Qtrap5500 hybrid system

Manufactured by AB Sciex

Sourced in Japan

The QTRAP 5500 hybrid system is a high-performance mass spectrometer that combines the benefits of a triple quadrupole and an ion trap. It provides high sensitivity, high resolution, and accurate quantitation for a wide range of analytical applications.

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Lab products found in correlation

Uflc xr device, by Shimadzu (4 mentions) Turbo vtm ion source, by AB Sciex (4 mentions)

4 protocols using qtrap5500 hybrid system

Quantification of Bile Acid Profiles

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BAs were extracted from bile, portal and systemic plasma by precipitation with iced methanol.³² The BA species were quantified by high-performance liquid chromatography (UFLC-XR device, Shimadzu) coupled to tandem mass spectrometry (QTRAP5500 hybrid system, equipped with a Turbo VTM ion source, Sciex) using 5 deuterated BAs (d4-cholic acid [CA], d4-glycocholid acid, d4-taurocholic acid, d4-CDCA, d4-glycochenodeoxycholic acid) as internal standards. CA, CDCA, ursodeoxycholic acid, αMCA and βMCA are mouse primary BAs. DCA, LCA, ωMCA and hyodeoxycholic acid are mouse secondary BAs.

Gillard J., Clerbaux L.A., Nachit M., Sempoux C., Staels B., Bindels L.B., Tailleux A, & Leclercq I.A. (2021). Bile acids contribute to the development of non-alcoholic steatohepatitis in mice. JHEP Reports, 4(1), 100387.

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Quantification of Bile Acids by HPLC-MS/MS

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Bile acids were extracted from portal plasma by precipitation with ice-cold methanol. The bile acid species were quantified by high-performance liquid chromatography (UFLC-XR device, Shimadzu, Kyoto, Japan) coupled to tandem mass spectrometry (QTRAP5500 hybrid system, equipped with a Turbo VTM ion source, Sciex) using five deuterated bile acids (d4-CA, d4-GCA, d4-TCA, d4-CDCA, d4-GCDCA) as internal standards, as previously described [27 (link)].

Gillard J., Picalausa C., Ullmer C., Adorini L., Staels B., Tailleux A, & Leclercq I.A. (2022). Enterohepatic Takeda G-Protein Coupled Receptor 5 Agonism in Metabolic Dysfunction-Associated Fatty Liver Disease and Related Glucose Dysmetabolism. Nutrients, 14(13), 2707.

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Plasma Bile Acid Profiling by LC-MS/MS

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Plasma concentrations of 21 BA species (Table S1) and 7alpha-hydroxy-4-cholesten-3-one (C4, an intermediate product of the classical BA synthesis pathway and marker of hepatic BA synthesis) were quantified as previously described.¹⁷ (link) Briefly, after protein precipitation with iced methanol, BAs were quantified by HPLC (UFLC-XR device; Shimadzu, Kyoto, Japan) coupled to tandem mass spectrometry (MS/MS) (QTRAP 5500 hybrid system, equipped with a Turbo VTM ion source; Sciex, Foster City, CA, USA) using 5 deuterated BAs (D4-CA, D4-glycocholic acid [GCA], D4-taurocholic acid [TCA], D4-CDCA, D4-glycochenodeoxycholic acid [GCDCA]) as internal standards. After isolation using a SPE column, C4 was quantified by LC-MS/MS using a deuterated C4 as internal standard. Plasma BA and C4 concentrations were expressed in nmol/L. Ratio and total values were determined according to formulas presented in Table S1.

Grzych G., Chávez-Talavera O., Descat A., Thuillier D., Verrijken A., Kouach M., Legry V., Verkindt H., Raverdy V., Legendre B., Caiazzo R., Van Gaal L., Goossens J.F., Paumelle R., Francque S., Pattou F., Haas J.T., Tailleux A, & Staels B. (2020). NASH-related increases in plasma bile acid levels depend on insulin resistance. JHEP Reports, 3(2), 100222.

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Quantification of Bile Acid Profiles

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Plasma concentrations of the bile acids (BAs) were determined after protein precipitation with iced methanol as previously published [34 (link)]. BAs from the cecal content were quantified after extraction on samples lyophilized at −80 °C to avoid bacterial BA. In both biological samples, the 27 BA species were quantified by high-performance liquid chromatography (UFLC-XR device, Shimadzu, Kyoto, Japan) coupled to tandem mass spectrometry (MS/MS) (QTRAP 5500 hybrid system, equipped with a Turbo VTM ion source, Sciex, Foster City, CA, USA) using five deuterated BAs (D4-CA, D4-GCA, D4-TCA, D4-CDCA, D4-GCDCA) as internal standards. Plasma and cecal content BA concentrations were expressed in nmol/l and as a percentage of total BAs, respectively. Ratio and total values were determined according to formulas presented in Table S4.

Alard J., Cudennec B., Boutillier D., Peucelle V., Descat A., Decoin R., Kuylle S., Jablaoui A., Rhimi M., Wolowczuk I., Pot B., Tailleux A., Maguin E., Holowacz S, & Grangette C. (2021). Multiple Selection Criteria for Probiotic Strains with High Potential for Obesity Management. Nutrients, 13(3), 713.

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