Rna assay kit

Manufactured by Thermo Fisher Scientific

Sourced in United States

The RNA Assay Kit is a laboratory tool designed to quantify RNA content in a sample. It provides a standardized method to measure RNA concentration and purity. The kit includes reagents and protocols to enable accurate RNA analysis in a research or diagnostic setting.

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Lab products found in correlation

Qubit 2.0 fluorometer, by Thermo Fisher Scientific (6 mentions) Rna nano 6000 assay kit, by Agilent Technologies (3 mentions) Bioanalyzer 2100 system, by Agilent Technologies (3 mentions) Trizol reagent, by Thermo Fisher Scientific (3 mentions) 2100 bioanalyzer, by Agilent Technologies (2 mentions) Nanophotometer spectrophotometer, by Implen (2 mentions) Nanodrop, by Thermo Fisher Scientific (2 mentions) Nanodrop 1000 spectrophotometer, by Thermo Fisher Scientific (2 mentions) Dna blood kit, by Qiagen (1 mentions) Qubit dsdna hs assay kit, by Thermo Fisher Scientific (1 mentions) Rneasy column, by Qiagen (1 mentions) Dneasy tissue kit, by Qiagen (1 mentions) Qiaamp dna micro kit, by Qiagen (1 mentions) Rneasy extraction kit, by Qiagen (1 mentions) Rrna removal kit, by Illumina (1 mentions) Hiseq 2000 platform, by Illumina (1 mentions) Trizol reagent, by Takara Bio (1 mentions) Small rna kit, by Agilent Technologies (1 mentions) Hiseq platform, by Illumina (1 mentions) Truseq pe cluster kit v3 cbot hs, by Illumina (1 mentions)

Close spelling variants of this product

TaqMan® Small RNA Assays Kit (8 citations) RNA ISH tissue assay kits (3 citations) Qubit dsDNA and RNA high-sensitivity assay kits (2 citations) Qubit RNA BR and HS Assay Kits (2 citations) Qubit™ RNA High Sensitivity (HS) and Broad Range (BR) Assay Kits (2 citations)

15 protocols using rna assay kit

Tissue Sample Preparation for Mutational Analysis

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We selected representative native tumor samples and paraffin embedded tissue for mutational analysis and confirmed vital tumor content microscopically in each case. DNA was extracted using the DNeasy tissue kit and the QIAamp DNA micro kit purchased from Qiagen (Hilden, Germany). Pooled DNA obtained from peripheral blood leukocytes of healthy persons were extracted with the Blood DNA kit (Qiagen) according to the manufacturer’s instructions and served as wild type controls. RNA from snap frozen tissue was isolated with the RNeasy extraction kit (Qiagen) followed by subsequent digestion with RNase-free DNase I and purification via RNeasy columns (Qiagen).
DNA and RNA concentration was determined using the Qubit® dsDNA HS Assay Kit or RNA Assay Kit (Life Technologies, Eugene, USA).

Hölsken A., Sill M., Merkle J., Schweizer L., Buchfelder M., Flitsch J., Fahlbusch R., Metzler M., Kool M., Pfister S.M., von Deimling A., Capper D., Jones D.T, & Buslei R. (2016). Adamantinomatous and papillary craniopharyngiomas are characterized by distinct epigenomic as well as mutational and transcriptomic profiles. Acta Neuropathologica Communications, 4, 20.

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RNA-Seq Analysis of HFHSD Pig Model

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RNA isolation, library construction, and sequencing were performed by Novogene Corporation. RNA extraction was performed according to the manufacturer’s instructions and was quantified using a Bioanalyzer 2100 (Agilent Technologies, CA, USA). The quality and quantity of RNA were assessed using an RNA Assay Kit and a fluorometer (Life Technologies, CA, USA). RNA was frozen and stored at −80 °C immediately after production.
A total quantity of 3 μg RNA per sample was used as the input material for the RNA sample preparations from three HFHSD pigs (number as 120, 138, 146) and three control pigs (number as 157, 159, 161). First, ribosomal RNA was removed using an rRNA Removal Kit (Epicentre, WI, USA), and the rRNA-depleted sample was cleaned. Subsequently, sequencing libraries were generated. To select cDNA fragments between 150–200 bp in length, the library fragments were purified with an appropriate system (Beckman Coulter, Beverly, USA). Subsequently, USER enzyme was incubated with the size-selected, adaptor-ligated cDNA samples. PCR was performed using Phusion High-Fidelity DNA polymerase, Universal PCR primers, and an Index Primer. Finally, the products were purified and library quality was assessed. After cluster generation, the libraries were sequenced on an Illumina Hiseq 2000 platform, and 100-bp paired-end reads were generated.

Xia J., Xin L., Zhu W., Li L., Li C., Wang Y., Mu Y., Yang S, & Li K. (2016). Characterization of long non-coding RNA transcriptome in high-energy diet induced nonalcoholic steatohepatitis minipigs. Scientific Reports, 6, 30709.

