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Mouse cytokine chemokine magnetic bead panel

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The Mouse Cytokine/Chemokine Magnetic Bead Panel is a multiplex assay designed to simultaneously measure the levels of multiple mouse cytokines and chemokines in a single sample. The panel utilizes magnetic beads coated with antibodies specific to the target analytes, allowing for the detection and quantification of these important signaling molecules.

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Lab products found in correlation

Magpix system, by DiaSorin (2 mentions) Ifn β, by PBL Assay Science (2 mentions) Macs octo dissociator, by Miltenyi Biotec (2 mentions) Np40 lysis buffer, by Thermo Fisher Scientific (2 mentions) Cxcl1, by RayBiotech (2 mentions) Interleukin 6 (il 6), by R&D Systems (2 mentions) Luminex 100 system, by Merck Group (2 mentions) Hmgb1, by Shino-Test (2 mentions) Milliplex map kit for mouse metabolic magnetic bead panel, by Merck Group (2 mentions) Serum insulin, by Crystal Chem (1 mentions) Xponent software version 3, by DiaSorin (1 mentions) Luminex 200 system, by DiaSorin (1 mentions) Creatinine, by Fujifilm (1 mentions) Magpix instrument, by DiaSorin (1 mentions) Prism 8, by GraphPad (1 mentions) Luminex 200, by R&D Systems (1 mentions) Bio plex 5.0 manager software, by Bio-Rad (1 mentions) Bio plex 200 system, by Bio-Rad (1 mentions) Luminex xmap technology, by DiaSorin (1 mentions) Bessman tissue pulverizer, by Thermo Fisher Scientific (1 mentions)

19 protocols using mouse cytokine chemokine magnetic bead panel

1

Multiplex Cytokine Profiling in Serum

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The concentrations of cytokines and chemokines, GM-CSF, IL-6, TNF-α, IL-2, IFN-γ, IL-1β, MIP-1β, IL-13, IL-4, IL-5, IL-17A, MCP-1, IL-10, IL-12 (p70), and IP-10 in serum were determined using Mouse Cytokine/Chemokine Magnetic Bead Panel (Millipore) via Luminex xMAP, according to manufacturer’s instructions.
Cheng M., Xu J., Ding K., Zhang J., Lu W., Liu J., Gao J., Alugupalli K.R, & Liu H. (2022). Attenuation of relapsing fever neuroborreliosis in mice by IL-17A blockade. Proceedings of the National Academy of Sciences of the United States of America, 119(42), e2205460119.
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2

Multiplex Cytokine and Chemokine Profiling

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Mouse Cytokine/Chemokine Magnetic Bead Panel was purchased from Milipore (Billerica, MA). The 96-well plate kit was customized to measure CC chemokine ligand (CCL) 2, also known as monocyte chemoattractant protein-1 (MCP-1), and CC chemokine ligand (CCL) 5, also known as regulated on activation, normally T-cell expressed and secreted (RANTES), in plasma and tumor and vascular endothelial growth factor-a (VEGF-a) and VEGF-c in tumor (14 (link), 30 (link)). Further information is described in Supplementary Data.
Song G., Darr D.B., Santos C.M., Ross M., Valdivia A., Jordan J.L., Midkiff B.R., Cohen S., Feinberg N.N., Miller C.R., Tarrant T.K., Rogers A.B., Dudley A.C., Perou C.M, & Zamboni W.C. (2014). Effects of Tumor Microenvironment Heterogeneity on Nanoparticle Disposition and Efficacy in Breast Cancer Tumor Models. Clinical cancer research : an official journal of the American Association for Cancer Research, 20(23), 6083-6095.
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3

