Pu 2 087 plus pumps

Preparative HPLC was conducted with a Jasco LC-2000 Plus Series system equipped with two PU 2,087 Plus pumps (flow up to 50 ml min⁻¹) and a diode array detector (MD-2010 Plus 195–650 nm). The purification of the compounds was performed in reverse phase mode with a 218TP Vydac C18 column (22 × 250 mm, Grace Davison Discovery Science).

Preparative HPLC was performed on a Jasco HPLC system (Jasco GmbH, Germany) consisting of two Jasco PU-2087 Plus pumps connected to a Jasco UV-2075 Plus variable wavelength detector set at 260nm, an Advantec CHF122SC fraction collector (Tokyo Seisakusho Kaisha Ltd, Japan), and an Agilent LC/MSD Trap XCT mass spectrometer. The (HP)LC column was a Synergi 4u Fusion-RP 80, 25 cm×21.5mm (Phenomenex). The HPLC solvents were 0.1% formic acid in water (A) and 0.1% formic acid in MeOH (B). For separation of compounds dissolved in 100% MeOH, a gradient was used from 100% A for 2min, then to 100% B in 28min, 20min at these conditions, returning to 100% A at a flow rate of 9.5ml min^–1. The injection volume was 950 μl per HPLC run. Fractions (9.5ml) were collected at one fraction per minute. Data analysis was performed using the ChromPass Version 1.9.302.1124 software (Jasco GmbH, Germany).