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Total RNA Extraction and Characterization

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The total RNA was extracted by grinding the cells in TRIzol reagent (TaKaRa, Japan) in liquid nitrogen, isolated with chloroform, and precipitated with isopropanol, and then the sediment was washed with 75% alcohol and dissolved in RNA-free distilled water. RNA degradation was detected on 1% agarose gels, and the purity was checked using a Nanodrop 2000C spectrophotometer. Furthermore, the concentration of RNA was measured using an RNA Assay Kit in Qubit (Life Technologies, CA, USA). The RNA integrity was assessed using an RNA Nano 6000 Assay Kit with the Bioanalyzer 2100 system (Agilent Technologies, CA, USA).

Guo Y., Lu B., Tang H., Bi D., Zhang Z., Lin L, & Pang H. (2019). Tolerance against butanol stress by disrupting succinylglutamate desuccinylase in Escherichia coli. RSC Advances, 9(21), 11683-11695.

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RNA Quantification and Characterization

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Subsequently, RNA yields were measured using the Qubit 2.0 Fluorometer (Life Technologies, Germany) in combination with the RNA Assay Kit (Life Technologies, Germany) according to the manufacturer's protocol. The maximum volume of sample input was used (20 µl), standards were freshly prepared and the Qubit was equilibrated after the manufacturer's instructions. A Bioanalyzer 2100 (Agilent Technologies, Germany) run using the Small RNA Kit (Agilent Technologies, Germany) was performed afterwards for the analysis and quantification of RNA eluates resolving small RNAs in the range from 6 to 150 nts length. The extracted RNA was stored at −80°C until further analysis.

Spornraft M., Kirchner B., Haase B., Benes V., Pfaffl M.W, & Riedmaier I. (2014). Optimization of Extraction of Circulating RNAs from Plasma – Enabling Small RNA Sequencing. PLoS ONE, 9(9), e107259.

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Transcriptome Analysis of Cotton Diploids

Cited 2 times

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G. arboreum and G. herbaceum were used for the transcriptome sequencing. The RNA-Seq libraries were prepared by a previously described method (Wang et al., 2018 (link)), and a 1% agarose gel was used to check for contamination and degradation of RNA. First, the purity of RNA was analyzed using a Nano Photometer® spectrophotometer (IMPLEN, CA, United States). Next, estimating RNA concentration was performed using a Qubit® RNA Assay Kit and a Qubit® 2.0 Fluorometer (Life Technologies, CA, United States). Finally, RNA integrity was checked using an Agilent Nano 6000 assay kit (Santa Clara, California, United States). Reads counting features (genes, in this case) were performed using HTSeq v0.6.125. Gene lengths and read counts mapped to genes were used to calculate FPKM values (Mortazavi et al., 2008 (link)). The original data was uploaded to NCBI (PRJNA833579).
The differentially expressed genes (DEGs) between diploid and tetraploid were identified with the DESeq R package (Andino et al., 2016 (link)), and Benjamini–Hochberg-adjusted p-values < 0.05 were considered statistically significant (Benjamini and Hochberg, 1995 (link); Anders and Huber, 2010 (link)).

Wang H., Umer M.J., Liu F., Cai X., Zheng J., Xu Y., Hou Y, & Zhou Z. (2022). Genome-Wide Identification and Characterization of CPR5 Genes in Gossypium Reveals Their Potential Role in Trichome Development. Frontiers in Genetics, 13, 921096.

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Transcriptome Analysis of Sea Bass Intestines

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Intestines were obtained from the sea bass in the two Groups 24 h after infection. Total RNA of the two groups was extracted using the TRIzol method. RNA degradation and contamination were monitored on 1% agarose gels. RNA concentration was measured using a Qubit^® RNA Assay Kit in a Qubit^® 2.0 Fluorometer (Life Technologies, CA, United States). A total amount of 1 μg RNA per sample was used as input material for the RNA sample preparations. Sequencing libraries were generated using the NEBNext^® UltraTM RNA Library Prep Kit for Illumina^® (NEB, United States) following the manufacturer’s recommendations, and index codes were added to attribute sequences to each sample. The clustering of the index-coded samples was performed on a cBot Cluster Generation System using TruSeq PE Cluster Kit v3-cBot-HS (Illumina) according to the manufacturer’s instructions. After cluster generation, the library preparations were sequenced on an Illumina HiSeq platform, and 125 bp/150 bp paired-end reads were generated. High-throughput transcriptome sequencing was completed by Wuhan Metware Biotechnology Co., Ltd.

Pan C., Zhu Y., Cao K., Li J., Wang S., Zhu J., Zeng X., Zhang H, & Qin Z. (2023). Transcriptome, intestinal microbiome and histomorphology profiling of differences in the response of Chinese sea bass (Lateolabrax maculatus) to Aeromonas hydrophila infection. Frontiers in Microbiology, 14, 1103412.

