Biotinylated-BSA (1 μg/μl, Thermo) is flowed for 25 min through the device, allowing its binding to the epoxy surface. On top of the biotinylated-BSA, 0.5 μg/μl of NutraAvidin (Pierce, Rockford, IL) is added (flow for 20 min). The ‘button’ valve is then closed, and biotinylated-PEG (1 μg/μl, (PG2-AMBN-5k, Nanocs Inc.) is flowed over for 20 min, passivating the flow layer, except for the buttons area. Following passivation, the ‘button’ valve is released and a flow of 0.2 μg/μl biotinylated anti-GFP antibodies (Abcam; #ab6658, Cambridge, United Kingdom) or 0.01 µg/µl biotinylated anti-Flag antibodies (Cell Signaling; #2908 S Danvers, MA, USA) were applied. The antibodies bound to the exposed NutraAvidin, specifically to the area under the ‘button’, creating an array of anti-GFP - or anti-Flag tag. PBS buffer was used for washing in between steps. In the case of p27 immobilization, surface chemistry was performed with 0.2 μg/ml donkey anti-mouse whole biotinylated anti-IgG antibodies (#715-065-150, Jackson Immuno research laboratories, Maryland, USA) followed by 20 min flow of 6.5 μg/ml anti p27 antibodies (Santa Cruz biotechnology, Heidelberg Germany; #1641 mouse).
Biotinylated anti flag antibodies
Manufactured by Cell Signaling Technology
Sourced in United Kingdom
Biotinylated anti-Flag antibodies are affinity-purified antibodies that have been conjugated with biotin. They are designed for the detection and enrichment of proteins tagged with the Flag epitope in various applications, such as immunoprecipitation, Western blotting, and affinity purification.
Automatically generated - may contain errors
2 protocols using biotinylated anti flag antibodies
1
Surface Bioconjugation Biomolecule Immobilization
2
Microfluidic Antibody Immobilization
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Biotinylated-BSA (1 μg/μl, Thermo) is flowed for 25 min through the device, allowing its binding to the epoxy surface. On top of the biotinylated-BSA, 0.5 μg/μl of Neutravidin (Pierce, Rockford, IL) is added (flow for 20 min). The 'button' valve is then closed, and biotinylated-PEG (1 μg/μl, (PG2-AMBN-5k, Nanocs Inc.) is flowed over for 20 min, passivating the flow layer, except for the buttons area. Following passivation, the 'button' valve is released and a flow of 0.2 μg/μl biotinylated anti-GFP antibodies (Abcam; #ab6658, Cambridge, United Kingdom) or 0.01 µg/µl biotinylated anti-Flag antibodies (Cell Signaling; #2908S Danvers, MA, USA) were applied. The antibodies bound to the exposed Neutravidin, specifically to the area under the 'button', creating an array of anti-GFP -or anti-Flag tag. PBS buffer was used for washing in between steps. In the case of p27 immobilization, surface chemistry was performed with 0.2 μg/ml donkey anti-mouse whole IgG antibodies (#715-065-150, Jackson Immuno research laboratories, Maryland, USA) followed by 20 min flow of 6.5 μg/ml anti p27 antibodies (Santa Cruz biotechnology, Heidelberg Germany; #1641 mouse).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!