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5 protocols using 1 2 benzenedithiol

1

Synthesis and Characterization of Silver Nanoparticles

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All chemicals, including silver nitrate (AgNO3, 99%), 1,2-benzenedithiol (1,2-BDT), sodium borohydride (NaBH4, 99.99% metals basis), and tetraoctylammonium bromide (TOAB),
were purchased from Sigma-Aldrich and used without further purification.
Solvents, including methanol, dichloromethane (DCM), dimethylformamide
(DMF), and acetonitrile, were used from Sigma as received. Distilled
water (H2O) was obtained from Milli-Q (Millipore apparatus).
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2

Quantitative Analysis of Sulfatase Activity

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Sulfatase from Helix pomatia, sinigrin hydrate, l-ascorbic acid (AA), trichloroacetic acid (TCA), dl-dithiothreitol (DTT), N-ethylmaleimide (NEM), 2,2′-bipyridyl, iron (III) chloride (FeCl3), 3-O-CQA (3-O-caffeoylquinic acid), 5-O-CQA (5-O-caffeoylquinic acid), butyl-isothiocyanate, caffeic acid (CA), 1,2-benzenedithiol, ß-mercaptoethanol (HPLC grade), methylene chloride (HPLC grade), sephadex A-25, sephadex C-25, acetonitrile (HPLC grade), methanol (HPLC grade), sulforaphane, sodium acetate, potassium phosphate, and orthophosphoric acid (H3PO4) were obtained from Sigma Chemical Co. (St. Louis, MO, USA). Desulfoglucoraphanin was obtained from Santa Cruz Biotechnology (Dallas, TX, USA).
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3

Analytical Protocols for Botanical Extracts

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Imidazol formate, formic acid, acetonitrile, sulfatase, 2-propanol, 2,2-azinobis-(ethyl-2,3-dihydrobenzothiazoline-6-sulphonic acid) diammonium salt (ABTS), 1,2-benzenedithiol, 3,3′-diindolylmethane (DIM), and indole-3-carbinol (I3C) were obtained from Sigma-Aldrich (Darmstadt, Germany). Glucotropaeolin (GTL) was from AppliChem (Darmstadt, Germany), indole-3-acetonitrile (I3ACN) from Merck (Darmstadt, Germany), and HPLC grade methanol from Chempur (Piekary Śląskie, Poland). Water was purified with a QPLUS185 system from Millipore (Burlington, MA, USA).
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4

Purification and Storage of Organic Compounds

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1,2‐Benzenedithiol was purchased from Sigma‐Aldrich, purified by vacuum distillation, and stored in small aliquots at −20°C. Sulforaphane, a major dietary ITC, was purchased from LKT Laboratories. HPLC grade Methanol was purchased from Fisher Chemical.
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5

Antioxidant Capacity Determination Protocol

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Methanol and ferric chloride were purchased from Panreac (Barcelona, Spain). Gallic acid, ascorbic acid, hydrochloric acid, 2,4,6-tris(2-pyridyl)-s-triazine (TPTZ), 2,2diphenyl-1-picrylhydrazyl (DPPH), tris(2-carboxyethyl)phosphine hydrochloride (TCEP), potassium phosphate monobasic, potassium phosphate dibasic, calcium chloride, 1,2-benzenedithiol, α-amylase (EC 3.2.1.1), pepsin (EC 3.4.23.1) , pancreatin, fresh bile, sodium hydroxide, ammonium sulphate, and sodium carbonate were purchased from Sigma-Aldrich (Steinheim, Germany). Folin-Ciocalteu's reagent was purchased from VWR (Llinars del Vallès, Spain). All reagents used were of analytical grade.
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