Abi 7500ht machine

Manufactured by Thermo Fisher Scientific

Sourced in United States, Japan

The ABI 7500HT is a real-time PCR system designed for high-throughput applications. It features a 96-well block format and can be used for a variety of real-time PCR applications.

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Lab products found in correlation

Sybr premix ex taq kit, by Takara Bio (2 mentions) Primescript rt master mix kit, by Takara Bio (2 mentions) Trizol reagent, by Thermo Fisher Scientific (2 mentions) Dnase 1, by Thermo Fisher Scientific (1 mentions) High capacity dna archive kit, by Thermo Fisher Scientific (1 mentions) Sybr green pcr master mix, by Thermo Fisher Scientific (1 mentions) Tri reagent, by Merck Group (1 mentions) Nanodrop 2000, by Thermo Fisher Scientific (1 mentions) Sybr green master mix, by Thermo Fisher Scientific (1 mentions)

4 protocols using abi 7500ht machine

Quantitative RT-PCR Gene Expression Analysis

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Total RNAs were extracted using TRI Reagent (Sigma). Samples were treated with DNase-1 (Ambion) and retro-transcribed using the High Capacity DNA Archive Kit (Applied Biosystems). PCRs were carried out using the SYBR Green PCR Master Mix on an ABI 7500HT machine (Applied Biosystems). Relative quantification was done using the ddCT (Pfaffl) method. Primer sequences are available upon request.

Forte G., Grossi V., Celestini V., Lucisano G., Scardapane M., Varvara D., Patruno M., Bagnulo R., Loconte D., Giunti L., Petracca A., Giglio S., Genuardi M., Pellegrini F., Resta N, & Simone C. (2014). Characterization of the rs2802292 SNP identifies FOXO3A as a modifier locus predicting cancer risk in patients with PJS and PHTS hamartomatous polyposis syndromes. BMC Cancer, 14, 661.

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Quantifying miR-218 and BRD4 Expression

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Total RNA was extracted from BEAS-2B cells or tissues using Trizol reagent (Thermo Fisher Scientific, Waltham, MA, USA). The OD260 nm/OD280 nm of samples value was measured to check RNA purity on Nanodrop 2000 (Applied Biosystems, Foster City, CA, USA). The special primers were used to analyze expression levels of miR-218 and BRD4 with SYBR Green Master Mix (Invitrogen, Carlsbad, CA, USA) under an ABI 7500 HT machine (Applied Biosystems). Glyceraldehyde-3-phosphate dehydrogenase (GAPDH)/endogenous small nuclear RNA U6 was employed as the internal control. The primers were: miR-218 (Forward, 5ʹ-GCCGAGTTGTGCTTGATC-3ʹ; Reverse, 5ʹ-CTCAACTGGTGTCGTGGA-3ʹ);
BRD4 (Forward, 5ʹ-CCCCTCGTGGTGGTGAAG-3ʹ; Reverse, 5ʹ-GCTCGCTGCGGATGATG-3ʹ);
GAPDH (Forward, 5ʹ-TCCCATCACCATCTTCCAGG-3ʹ; Reverse, 5ʹ-GATGACCCTTTTGGCTCCC-3ʹ);
U6 (Forward, 5ʹ-CTCGCTTCGGCAGCACA-3ʹ; Reverse, 5ʹ-AACGCTTCACGAATTTGCGT-3ʹ).

Liu X., Wang J., Luo H., Xu C., Chen X, & Zhang R. (2020). MiR-218 Inhibits CSE-Induced Apoptosis and Inflammation in BEAS-2B by Targeting BRD4. International Journal of Chronic Obstructive Pulmonary Disease, 15, 3407-3416.

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Quantitative Gene Expression Analysis

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Total RNA was isolated with Trizol and reverse-transcribed into cDNA using the PrimeScript RT Master Mix kit (Takara Bio, Japan). The expression of specific genes was quantitated by real time PCR using SYBR Premix Ex Taq kit (Takara Bio, Japan) on an ABI 7500HT machine (Applied Biosystems, USA) with β-actin (human) or 18sRNA (mouse) as internal controls.

Meng J., Feng M., Dong W., Zhu Y., Li Y., Zhang P., Wu L., Li M., Lu Y., Chen H., Liu X., Lu Y., Sun H, & Tong X. (2016). Identification of HNF-4α as a key transcription factor to promote ChREBP expression in response to glucose. Scientific Reports, 6, 23944.

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Quantitative Real-Time PCR Gene Expression Analysis

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Total RNA was isolated with Trizol and reverse‐transcribed into cDNA using the PrimeScript RT Master Mix kit (Takara Bio). The expression of specific genes was quantitated by real‐time PCR using SYBR Premix Ex Taq kit (Takara Bio) on an ABI 7500HT machine (Applied Biosystems) with 18sRNA (rat) as internal control. Primers used in qRT‐PCR are listed in Table 1.

Xing X., Jiang Y., Wang H., Zhang Y., Niu T., Qu Y., Wang C., Wang H, & Liu K. (2020). Identification of novel differentially expressed genes in retinas of STZ‐induced long‐term diabetic rats through RNA sequencing. Molecular Genetics & Genomic Medicine, 8(3), e1115.

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