Ag agcl pellet

Manufactured by World Precision Instruments

The Ag/AgCl pellet is a type of electrode used in various laboratory applications. It consists of a silver (Ag) core coated with silver chloride (AgCl). This electrode serves as a reference electrode, providing a stable and reproducible electrical potential to be used in conjunction with other electrodes for electrochemical measurements and analyses.

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Lab products found in correlation

Matlab 2018, by MathWorks (1 mentions) Micromanipulator, by World Precision Instruments (1 mentions) Syringe pump, by World Precision Instruments (1 mentions)

4 protocols using ag agcl pellet

Flexible MEA Recordings with Customized PCBs

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The BioMAS, an in‐house amplification system,^[⁶¹
^] was used for in vitro recordings with stiff MEA chips with a built‐in 64‐channel headstage to perform electrical recordings. The sampling rate was set to 20 kHz and an Ag/AgCl pellet (World Precision Instruments) was used as reference electrode.
For in vitro and in vivo tissue recordings with flexible MEAs, the ME2100‐System (Multi Channel Systems MCS GmbH, Germany) and a 32 channel headstage (ME2100‐HS32‐M‐3m) were used. The NeuroNexus adapter (ADPT‐NN‐16/32) served to connect the headstage to our customized 16 channel PCBs. The headstage was mounted on a micromanipulator (Luigs & Neumann GmbH, Germany) to enable remotely controlled insertions. The McsMatlabDataTools Matlab toolbox (Multi Channel Systems MCS GmbH, Germany)^[^] was used to import HDF5 files created by the ME2100‐System.

Abu Shihada J., Jung M., Decke S., Koschinski L., Musall S., Rincón Montes V, & Offenhäusser A. (2024). Highly Customizable 3D Microelectrode Arrays for In Vitro and In Vivo Neuronal Tissue Recordings. Advanced Science, 11(13), 2305944.

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Electrical Recording Using BioMAS System

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The BioMAS, an in-house amplification system^{9 (link),42 (link)}, was used with a custom-built 16-channel headstage to perform electrical recordings. The sampling rate was set to 20 kHz and a Ag/AgCl pellet (World Precision Instruments) was used as reference electrode. The spiking activity and LFPs were extracted from the band-pass filtered signal (cutting frequencies of 100 Hz and 3 kHz) and low-pass filtered signal (cutting frequency of 100 Hz) of the raw recordings, respectively. Offline data processing was performed using self-written scripts in Matlab 2018 (The MathWorks Inc.) as reported before^{9 (link)}.

Rincón Montes V., Gehlen J., Ingebrandt S., Mokwa W., Walter P., Müller F, & Offenhäusser A. (2020). Development and in vitro validation of flexible intraretinal probes. Scientific Reports, 10, 19836.

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Electrochemical Flow Cell Protocol

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Working electrodes were positioned in a custom electrochemical flow cell using a micromanipulator (World Precision Instruments, Sarasota, FL). A syringe pump (New Era Pump Systems, Wantagh, NY) supplied a continuous buffer stream (1 mL min⁻¹) across the working and reference electrodes (Ag/AgCl pellet, World Precision Instruments, Inc., Sarasota, FL). Two-second bolus injections of analyte were accomplished with a six-port HPLC valve mounted on a two-position actuator controlled by a digital pneumatic solenoid (Valco Instruments, Houston, TX). The entire apparatus was enclosed in a custom-built, grounded Faraday cage.

Meunier C.J., Mitchell E.C., Roberts J.G., Toups J.V., McCarty G.S, & Sombers L.A. (2018). Electrochemical Selectivity Achieved Using a Double Voltammetric Waveform and Partial Least Squares Regression: Differentiating Endogenous Hydrogen Peroxide Fluctuations from Shifts in pH. Analytical chemistry, 90(3), 1767-1776.

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Cyclic Voltammetry of BBL-P Film

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Cyclic voltammetry was performed in conventional three-electrode cells with Ag/AgCl pellet as the reference electrode (World Precision Instrument) and Pt mesh as the counter electrode. Cyclic voltammetry measurements were performed at room temperature using EG&G Princeton Applied Research, model 273A potentiostat. The BBL-P film prepared as described above served as the working electrode. The supporting electrolyte consisted of 0.1 M KCl in deionized (DI) water, and its pH values were tuned by adding either KOH or HCl such that the final solution always contained at least 0.1 M KCl. The 0.1 M KCl_(aq) electrolyte was purged with a nitrogen stream for at least 15 minutes before any measurements. A stream of N₂ was also constantly flown on top of the electrolyte surface during measurements to minimize oxygen diffusion.

Tran D.K., West S.M., Speck E.M, & Jenekhe S.A. (2024). Observation of super-Nernstian proton-coupled electron transfer and elucidation of nature of charge carriers in a multiredox conjugated polymer. Chemical Science, 15(20), 7623-7642.

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