Anti mouse igg1 pe

Manufactured by BD

Sourced in United States, Germany

Anti-mouse IgG1-PE is a fluorescently labeled antibody that binds specifically to the IgG1 subclass of mouse immunoglobulins. It is designed for use in flow cytometry and other immunoassay applications to detect and quantify mouse IgG1-positive cells or molecules.

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Lab products found in correlation

Cytofix cytoperm, by BD (2 mentions) Fcs express software, by De Novo Software (2 mentions) Anti 5 methylcytidine, by OriGene (2 mentions) Isotype rat igg1κ, by BD (2 mentions) Facscaliber machine, by BD (2 mentions) Cd4 pe, by BD (1 mentions) Cd8 fitc, by BD (1 mentions) Cd25 fitc, by BD (1 mentions) Cd8 apc, by Thermo Fisher Scientific (1 mentions) Cd3 bv421, by BioLegend (1 mentions) Anti cd16 32, by Thermo Fisher Scientific (1 mentions) Anti slp76, by BD (1 mentions) Anti p plcγ1, by Cell Signaling Technology (1 mentions) Anti pan 14 3 3, by Santa Cruz Biotechnology (1 mentions) Anti 14 3 3ζ, by Santa Cruz Biotechnology (1 mentions) Anti perk1 2, by Merck Group (1 mentions) Anti p p38, by Cell Signaling Technology (1 mentions) Cd4 pe vio770, by Miltenyi Biotec (1 mentions) Anti p jnk, by Cell Signaling Technology (1 mentions) 7 aminoactinomycin d, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

IgG1-PE (61 citations) Mouse IgG1 (48 citations) PE Mouse IgG1 (42 citations) PE‐anti‐IgG1 (4 citations) PE-labeled anti-mouse IgG1 (4 citations) PE‐conjugated anti‐mouse IgG1 (3 citations) Mouse anti (2 citations) Mouse anti-IgG1–PE antibody (2 citations) PE-conjugated rat anti-mouse IgG1 (2 citations) PE-conjugated rat anti–mouse IgG1 Ab (2 citations)

5 protocols using anti mouse igg1 pe

Comprehensive T-cell Signaling Antibody Panel

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Antibodies and reagents used for flow cytometry were: CD8-APC, Fixable viability stain 450, antiCD16/32 (eBiosciences); anti-rabbit-PE (Jackson Immunoresearch); anti-SLP76 (pY128), CD4-PE, CD25-FITC, CD8 FITC and anti-mouse IgG1-PE (BD Biosciences); CD4-PEVio770 (Miltenyi Biotec); CD3-BV421 (Biolegend); anti-pErk1/2 (pT202/pY204) (Cell signaling Technology). The following reagents were used for cell stimulation, immunoblotting or immunoprecipitation: anti-CD3-biontinylated, anti-CD28-biontinylated, anti-CD3ε, anti-CD28 (eBiosciences); streptavidin (Sigma); anti-pErk1/2 (pT202/pY204), anti-pPLCγ1 (pY783), anti-pp38 (pT180/pY182), anti-pJNK (pT183/Y185), anti-pAkt (pS473), anti-pSLP76 (pS376) (Cell Signaling Technology); anti-GADS (Santa Cruz Biotechnology Inc.); rabbit anti-SLP76 or goat-anti-SLP76 (Thermo Fisher Scientific); anti-β-tubulin (Chemicon); for anti-14-3-3 immunoblotting we used a mix of anti-pan 14-3-3 and anti-14-3-3ζ (Santa Cruz, Biotechnology Inc.).

Navas V.H., Cuche C., Alcover A, & Di Bartolo V. (2017). Serine Phosphorylation of SLP76 Is Dispensable for T Cell Development but Modulates Helper T Cell Function. PLoS ONE, 12(1), e0170396.

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Flow Cytometric Measurement of Global DNA Methylation

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Flow cytometric measurement of global methylation was performed as described previously (Delaney et al., 2012 (link)). Briefly, 2.5 × 105 freshly harvested T cells were stained with FITC anti-CD3 Abs and PE-Cy5 anti-CD4, or isotypes, then fixed in Cytofix/Cytoperm (BD) and permeabilized using PBS supplemented with 0.1% saponin, 1% FBS, and 0.1% sodium azide. Cells were treated with RNase A to eliminate the potential for the detection of 5-methylcytidine in tRNA. Cells were treated with anti-5-methylcytidine (Acris Antibodies, San Diego, CA, USA), washed, then incubated with Anti-mouse IgG1-PE or isotype rat-IgG1κ (BD). Flow cytometry was performed immediately or within 24 h of permeabilization using a FACScaliber machine (BD). Results were analyzed with FCS Express software (de novo Software, Los Angeles, CA, USA).