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RNA-seq of Silk Moth Development

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RNA-seq was achieved for four samples representing four stages of S. montelus (egg, larva, pupa, and adult; Figure 1) to assist in the gene predictions in the genome annotation step. Total RNA from each sample was collected using an RNeasy Plus Mini Kit (Qiagen, Germany). The RNA purity was checked using a NanoDrop One/OneC spectrophotometer (Thermo Fisher Scientific). RNA degradation and contamination were monitored using 1% agarose gels. The RNA concentration was measured using a Qubit RNA Assay Kit of a Qubit 3.0 Fluorometer (Life Technologies, CA, USA). The RNA integrity was assessed using an RNA Nano 6000 Assay Kit of a Bioanalyzer 2100 system (Agilent Technologies, CA, USA). The RNA quality for the RNA samples with an integrity value more than 8.0 was used for constructing the cDNA library. The paired-end library was prepared using an Illumina TruSeq Sample Preparation Kit and sequenced on a NovaSeq6000 platform (Illumina). The raw data were filtered by removing reads containing adapters, Ns, and low-quality reads using fastp v0.20.0 (Chen et al., 2018 (link)).

Li J., Wang H., Zhu J., Yang Q., Luan Y., Shi L., Molina-Mora J.A, & Zheng Y. (2023). De novo assembly of a chromosome-level reference genome of the ornamental butterfly Sericinus montelus based on nanopore sequencing and Hi-C analysis. Frontiers in Genetics, 14, 1107353.

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Total RNA Isolation from Tissues

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Total RNA was isolated from the breast muscle and liver tissues using the TRIzol reagent (Invitrogen, Waltham, MA, USA) according to the manufacturer’s instructions. The degradation and contamination of the RNA were monitored on 1% agarose gels. The RNA integrity was assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, Santa Clara, CA, USA). The concentration of the total RNA was measured using a Qubit^® RNA Assay Kit in Qubit^® 2.0 Fluorometer (Life Technologies, Carlsbad, CA, USA) and the purity was checked using the NanoPhotometer^® spectrophotometer (IMPLEN, Westlake Village, CA, USA).

Yin Z., Zhou W., Mao H., Dong X., Huang X., Zhang H, & Liu H. (2022). Identification of Genes Related to Squab Muscle Growth and Lipid Metabolism from Transcriptome Profiles of Breast Muscle and Liver in Domestic Pigeon (Columba livia). Animals : an Open Access Journal from MDPI, 12(9), 1061.

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RNA Extraction and Purification for Cells

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Total RNAs were extracted from cells with liquid nitrogen by using RNAiso Plus reagent (TaKaRa Bio, Inc., Japan) and puri ed using a QIAGEN RNeasy Mini Kit (QIAGEN, Chatsworth, CA, USA) according to the manufacturer's instructions. RNA degradation and contamination were monitored on 1.5% agarose gels.
The purity and concentration of the RNAs were measured using NanoPhotometer® spectrophotometer (IMPLEN, Los Angeles, CA, USA) and a Qubit RNA Assay Kit in a Qubit® 2.0 Fluorometer (Life Technologies, Carlsbad, CA, USA), respectively. All RNA samples were stored at -80℃ until further use.

Kyei B., Li L., Yang L., Zhan S., Li J., Chen Y., Zhang Q., Zheng S., Xu X., Odame E., Cao J., Guo J., Zhong T., Wang L., & Zhang H. (2020). CDR1as related miRNA-mRNA networks in differentiating goat skeletal muscle satellite cells.

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Formulation of Lipid Nanoparticles for mRNA Delivery

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After diluting the purified mRNA in citrate buffer (pH 4.0) to the desired concentration, the lipids ionizable lipid (Dlin-MC3-DMA), distearoylphosphatidylcholine (DSPC), cholesterol, and PEG-lipid (PEG2000-DMG) were dissolved in ethanol at a molar ratio of 50:10:38.5:1.5, and the water and lipid phases were mixed at a volumetric ratio of 3:1 with a microfluidics mixer (NanoAssemblr, Canada) to formulate lipid nanoparticles (LNPs). The LNPs were dialyzed in phosphate-buffered saline (pH 7.4) to remove ethanol for 24 h and concentrated to a desired concentration using centrifugal filters (Merck, Ireland). Finally, the LNPs were passed through a 0.22-μm filter and stored at 4 °C until use. The particle size and zeta potential of the LNPs were measured by a Malvern instrument (Malvern, England), and their encapsulation efficiency was determined by an RNA Assay Kit (Thermo Fisher Scientific).

Sun W., He L., Zhang H., Tian X., Bai Z., Sun L., Yang L., Jia X., Bi Y., Luo T., Cheng G., Fan W., Liu W, & Li J. (2021). The self-assembled nanoparticle-based trimeric RBD mRNA vaccine elicits robust and durable protective immunity against SARS-CoV-2 in mice. Signal Transduction and Targeted Therapy, 6, 340.

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