Constructs encoding MESA fusion proteins were assembled by PCR amplification and standard molecular cloning. MESA constructs were cloned into the adeno-associated virus expression vector plasmid pAAV GFP SN48 , 49 (link), although expression was achieved by transient transfection (not viral packaging). pDSRedExpress2 was included as a transfection control. Source plasmids for MESA components included: pCL-CTIG (Addgene plasmid 14901)50 (link), pRK1043 (Addgene plasmid 8835)28 (link), pBI-MCS-EGFP (Addgene plasmid 16542)31 (link), pBS mCD4 (Addgene plasmid 14613)51 (link), AAV-FLEX-rev-ChR2-tdtomato (Addgene plasmid 18917)52 (link), pEBFP2-Nuc (Addgene plasmid 14893)53 (link), YFP-FKBP (Addgene plasmid 20175)54 (link) and YFP-tagged FRB (YR) (Addgene plasmid 20148)54 (link), pmCherry-C1 (Clontech 632524)27 (link). A complete list of DNA constructs as well as plasmids and primers used for cloning can be found in Supporting Information .
Pcl ctig
Manufactured by Addgene
The PCL-CTIG is a specialized lab equipment used for transfection of mammalian cells. It facilitates the delivery of genetic material, such as plasmids, into cells for various research and experimental applications.
Automatically generated - may contain errors
2 protocols using pcl ctig
1
Engineered MESA Fusion Protein Assembly
2
Assembling MESA Fusion Protein Constructs
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Constructs encoding MESA fusion proteins
were assembled by PCR amplification and standard molecular cloning.
MESA constructs were cloned into the adeno-associated virus expression
vector plasmid pAAV GFP SN,48 (link),49 (link) although expression
was achieved by transient transfection (not viral packaging). pDSRedExpress2
was included as a transfection control. Source plasmids for MESA components
included pCL-CTIG (Addgene plasmid 14901),50 (link) pRK1043 (Addgene plasmid 8835),28 (link) pBI-MCS-EGFP
(Addgene plasmid 16542),31 (link) pBS mCD4 (Addgene
plasmid 14613),51 (link) AAV-FLEX-rev-ChR2-tdtomato
(Addgene plasmid 18917),52 (link) pEBFP2-Nuc (Addgene
plasmid 14893),53 (link) YFP-FKBP (Addgene plasmid
20175)54 (link) and YFP-tagged FRB (YR) (Addgene
plasmid 20148),54 (link) pmCherry-C1 (Clontech
632524).27 (link) A complete list of DNA constructs
as well as plasmids and primers used for cloning can be found inSupporting Information .
were assembled by PCR amplification and standard molecular cloning.
MESA constructs were cloned into the adeno-associated virus expression
vector plasmid pAAV GFP SN,48 (link),49 (link) although expression
was achieved by transient transfection (not viral packaging). pDSRedExpress2
was included as a transfection control. Source plasmids for MESA components
included pCL-CTIG (Addgene plasmid 14901),50 (link) pRK1043 (Addgene plasmid 8835),28 (link) pBI-MCS-EGFP
(Addgene plasmid 16542),31 (link) pBS mCD4 (Addgene
plasmid 14613),51 (link) AAV-FLEX-rev-ChR2-tdtomato
(Addgene plasmid 18917),52 (link) pEBFP2-Nuc (Addgene
plasmid 14893),53 (link) YFP-FKBP (Addgene plasmid
20175)54 (link) and YFP-tagged FRB (YR) (Addgene
plasmid 20148),54 (link) pmCherry-C1 (Clontech
632524).27 (link) A complete list of DNA constructs
as well as plasmids and primers used for cloning can be found in
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