Tissue lysates from mouse cerebral cortex and spleen were prepared and stored at −80°C until use as described previously (Miyake et al. 2016 (link)). Lysates were then separated using sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (PAGE), transferred to a polyvinylidene difluoride (PVDF) membrane (Bio-Rad, Hercules) and immunoblotted with primary antibodies using a SNAP i.d 2.0 Protein Detection System (Merck Millipore) as previously described (Nakahama et al. 2018 (link)). The primary antibodies used were as follows: mouse monoclonal anti-ADAR1 antibody (15.8.6; Santa Cruz Biotechnology), mouse monoclonal anti-ADAR2 antibody (1.3.1; Santa Cruz Biotechnology), and mouse monoclonal anti-GAPDH (M171-3; MBL).
Mouse monoclonal anti adar2 antibody
Manufactured by Santa Cruz Biotechnology
Mouse monoclonal anti-ADAR2 antibody is a laboratory tool used to detect and study the ADAR2 protein in biological samples. ADAR2 is an enzyme involved in the process of RNA editing.
Automatically generated - may contain errors
Lab products found in correlation
Snap i d 2.0 protein detection system, by Merck Group (1 mentions)
Mouse monoclonal anti adar1 antibody 15.8.6, by Santa Cruz Biotechnology (1 mentions)
Pvdf membrane, by Bio-Rad (1 mentions)
Mouse monoclonal anti gfp antibody, by Santa Cruz Biotechnology (1 mentions)
Pierce ecl western blotting substrate, by Thermo Fisher Scientific (1 mentions)
Polyvinylidene difluoride membrane, by Bio-Rad (1 mentions)
2 protocols using mouse monoclonal anti adar2 antibody
1
Immunoblotting of ADAR Enzymes in Mouse Tissues
2
Western Blot Analysis of RNA-Editing Enzymes
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Each lysate was separated by SDS-polyacrylamide gel electrophoresis and transferred to polyvinylidene difluoride membranes (Bio-Rad). Immunoblotting was performed by using the following primary antibodies: mouse monoclonal anti-ADAR1 antibody (catalog no.: 15.8.6; Santa Cruz Biotechnology), mouse monoclonal anti-ADAR2 antibody (catalog no.: 1.3.1; Santa Cruz Biotechnology), rabbit polyclonal anti-AZIN1 antibody (catalog no.: 11548-1-AP; Proteintech), mouse monoclonal anti-GFP antibody (catalog no.: GF200; Santa Cruz Biotechnology), rabbit polyclonal anti-Halo Tag antibody (catalog no.: G 928A; Promega), and mouse monoclonal anti-GAPDH antibody (catalog no.: M171-3; MBL). Chemiluminescence was detected by using ImmunoStar Zeta (Fujifilm) or Pierce ECL Western Blotting Substrate (Thermo Fisher Scientific).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!