Starscript first strand cdna synthesis kit

Total RNA was extracted using the RNeasy Mini kit (Qiagen) following the manufacturer’s protocol and then subjected to reverse transcription using the StarScript first strand cDNA synthesis kit (Transgen Biotech, Beijing, China). Real-time PCR was performed using SYBR Select Master Mix (Applied Biosystems) on an ABI PRISM 7500 Sequence Detector (Applied Biosystems). GAPDH served as an internal control for normalization [40 (link)].
The primers for RT-qPCR are listed as below:
S100A13 forward:
5’-TCCTAATGGCAGCAGAACCACTGA-3’ and
reverse:
5’- TTCTTCCTGATTTCCTTGGCCAGC-3’
IL-6 forward:
5’- TACCCCCAGGAGAAGATTCC -3’ and
reverse:
5’- TTTTCTGCCAGTGCCTCTTT -3’
IL-8 forward:
5’- TAGCAAAATTGAGGCCAAGG -3’ and
reverse:
5’- AAACCAAGGCACAGTGGAAC -3’
CXCL2 forward:
5’- GCCCAACGCACCGAATAGT-3’ and
reverse:
5’- CGCTGCCCATCATCATGAC-3’
CCL2 forward:
5’- AGTTCTTGCCGCCCTTCT -3’ and
reverse:
5’- GTGACTGGGGCATTGATTG -3’
IL-1β forward:
5’- GGGCCTCAAGGAAAAGAATC -3’ and
reverse:
5’- TTCTGCTTGAGAGGTGCTGA -3’
MMP3 forward:
5’- GCAGTTTGCTCAGCCTATCC -3’ and
reverse:
5’- GAGTGTCGGAGTCCAGCTTC -3’
IL-7 forward:
5’- CGCAAGTTGAGGCAATTTCT -3’ and
reverse: 5’- CTCTTTGTTGGTTGGGCTTC -3’
CXCL1 forward:
5’- AGGGAATTCACCCCAAGAAC -3’ and
reverse:
5’- TGGATTTGTCACTGTTCAGCA -3’
GAPDH forward:
5’- CGACCACTTTGTCAAGCTCA -3’ and
reverse:
5’- AGGGGTCTACATGGCAACTG -3’

Total RNA was isolated from hippocampus with Trizol reagent (Invitrogen, Grand Island, NY, USA) and then subjected to reverse transcription using the StarScript first strand cDNA synthesis kit (Transgen Biotech, Beijing, China). Real-time PCR was performed in triplicate using the SYBR Green PCR Master Mix (Applied Biosystems). The β-actin gene served as an endogenous control for normalization. Relative expression levels of different genes were calculated by the 2^-ΔΔCt method, and the histogram for fold comparison of different samples was generated by GraphPad Prism 5 (Roche, Switzerland). Experiments were carried out in triplicate three times. The sequences of primers for qRT-PCR were summarized in Supplementary Table 1.

Total RNA was extracted using the RNeasy Mini kit (Qiagen) following the manufacturer’s protocol and then subjected to reverse transcription using the StarScript first strand cDNA synthesis kit (Transgen Biotech, Beijing, China). Real-time PCR was performed using SYBR Select Master Mix (Applied Biosystems) on an ABI PRISM 7500 Sequence Detector (Applied Biosystems). GAPDH was served as an internal control for normalization. Results are representative of three independent experiments, and values are the mean ± SD (error bars). P < 0.05 (*) or P < 0.01 (**).
The primers for RT-qPCR are listed as below:
KAT7 forward: 5′-GAATGCAAGGTGAGAGCACA-3′;
KAT7 reverse: 5′-CCGTGTGTTCCCATAGGTCT-3′;
GAPDH forward: 5′-CCATGGGGAAGGTGAAGGTC-3′;
GAPDH reverse: 5′-GAAGGGGTCATTGATGGCAAC-3′.