All reference standards for target compounds were of high purity grade (>99%). Methylisothiazolinone (MI), benzisothiazolinone (BIT), dimethyl phthalate (DMP), diethyl phthalate (DEP), and their internal standards (dimethyl phthalate-3,4,5,6-d4 and diethyl phthalate-3,4,5,6-d4) were obtained from Sigma-Aldrich, methylchloroisothiazolinone (CMI), and its internal standard benzoisothiazol-3-one-13C6 was supplied by Toronto Research Chemicals. Other solvents, including methyl tert-butyl ether (MTBE), were purchased from Duksan Pure Chemicals, South Korea. Dichloromethane (DCM), methanol, and ethyl acetate were purchased from Fisher Scientific Korea Ltd. HPLC grade water was supplied by Avantor Performance Materials Korea. Individual stock solutions of all target compounds and internal standards were prepared in ethanol and stored at −20 °C before use.
Benzisothiazolinone
Manufactured by Merck Group
Sourced in United States
Benzisothiazolinone is a chemical compound used in the manufacturing of laboratory equipment. It functions as a preservative, helping to prevent the growth of microorganisms in various laboratory solutions and samples.
Automatically generated - may contain errors
Lab products found in correlation
Diethyl phthalate, by Merck Group (1 mentions)
Dimethyl phthalate, by Merck Group (1 mentions)
Methylisothiazolinone, by Merck Group (1 mentions)
Diethyl phthalate 3 4 5 6 d4, by Merck Group (1 mentions)
Milli q system, by Merck Group (1 mentions)
Formic acid, by Thermo Fisher Scientific (1 mentions)
Methanol, by Thermo Fisher Scientific (1 mentions)
Acetonitrile, by Thermo Fisher Scientific (1 mentions)
2 protocols using benzisothiazolinone
1
Quantification of Biocides and Phthalates
2
Chromatographic Analysis of Preservatives
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The solvents used in the chromatographic method, methanol and acetonitrile (both HPLC grade), were purchased from Fisher Scientific® (Leicestershire, UK). Formic acid was obtained from Acros Organics® (Geel, Belgium) and water for the HPLC was dispensed by a Milli-Q system from Millipore (Bedford, MA, USA). The reference materials of MCI/MI (14.2%) and MI (98%) were obtained from Santa Cruz Biotechnology® (Dallas, Texas, USA), while benzisothiazolinone (BIT, 99.2%) and octylisothiazolinone (OIT, 99.9%) were purchased from Sigma Aldrich® (St. Louis, MO, USA). Using methanol as a solvent, a reference solution containing 10 µg/ml MI was prepared for injection along with reference solutions of MCI, BIT and OIT, all at a concentration level of 1.5 µg/ml.
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