Morphine (Sigma; Cat No: 25-27-2) and 5-aza-2′-deoxycytidine (5-aza-dC; Sigma; Cat No: 2353-33-5) were dissolved in saline; saline was set as the control. Suberoylanilide hydroxamic acid (SAHA; Selleck Chemicals, Houston, TX, United States; Cat No: S1047) was dissolved in 5% dimethyl sulfoxide (DMSO; Sigma; Cat No: D2650-100 mL); 5% DMSO was set as the control. Morphine was subcutaneously given with a dose of 1.5 mg per kg of body weight. The use of 5-aza-dC was continued for three consecutive days at 5 μg/5 μL daily, 30 min before surgery. SAHA was continued for 7 consecutive days, at a dose of 50 mg/kg in a volume of 5 μL, 30 min before surgery. All drug doses are based on preliminary experiments (Zhou et al., 2014 (link); Cao et al., 2015 (link); Garriga et al., 2018 (link)), and did not alter the nociceptive response per se. Intrathecal injections were performed manually between L5 and L6 spinal segments, through the theca of the spinal cord into the subarachnoid space (Hylden and Wilcox, 1980 (link)). On entry of the needle, a quick flicking motion of the mouse’s tail or leg was noticed. For anesthesia, sodium pentobarbital and isoflurane (Sigma; Cat No: 26675-46-7) were used.
Suberoylanilide hydroxamic acid
Manufactured by Selleck Chemicals
Sourced in United States
Suberoylanilide hydroxamic acid is a synthetic organic compound. It is a histone deacetylase (HDAC) inhibitor.
Automatically generated - may contain errors
Lab products found in correlation
Morphine, by Merck Group (1 mentions)
Isoflurane, by Merck Group (1 mentions)
5 aza 2 deoxycytidine 5 aza dc, by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Ribavirin, by Selleck Chemicals (1 mentions)
Trichostatin a tsa, by Selleck Chemicals (1 mentions)
Pegfr y1173, by Cell Signaling Technology (1 mentions)
Lbh589, by Novartis (1 mentions)
Transmission electron microscope, by Nikon (1 mentions)
Beclin1 sirna, by Santa Cruz Biotechnology (1 mentions)
4 protocols using suberoylanilide hydroxamic acid
1
Pharmacological Modulation of Nociception
2
Treating Viral Myocarditis in Mice
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BALB/c mice in each group were infected by an intraperitoneal injection with 1 × 103 TCID50 CVB3 on day 0. To examine the therapeutic effects of suberoylanilide hydroxamic acid (SAHA; Selleck), the solution of SAHA in 2-hydroxypropyl-β-cyclodextrin (HOP-β-CD; Sigma-Aldrich) was prepared as previously described (22 (link)). Mice were orally administered daily with 50 mg/kg of body weight of SAHA, starting from the day of virus inoculation. In addition, trichostatin A (TSA; Selleck) was dissolved in dimethyl sulfoxide (DMSO) and injected intraperitoneally into mice at 0.5 mg/kg/day. Ribavirin (Selleck) was dissolved in phosphate-buffered saline (PBS) and injected intraperitoneally into mice at 100 mg/kg/day.
3
SAHA and LBH589 effects on EGFR
4
Investigating Autophagy in MYCN-Amplified Cells
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SK‐N‐SH and QDDQ‐NM cells, in which MYCN was amplified and expressed at normal levels, respectively, were supplied by the Cell Bank of the Chinese Academy of Sciences (Beijing, China). The methyl thiazolyl tetrazolium (MTT), monodansylcadaverine (MDC) and Alexa Fluor 430‐A were purchased from Sigma (San Francisco, CA, USA). The microplate reader and MetaMorph offline 7.7.8.0 software packagewere purchased from Bio‐Rad (Hercules, CA, USA). The transmission electron microscope was purchased from NIKON (TKY, Japan). The Beclin 1 siRNA and TRP14 siRNA were both purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA), and the suberoylanilide hydroxamic acid was purchased from Selleck Chemicals (Houston, TX, USA).
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