Anti total erk1

Manufactured by Santa Cruz Biotechnology

Sourced in United States

Anti-total-ERK1 is a primary antibody that recognizes the extracellular signal-regulated kinase 1 (ERK1) protein. It detects the total amount of ERK1 present in a sample, regardless of its activation state.

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Lab products found in correlation

Wortmannin, by Fujifilm (1 mentions) α sma, by Agilent Technologies (1 mentions) Pd98059, by Cayman Chemical (1 mentions) Anti phospho erk, by Cell Signaling Technology (1 mentions) Anti phospho akt, by Cell Signaling Technology (1 mentions) Anti collagen type 1, by Rockland Immunochemicals (1 mentions) Anti total akt, by Cell Signaling Technology (1 mentions) Anti di phosphorylated erk 1 2, by Merck Group (1 mentions) C digit chemiluminescence scanner, by LI COR (1 mentions)

Close spelling variants of this product

Anti-total ERK1/2 (3 citations) Anti-(total)-ERK1/2 polyclonal antibody (2 citations)

2 protocols using anti total erk1

Endostatin Signaling Pathways in Wound Healing

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Reagent sources were as follows: Recombinant mouse endostatin (Sigma-Aldrich, St. Louis, MO, USA), PD98059 (Cayman, Ann Arbor, MI, USA), wortmannin (Wako, Osaka, Japan).
Antibodies sources were as follows: anti-phospho-Akt (Ser473), anti-total Akt, anti-phospho-ERK (Cell Signaling Technology, Beverly, MA, USA), anti-total-ERK1, anti-endostatin (Santa Cruz Biotech, Santa Cruz, CA, USA), anti-type I collagen (Rockland immunochemicals, Gilbertsville, PA, USA), anti-MMP-2 (Kyowa pharma chemical, Toyama, Japan), anti-total-glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (Wako), α-SMA (Dako, Glostrup, Denmark), anti-rabbit immunoglobulin G (IgG) horseradish peroxidase linked whole antibody, anti-mouse IgG horseradish peroxidase linked whole antibody (Amersham Bioscences, Buckinghamshire, UK).

Sugiyama A., Hirano Y., Okada M, & Yamawaki H. (2018). Endostatin Stimulates Proliferation and Migration of Myofibroblasts Isolated from Myocardial Infarction Model Rats. International Journal of Molecular Sciences, 19(3), 741.

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Western Blot Analysis of Phosphorylated ERK

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The samples were lysed in cold lysis buffer and the total homogenate (50 µg) was separated using 12% polyacrylamide gel. The proteins on nitrocellulose membrane were blocked with 5% non-fat dried milk and 0.1% Tween20 at RT and incubated overnight with primary antibodies 1/700 anti-diphosphorylated ERK1/2 (Sigma-Aldrich) and 1/1000 anti-total ERK1 (Santa Cruz Biotechnology, Inc). The blots were incubated with peroxidase-conjugated anti-rabbit (1/5000) or anti-mouse (1/2500 Jackson Immunoresearch Labs Inc) secondary antibodies and then revealed with ECL detection reagents (Inmun-Star HRP-Substrate Kits, Bio-Rad). Finally, the emitted light was captured by the C-DiGit Chemiluminescence Scanner (LI-COR Biosciences), and signals were quantified with ImageJ software.

Sosa L.D.V., Petiti J.P., Picech F., Chumpen S., Nicola J.P., Perez P., De Paul A., Valdez-Taubas J., Gutierrez S, & Torres A.I. (2019). The ERα membrane pool modulates the proliferation of pituitary tumours. The Journal of endocrinology, 240(2).

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