Anti β catenin pab

The following primary monoclonal (mAb) and polyclonal (pAb) antibodies were used to detect cytoskeletal, junctional, and leukocyte proteins: anti-p120-catenin and E-cadherin, Ly-6 and CD4 mAbs (BD Biosciences, San Jose, CA); anti-occludin, JAM-A, ZO-1, Claudin-1 and 7 pAbs, and Claudin-4 mAb (Life Technologies, Waltham, MA); anti-total actin (clone C4) mAb (EMD Millipore, Billerica, MA); anti-GAPDH (14C10) mAb and active caspase 3 pAb (Cell Signaling, Beverly, MA); anti-β-catenin pAb (Sigma-Aldrich, Saint Lois, MO); anti-α-catenin mAb (Abcam, Cambridge, MA); anti-NM IIA, NM IIB and NM IIC pAbs (Covance, Princeton, NJ); F4/80 mAb (Bio-Rad Laboratories, Hercules, CA); goat anti-E-cadherin pAb (R&D Systems, Minneapolis, MN); anti- lysozyme pAb (Santa Cruz, Dallas, TX); FITC conjugated rat anti-mouse IgA (eBioscience, San Diego, CA). Alexa Fluor-488-conjugated donkey anti-rabbit and donkey anti-goat, Alexa Fluor-555-conjugated donkey anti-mouse and goat anti-rat secondary antibodies, and Alexa Fluor-488 and 555-labeled phalloidin were obtained from Life Technologies. Horseradish peroxidase-conjugated goat anti-rabbit and anti-mouse secondary antibodies were acquired from Bio-Rad Laboratories. All other chemicals were obtained from Sigma-Aldrich.