Cytokine/Chemokine Analysis in Skin

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Four hours after pDNA electrotransfer, mice were humanely euthanized and skin samples snap-frozen on dry ice. The samples were homogenized in NP-40 Lysis buffer (Alfa Aesar, Ward Hill, MA, USA) with the addition of Halt Protease Inhibitor cocktail EDTA-free (Thermo Fisher Scientific, Rockford, IL, USA) using a gentle MACS OctoDissociator (Miltenyi Biotec, Auburn, CA, USA). The total protein concentration was normalized, and 25 μg of each sample was analyzed using a Mouse Cytokine/Chemokine Magnetic Bead Panel (Millipore, Burlington, MA, USA) using a MAGPIX System (Luminex, Austin, TX, USA) per manufacturer’s instructions. Confirming ELISAs to quantify IFN-β (PBL Assay Science, Piscataway, NJ, USA), IL-6 (R&D Systems, Minneapolis, MN, USA), and CXCL1 (RayBiotech, Peachtree Corners, GA, USA) were performed on the same homogenates.
Bosnjak M., Znidar K., Sales Conniff A., Jesenko T., Markelc B., Semenova N., Tur J., Kohena K., Kranjc Brezar S., Heller L, & Cemazar M. (2022). In vitro and in vivo correlation of skin and cellular responses to nucleic acid delivery. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 150, 113088.
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4

Measuring Oxidative Stress Biomarkers in Serum

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The levels of HMGB1 and RAGE were measured using commercially available enzyme-linked immunosorbent assay kits (HMGB1: Shino-test, Kanagawa, Japan; RAGE: R&D Systems, Minneapolis, MN, USA). Interleukin 6 (IL-6), TNF-α and chemokine (C-X-C motif) ligand 1 (CXCL-1) were measured with a Mouse Cytokine/Chemokine-Magnetic Bead Panel (Millipore, Billerica, MA, USA) in a Luminex 100 system (Millipore). The serum concentration of hydroperoxides (whole oxidant capacity of serum against N,N-diethylparaphenylene-diamine in acidic buffer) was measured as described previously [27 (link)]. The measurement unit was CARR U. It has been previously established that 1 CARR U corresponds to 0.08 mg hydrogen peroxide/dL [30 (link)].
Nosaka N., Yashiro M., Yamada M., Fujii Y., Tsukahara H., Liu K., Nishibori M., Matsukawa A, & Morishima T. (2015). Anti-high mobility group box-1 monoclonal antibody treatment provides protection against influenza A virus (H1N1)-induced pneumonia in mice. Critical Care, 19(1), 249.
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5

Analgecine's Immune Response Effects

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To determine the effects of Analgecine on the immune response, we detected transforming growth factor (TGF)-β expression using the TGF-β Magnetic Bead Kit (Millipore, Billerica, MA, USA) and IL-6, IL-10, IL-12, tumor necrosis factor (TNF)-α and interferon (IFN)-γ expression using the Mouse Cytokine/Chemokine Magnetic Bead Panel (Millipore, Billerica, MA, USA) with mouse plasma according to the manufacturer's instructions. The protocol for preparation of plasma samples were showed in Supplementary Materials.
Chen X., Zhuang X., Zhang Q., Luo Y., Yuan S, & Qiao T. (2017). Analgecine enhances the anti-tumor response of radiotherapy by increasing apoptosis and cell cycle arrest in non-small cell lung cancer. Oncotarget, 8(46), 80730-80740.
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6

Cytokine Analysis in Kidney Tissue

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Kidney tissues at 20 weeks were washed with phosphate-buffered saline and were homogenized on ice. Tissue homogenates were centrifuged to remove tissue residues at 12.000 g for 10 minutes at 4°C. Total protein concentration of the supernatant was measured using Pierce BCA protein assay kit. The cytokines were analyzed in the supernatant separated from tissue residues and plasma by using Luminex-bead array (Mouse Cytokine/Chemokine Magnetic Bead Panel, Millipore, Billerica, MA, USA) for detection of IFN-γ, TNF-α, IL-4, IL-6, and IL-17 according to the manufacturer's specifications. Concentrations of all cytokines measured were expressed as pg/mL.
Seo J.W., Kim Y.G., Lee S.H., Lee A., Kim D.J., Jeong K.H., Lee K.H., Hwang S.J., Woo J.S., Lim S.J., Kim W, & Moon J.Y. (2015). Mycophenolate Mofetil Ameliorates Diabetic Nephropathy in db/db Mice. BioMed Research International, 2015, 301627.
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7

