Images were captured with an Eclipse Ti inverted microscope (Nikon) and accompanying NIS-Elements software. Quantification was performed using ImageJ software. The number of Ki-67⁺ or TUNEL⁺ cells in each field was counted. NIK expression in tumor cells was scored (intensity: 0 = negative, 1 = weak, 2 = moderate, 3 = strong), and the H score was calculated using the following equation: H score = 1 × (% of cells with intensity 1) + 2 × (% of cells with intensity 2) + 3 × (% of cells with intensity 3).

Yang T., Sim K.Y., Ko G.H., Ahn J.S., Kim H.J, & Park S.G. (2022). FAM167A is a key molecule to induce BCR-ABL-independent TKI resistance in CML via noncanonical NF-κB signaling activation. Journal of Experimental & Clinical Cancer Research : CR, 41, 82.

+ Open protocol

+ Expand

Tumor Measurement and Survival Endpoint

Check if the same lab product or an alternative is used in the 5 most similar protocols

Tumors were measured 6 days post inoculation and the volumes were calculated from the formula, V = (ab²)/2, where a is the length of the tumor (mm), and b is the width of the tumor (mm). The survival end point of each mouse was determined by either spontaneous death or the presence of moribund signs. To ensure the welfare of the animals, the tumor size must not exceed 20 mm in any direction in each HCC-bearing mouse. If multiple tumors are presented, the combination of the two largest diameters may not exceed 20 mm for mice. When the tumor size exceeds 20 mm in any direction, mice were considered as dead and were sacrificed. Immunohistochemical detection of Ki67 (anti-Ki67 SP6 antibody, Abcam, #ab16667) in tumor tissues were detected as described in the previous methods at fourteen days after immunization. In all experiments, treated groups were randomized to prevent cage effects.

Liu Q., Yang Y., Tan X., Tao Z., Adah D., Yu S., Lu J., Zhao S., Qin L., Qin L, & Chen X. (2017). Plasmodium parasite as an effective hepatocellular carcinoma antigen glypican-3 delivery vector. Oncotarget, 8(15), 24785-24796.

+ Open protocol

+ Expand

Evaluating Liposomal Hyperthermin Formulations in Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mice were randomly assigned into four groups (n=5). Saline, HYP (6.0 mg/kg), SPC/HYP-Lip (6.0 mg/kg, calculated by HYP content), or DLD/HYP-Lip (6.0 mg/kg, calculated by HYP content) was administrated via tail vein injection.31 Different formulations were injected at 12, 14, 16, 18, and 20 days after CBRH-7919 cell injection. Mice were weighed and tumors were measured every 2 days. Mice were sacrificed on day 25 after tumor inoculation and tumors were removed and then embedded in paraffin and cut with a microtome (5-mm thick) before staining with hematoxylin and eosin (HE). For quantification of proliferative cells, Ki67 was assessed with Anti-Ki67 [SP6] antibody (Abcam, England). The Ki67 index was calculated as the ratio of proliferative cells to total cells in each field, using five random fields. For quantification of apoptotic cells, tdt-mediated dutp nick-end labeling (TUNEL) assays were used with the in situ cell death detection kit-POD (Roche Group, Switzerland). The apoptotic index was calculated as the ratio of apoptotic cells to total cells in each field, using five random fields.

Feng Y., Qin G., Chang S., Jing Z., Zhang Y, & Wang Y. (2021). Antitumor Effect of Hyperoside Loaded in Charge Reversed and Mitochondria-Targeted Liposomes. International Journal of Nanomedicine, 16, 3073-3089.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!