Porcine specific elisa kit

Manufactured by R&D Systems

Sourced in United States

Porcine-specific ELISA kits are laboratory equipment designed to detect and quantify target analytes in porcine (pig) samples using the enzyme-linked immunosorbent assay (ELISA) technique. These kits provide a standardized and reliable method for researchers and scientists working with porcine models.

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Lab products found in correlation

Microplate reader, by Agilent Technologies (1 mentions) Tri reagent protocol, by Merck Group (1 mentions) Taqman gene expression assay protocol, by Thermo Fisher Scientific (1 mentions) Quantitect reverse transcription kit, by Qiagen (1 mentions) Amplex red hydrogen peroxide assay kit, by Thermo Fisher Scientific (1 mentions) Au5811 analyzer, by Beckman Coulter (1 mentions)

7 protocols using porcine specific elisa kit

Porcine Colostrum Cytokine Profile

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The composition of colostrum was measured using an automatic milk analyzer (Milk-Yway-CP2, Beijing, China). The concentrations of cytokines including interleuckin-6 (IL6), interleukin-1β (IL1β) and tumor necrosis factor-α (TNFα) were measured using the porcine-specific ELISA kits (R&D Systems, Minneapolis, MN, USA) according to the manufacturers’ instructions [19 (link),20 (link),21 (link)].

Zou T., Wei W., Cao S., Zhang H, & Liu J. (2020). Effects of Dietary Fat Sources during Late Gestation on Colostrum Quality and Mammary Gland Inflammation in Lipopolysaccharide-Challenged Sows. Animals : an Open Access Journal from MDPI, 10(2), 319.

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Porcine Thermal Injury Wound Healing

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The therapies or controls were re-applied during the dressing changes on postoperative days 2 and 5. On day 10, the dressings were removed and the animals euthanized after assessing the wounds. Blood draws were performed before and after thermal injury and during the dressing changes. Routine laboratory parameters (haemoglobin, white blood cell count) were determined by the central laboratory of the University of Kaposvar. Serum levels of IL-1b, IL-6, and TNF-alpha were determined using commercially available porcine-specific ELISA kits (R&D Systems, Minneapolis, MN, USA).

Hacker S., Mittermayr R., Nickl S., Haider T., Lebherz-Eichinger D., Beer L., Mitterbauer A., Leiss H., Zimmermann M., Schweiger T., Keibl C., Hofbauer H., Gabriel C., Pavone-Gyöngyösi M., Redl H., Tschachler E., Mildner M, & Ankersmit H.J. (2016). Paracrine Factors from Irradiated Peripheral Blood Mononuclear Cells Improve Skin Regeneration and Angiogenesis in a Porcine Burn Model. Scientific Reports, 6, 25168.

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Porcine Inflammatory Cytokine Assay

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Serum collected at eight-week feeding was assayed for interleukin-1β (IL-1β), interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α) using porcine-specific ELISA kits (R&D Systems, Minneapolis, MN) according to the manufacturer's instructions.

Yamada S., Kawaguchi H., Yamada T., Guo X., Matsuo K., Hamada T., Miura N., Tasaki T, & Tanimoto A. (2017). Cholic Acid Enhances Visceral Adiposity, Atherosclerosis and Nonalcoholic Fatty Liver Disease in Microminipigs. Journal of Atherosclerosis and Thrombosis, 24(11), 1150-1166.

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Porcine Serum Cytokine Quantification

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Porcine-specific ELISA kits (R&D Systems, Minneapolis, MN, USA) for IL-6 and TNF-α were used to quantify serum cytokines and assays were conducted following manufacturer’s protocol. Serum samples were left undiluted.

Radlowski E.C., Wang M., Monaco M.H., Comstock S.S, & Donovan S.M. (2021). Combination-Feeding Causes Differences in Aspects of Systemic and Mucosal Immune Cell Phenotypes and Functions Compared to Exclusive Sow-Rearing or Formula-Feeding in Piglets. Nutrients, 13(4), 1097.

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Quantifying Serum LPS and DAO Levels

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A porcine-specific ELISA kit (R&D System, Minneapolis, MN) and a microplate reader (BioTek Instruments, Winooski, VT) were used to quantify the serum LPS concentration, in accordance with the manufacturer's protocol. The serum DAO concentration was measured using a commercial kit produced by Nanjing Jiancheng Bioengineering Institute (Jiangsu, China), and quantified by using a UV-vis spectrophotometer (UV1100, Shanghai, China).

Diao H., Xiao Y., Yan H.L., Yu B., He J., Zheng P., Yu J., Mao X.B, & Chen D.W. (2021). Effects of Early Transplantation of the Faecal Microbiota from Tibetan Pigs on the Gut Development of DSS-Challenged Piglets. BioMed Research International, 2021, 9823969.

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Porcine Microglia Activation Assay

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CD11b⁺ cells were diluted (~5×10⁵ cells/mL) in DMEM containing 10% heat-inactivated FBS, 1% penicillin-streptomycin, and 10 ng/mL recombinant porcine GM-CSF, and plated in duplicate in 24-well plates (~5×10⁵ cells/well). After 24 h at 37°C 5% CO2, DMEM, LPS (1 ng/mL DMEM), or Poly I:C (1 μg/mL DMEM) was added to wells. Following 8 h incubation, medium was collected and assayed for TNFα using a commercially available porcine-specific ELISA kit (R&D systems). Conditioned supernatants were assayed for lactate dehydrogenase using a commercially available detection kit (Roche Diagnostics Corporation) following the manufacturer’s instructions to verify treatments were not cytotoxic. Total RNA from microglia was isolated using TRI Reagent protocol (Sigma), and cDNA was synthesized using a QuantiTect Reverse Transcription Kit (QIAGEN). The real-time PCR was performed using the Applied Biosystems Taqman Gene Expression Assay Protocol. Genes of interest (Table S1) were compared with and expressed as fold change relative to the reference gene (GAPDH, Ss03374854_g1, Applied Biosystem).

Ji P., Schachtschneider K.M., Schook L.B., Walker F.R, & Johnson R.W. (2016). Peripheral Viral Infection Induced Microglial Sensome Genes and Enhanced Microglial Cell Activity in the Hippocampus of Neonatal Piglets. Brain, behavior, and immunity, 54, 243-251.

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Plasma Metabolite and Hormone Analysis

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The concentrations of triglyceride (TG) and nonesterified fatty acids (NEFA) in plasma samples were determined by using Beckman AU5811 analyzer (Beckman-Coulter, Inc.250S.Kraemer Boulevard Brea, CA, USA) as previously reported [8 (link)]. A commercially available porcine specific ELISA kit (RD SYSTEMS, Minneapolis, MN, USA) and an Amplex Red hydrogen peroxide assay kit (Invitrogen, Carlsbad, CA, USA) were utilized to measure plasma leptin and hydrogen peroxide (H₂O₂) content, respectively, according to the manufacturer’s instructions as previously described [5 (link),17 (link)]. All blood samples were determined in duplicate in single assay. The minimum detection limit was 3.2 pg/dL, 0.1 pmol/L, 0.2 ng/mL, and 0.2 pmol/L for TG, NEFA, Leptin, and H₂O₂, respectively. For Leptin and H₂O₂ analysis, within-assay coefficient of variation (CV) was acceptable when less than 5%.

Tian L., Huang J., Wen A, & Yan P. (2020). Impaired Mitochondrial Function Results from Oxidative Stress in the Full-Term Placenta of Sows with Excessive Back-Fat. Animals : an Open Access Journal from MDPI, 10(2), 360.

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