Metabolic and Inflammatory Biomarkers in Mice

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At week 12 and 24, mice were fasted overnight and sacrificed for subsequent analysis. Blood was collected into microfuge tubes and allowed to clot for 30 min. Then samples were centrifuged at 3000 rpm for 20 min and serum was collected and stored at − 80 °C until analysis. Serum insulin (CrystalChem Inc.) was quantified by ELISA. The homeostasis model assessment of insulin resistance (HOMA-IR) index was calculated based on the following formula: fasting insulinemia (μUI/ml) × fasting glycaemia (mM)/22.5. The concentrations of inflammatory cytokines IL-1β, IL-6 and TNF-α were quantified using the Mouse Cytokine/Chemokine Magnetic Bead Panel (Millipore). The Milliplex MAP Kit for Mouse Metabolic Magnetic Bead Panel (Millipore) was used to quantify hormones including resistin, gastric inhibitory polypeptide (GIP) and leptin.
He C., Yang Z., Cheng D., Xie C., Zhu Y., Ge Z., Luo Z, & Lu N. (2016). Helicobacter pylori Infection Aggravates Diet-induced Insulin Resistance in Association With Gut Microbiota of Mice. EBioMedicine, 12, 247-254.
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8

Cytokine/Chemokine Analysis in Skin

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Four hours after pDNA electrotransfer, mice were humanely euthanized and skin samples snap-frozen on dry ice. The samples were homogenized in NP-40 Lysis buffer (Alfa Aesar, Ward Hill, MA, USA) with the addition of Halt Protease Inhibitor cocktail EDTA-free (Thermo Fisher Scientific, Rockford, IL, USA) using a gentle MACS OctoDissociator (Miltenyi Biotec, Auburn, CA, USA). The total protein concentration was normalized, and 25 μg of each sample was analyzed using a Mouse Cytokine/Chemokine Magnetic Bead Panel (Millipore, Burlington, MA, USA) using a MAGPIX System (Luminex, Austin, TX, USA) per manufacturer’s instructions. Confirming ELISAs to quantify IFN-β (PBL Assay Science, Piscataway, NJ, USA), IL-6 (R&D Systems, Minneapolis, MN, USA), and CXCL1 (RayBiotech, Peachtree Corners, GA, USA) were performed on the same homogenates.
Bosnjak M., Znidar K., Sales Conniff A., Jesenko T., Markelc B., Semenova N., Tur J., Kohena K., Kranjc Brezar S., Heller L, & Cemazar M. (2022). In vitro and in vivo correlation of skin and cellular responses to nucleic acid delivery. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 150, 113088.
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9

Multiplex Assay for Mouse Metabolic Hormones

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Milliplex MAP Kit for Mouse Metabolic Magnetic Bead Panel (Catalogue# MMHMAG-44K; Millipore Corp., MO, USA) was used to quantify eight gut hormones that are important regulators of food intake, energy expenditure and body mass: acylated (active) ghrelin, leptin, active amylin, insulin, active glucagon-like peptide-1 (GLP-1), total gastric inhibitory polypeptide (GIP), total peptide YY (PYY) and pancreatic polypeptide (PP). Mouse Cytokine/Chemokine Magnetic Bead Panel (Catalogue# MPXMCYTO-70K; Millipore Corp., MO, USA) was used to measure a panel of 32 different mouse cytokines/chemokines. Plasma was used and assays were carried out according to manufacturer's instructions. Measurement was performed on Luminex 200™ system with xPONENT™ software, version 3.1 (Luminex, Austin, TX, USA).
Khosravi Y., Seow S.W., Amoyo A.A., Chiow K.H., Tan T.L., Wong W.Y., Poh Q.H., Sentosa I.M., Bunte R.M., Pettersson S., Loke M.F, & Vadivelu J. (2015). Helicobacter pylori infection can affect energy modulating hormones and body weight in germ free mice. Scientific Reports, 5, 8731.
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10

Multiplexed Cytokine Profiling in Tumor-Bearing Mice

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Mouse serum samples and tumor-conditioned media, derived from in vitro cultured tumor cell lines, were analyzed by Mouse Cytokine/Chemokine Magnetic bead panel (Millipore, Billerica, USA) according to manufacturer's instructions. Serum samples from tumor-bearing mice were normalized to naïve wild-type mice.
Eggert T., Medina-Echeverz J., Kapanadze T., Kruhlak M.J., Korangy F, & Greten T.F. (2014). Tumor Induced Hepatic Myeloid Derived Suppressor Cells Can Cause Moderate Liver Damage. PLoS ONE, 9(11), e112717.
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