Mcc950 cp 456773 sodium salt

Lipopolysaccharide (LPS; Escherichia coli 0111:B4), ATP, and oxidized ATP (oxATP) were obtained from Sigma (St. Louis, MO, USA), and nigericin was purchased from InvivoGen (San Diego, CA, USA). Ac-YVAD-cmk and MCC950(CP-456773 sodium salt) were purchased from Sigma (St. Louis, MO, USA). Cell Counting Kit-8 (CCK-8) was purchased from Dojindo (Japan). Calcein AM iwas purchased from Invitrogen (Carlsbad, CA, USA) and 1 mg/ml Propidium iodide solution was purchased from Sigma (St. Louis, MO, USA). 1% Crystal violet solution was purchased from Sigma (St. Louis, MO, USA). NLRP3 antibodies and secondary anti-sheep antibody conjugated to horseradish peroxidase (HRP) were purchased from R&D Systems (Minneapolis, MN, USA). Toll-like receptor 4 (TLR4), P2X7, ASC, IL-1β, and caspase-1 donkey anti-rabbit IgG-HRP were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA).

Phenylephrine, acetylcholine, aldosterone, spironolactone and MCC950 (CP-456773 sodium salt) were purchased from Sigma Chemical Co (St. Louis, MO, USA).