Osteogenic cells were fixed with an acetone/citrate buffer pH 4.2 (1.5:1) for 5 min at room temperature. Cells were stained with Napthol-AS-TR-phosphate solution (Sigma-Aldrich, Hamburg, Germany) for 1 h at room temperature. The staining solution consists of 1:1 v/v Napthol-AS-TR-phosphate solution (Napthol-AS-TR-phosphate diluted 1:5 in H2O) and Fast Red TR solution (Sigma-Aldrich ApS, Hamburg, Germany) (diluted 1:1.2 in 0.1 M Tris buffer, pH 9.0).
Napthol as tr phosphate solution
Manufactured by Merck Group
Sourced in Germany
Napthol-AS-TR-phosphate solution is a laboratory reagent commonly used as a substrate in various enzymatic assays. It serves as a chromogenic indicator, undergoing a color change upon enzymatic cleavage. The solution provides a standardized substrate for detecting and measuring enzymatic activity in research and diagnostic applications.
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Lab products found in correlation
3 protocols using napthol as tr phosphate solution
1
Osteogenic Cell Staining Protocol
2
Osteogenic Lineage Induction and Staining
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Cells were induced to osteogenic lineage and fixed with acetone/citrate buffer pH 4.2 (1.5:1) for 5 min at room temperature. Cells were stained with Napthol-AS-TR-phosphate solution (Sigma-Aldrich ApS) for 1 h at room temperature. Napthol-AS-TR-phosphate solution consists of Napthol-AS-TR-phosphate diluted 1:5 in H2O and Fast Red TR (Sigma-Aldrich ApS) diluted 1:1.2 in 0.1 M Tris buffer, pH 9.0, after which both solutions were mixed 1:1 and applied to the cells.
3
Naphthol-AS-TR-phosphate Staining Protocol
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Cells were fixed with acetone/citrate buffer pH 4.2 (1.5:1) for 5 min at room temperature and stained with Napthol-AS-TR-phosphate solution (Sigma-Aldrich ApS) for 1 h at room temperature. Napthol-AS-TR-phosphate solution consists of Napthol-AS-TR-phosphate diluted 1:5 in H2O and Fast Red TR (Sigma-Aldrich ApS) diluted 1:1.2 in 0.1 M Tris buffer, pH 9.0, after which both solutions were mixed 1:1.
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