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2 protocols using nitrofurantoin f

1

Antimicrobial Susceptibility Testing via Kirby-Bauer Disk Diffusion

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Antimicrobial susceptibility testing was done using the Kirby-Bauer disk diffusion method on Mueller Hinton agar (Oxoid Ltd.) prepared with 4mm thickness.13
Panels of eleven antimicrobial disks including ampicillin (10µg), amoxicillin-Clavulanic acid (10µg), cefoxitin (30µg), ciprofloxacin (5µg), gentamicin (10µg), norfloxacin (10µg), cefepime (30µg), ceftriaxone (30µg), nitrofurantoin (F) 300µg, amikacin (10µg), and azithromycin (30µg) (Oxoid Ltd.) were used for susceptibility tests. Then, the bacterial isolates were classified as sensitive (S), intermediate (I), or resistance (R) by comparing against the inhibition zone diameter of interpretative standards as indicated in the CLSI guideline.13
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2

Antimicrobial Susceptibility Testing of Pathogens

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Antimicrobial susceptibility testing was performed by the disc diffusion method (Modified Kirby–Bauer technique) using Muller Hinton agar following Clinical Laboratory Standards Institute (CLSI) guidelines.12 In addition to FQ discs (ciprofloxacin (CIP) 5 µg and levofloxacin (LVX) 5 µg), antimicrobial discs included that of carbapenems (Imipenem (IMP) 10 μg, Meropenem (MEM) 10 μg), aminoglycosides (Amikacin (AK) 30 μg, gentamicin (GEN) 10 μg), penicillin/β-lactamase inhibitor (amoxicillin/clavulanic acid (AMC) 20/10 μg), monobactam (Aztreonam (ATM) 30 μg), broad-spectrum cephalosporins (cefoxitin (FOX) 30 μg, ceftazidime (CAZ) 30 μg, cefepime (FEP) 30 μg), trimethoprim-sulfamethoxazole (SXT) 25 μg, and Nitrofurantoin (F) 300 μg, all were obtained from Oxoid Co. (Oxoid Limited, Basingstoke, Hampshire, England). E. coli ATCC®25922TM was used as a quality control strain (American Type Culture Collection Global Bioresource Center, Manassas, VA, USA).12 Multidrug resistance (MDR) phenotype was defined as the non-susceptibility to at least one agent in three or more antimicrobial categories, while extreme drug resistance phenotype (XDR) was defined as the non-susceptibility to at least one agent in all but two or fewer antimicrobial categories (ie, bacterial isolates remain susceptible to only one or two categories).13 (link)
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