Accu check active blood glucose meter

Plasma insulin was measured using a mouse insulin enzyme-linked immunosorbent assay (ELISA) kit (Crystal Chem). Plasma AST and ALT were analyzed using a colorimetric assay kit (BioVision). Plasma FGF-21 was measured using a mouse/rat FGF-21 ELISA kit (R&D systems), and hepatic glucose was determined using a glucose assay kit (Sigma). Hepatic TG was measured by Triglyceride Determination Kit (Sigma). 2-DG uptake of mice was analyzed using a 2-DG uptake measurement kit (Cosmo Bio Co.). Blood glucose levels were determined from tail vein blood using an Accu-Check Active blood glucose meter (Roche).

The whole blood glucose levels were tested using the Accu-Check Active Blood Glucose Meter (Roche, Shanghai, China). The serum was promptly centrifuged at 3500 rpm for 10 min at 4 °C and then stored at 4 °C. The levels of lactic acid (LA), lactate dehydrogenase (LDH), BUN, creatine kinase (CK), triglyceride (TG), total cholesterol (TC), alanine aminotransferase (ALT), and aspartate aminotransferase (AST) were determined using an auto-analyzer (AU680, Beckman Coulter, Brea, CA, USA). The levels of MDA, SOD, and GSH-Px were determined using a commercially available enzyme-linked immunosorbent assay (ELISA) kit according to the manufacturer’s protocols.