Garg S.K., Delaney C., Toubai T., Ghosh A., Reddy P., Banerjee R, & Yung R. (2014). Aging is associated with increased regulatory T-cell function. Aging Cell, 13(3), 441-448.

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Global DNA Methylation Analysis in T Cells

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Flow cytometric measurement of global methylation was performed as described previously (Delaney et al., 2012 (link)). Briefly, 2.5 × 105 freshly harvested T cells were stained with FITC anti-CD3 Abs and PE-Cy5 anti-CD4, or isotypes, then fixed in Cytofix/Cytoperm (BD) and permeabilized using PBS supplemented with 0.1% saponin, 1% FBS, and 0.1% sodium azide. Cells were treated with RNase A to eliminate the potential for detection of 5-methylcytidine in tRNA. Cells were treated with anti-5-methylcytidine (Acris Antibodies, Germany), washed, then incubated with Anti-mouse IgG1-PE or isotype rat-IgG1κ (BD). Flow cytometry was performed immediately or within 24 h of permeabilization using a FACScaliber machine (BD). Results were analyzed with FCS Express software (De Novo Software).

Garg S.K., Delaney C., Toubai T., Ghosh A., Reddy P., Banerjee R, & Yung R. (2014). Aging is associated with increased regulatory T-cell function. Aging Cell, 13(3), 441-448.

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Splenic B Cell Isolation and CSR

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Isolation of splenic B cells from either IgκAIDyy1^f/f or yy1^f/f mice, deletion of YY1 by recombinant TAT-CRE treatment, LPS plus IL4 treatment, and measurement of CSR to IgG1, were performed as previously described [35 (link)]. Briefly, follicular B cells were purified from mouse spleen with anti-CD23-biotin (eBioscience) and streptavidin microbeads (MACS, Miltenyi Biotec), and conditional YY1 knock-out was performed ex vivo using TAT-CRE enzyme purified from bacteria. Cells were activated ex vivo with 10 μg/ml LPS (Sigma) plus 20 ng/ml IL-4 (Peprotech). Splenic B cells were stained with PE anti-mouse IgG1 (BD Pharmingen) and 7-amino-actinomycin D (Invitrogen) and isotype switching was measured by flow cytometry (dead cells stained with 7AAD were excluded from analysis). Flow cytometry was performed on a FACS Canto II machine, and data was analyzed using FlowJo software.

Zaprazna K., Basu A., Tom N., Jha V., Hodawadekar S., Radova L., Malcikova J., Tichy B., Pospisilova S, & Atchison M.L. (2017). Transcription Factor YY1 Can Control AID-mediated Mutagenesis. European journal of immunology, 48(2), 273-282.

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Immunophenotyping of CD49f- and CD271+CD49f- Cells

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FACS sorted CD49f^- cells or cultured CD271⁺CD49f^- cells were stained with primary antibodies PE-conjugated CD271, PE-conjugated PDGFRB (1.25 μg/ml, mouse IgG1; R&D Systems), PE-conjugated SUSD2, (W5C5 clone, 1:20, mouse IgG1; Biolegend), APC-conjugated CD90 (25 μg/ml, mouse IgG1κ, BD Pharmingen), CD146 (1:2, supernatant, clone CC9, mouse IgG2a; donated by Prof David Haylock, CSIRO, Clayton, Victoria, Australia), CD73 (10 μg/ml, mouse IgG1κ; BD Pharmingen) or CD105 (10 μg/ml, mouse IgG1ƙ; BD Pharmingen). The CD146 samples were incubated with secondary antibody fluorescein isothiocyanate (FITC) conjugated anti-mouse IgG2a (5 μg/ml, clone R19-15; BD Pharmingen). CD73 and CD105 samples were incubated with secondary antibody PE-anti-mouse IgG1 (2 μg/ml, clone A85-1, BD Pharmingen). Isotype matched controls or unlabelled controls were included for each antibody and used to set the electronic gates on the flow cytometer. Cells were then incubated with Sytox Blue and analysed by FACS Canto II analyser (BD Biosciences). FACS data were analysed by FACSDiva software (BD Biosciences).

Letouzey V., Tan K.S., Deane J.A., Ulrich D., Gurung S., Ong Y.R, & Gargett C.E. (2015). Isolation and Characterisation of Mesenchymal Stem/Stromal Cells in the Ovine Endometrium. PLoS ONE, 10(5), e0127531.

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