Samples were analyzed using an HPLC system (Nexera XR, Shimadzu, Kyoto, Japan) coupled to a Triple TOF 6600 mass spectrometer (AB SCIEX, Foster City, CA, USA). Data acquisition and analysis were conducted using Peak View and Multi Quant software. Chromatographic separation was performed on an Agilent 5 TC-C18 (2) column (4.6 mm × 250 mm, 5 μm) maintained at 50 °C by gradient elution using 0.1% formic acid in water (solvent A) and 0.1% formic acid in ACN/MeOH (50/50, v/v) (solvent B) delivered at 1 mL/min. The gradient elution program was set as follows: 0−2.0 min 10% → 70% B; 2.0−15.0 min 70% → 95% B; 15.0−16.0 min 95% B; 16.0−16.1 min 95% → 10% B; and 16.1−20.0 min 10% B. The MS parameters are given in Supporting Information Table S1 .
5 tc c18 2 column
Manufactured by Agilent Technologies
Sourced in United States
The 5 TC-C18 (2) column is a high-performance liquid chromatography (HPLC) column designed for the separation and analysis of a wide range of chemical compounds. It features a C18 stationary phase, which is commonly used for the separation of non-polar to moderately polar analytes. The column dimensions are 5 μm particle size and 4.6 mm x 150 mm.
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2 protocols using 5 tc c18 2 column
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HPLC-MS/MS Analysis of Compounds
2
HPLC Determination of Compounds
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Chromatographic determinations were performed using a HPLC system equipped with a LC-20AT quaternary gradient pump, DGU-20A5 degasser, SIL-20A autosampler, CTD-10ASvp thermostatted column compartment, CBM-20A communication bus module, and SPD-M20A detector (Shimadzu, Kyoto, Japan). Chromatographic separation was performed using an Agilent 5 TC-C18(2) column (250 mm × 4.6 mm, 5.0 μm, Santa Clara, CA, USA). The mobile phase components were 0.1% formic acid (A) and acetonitrile (B) at a flow rate of 1.0 mL/min. The following gradient elution program was used: 0~13 min, 89~85% A; 13~25 min, 85~75% A; 25~27 min, 75~73% A; 27~45 min, 73% A. The column temperature was set at 32 °C and the injection volume was 10 μL. Additionally, the chromatographic data at a wavelength of 325 nm were applied.
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