Proton relaxation measurement of retrograded rice cake was carried out using a low-resolution (20 MHz) 1H NMR spectrometer (Minispec mq pulsed NMR, Bruker, Whitney, UK). The samples were placed in 16 mm diameter NMR glass tubes and covered with a cap to avoid moisture loss. All measurements were performed at 40 °C. The Carr–Purcell–Meiboom–Gill (CPMG) pulse sequence was used for acquisition of the free induction decay (FID) data for the transverse relaxation time (T2). The experimental parameters were set appropriately to maximize the signal-to-noise ratio and to cover the entire relaxation range as completely as possible. The FID(s) obtained from the CPMG pulse sequence were analyzed using a mono-exponential model: 90x − (t − 180y − τ − echo)n. Pulse lengths with 90° and 180° pulses were separated by 20 μs, a relaxation delay of 6 s, and a τ of 0.7, and 120 data points measurements were performed using RI WinDXP software (version 1.2.2., Resonance Instruments Ltd., Oxfordshire, UK).
Minispec mq
Manufactured by Bruker
Sourced in United Kingdom, Germany, United States
The Minispec mq is a compact and versatile nuclear magnetic resonance (NMR) spectrometer designed for various analytical applications. It provides reliable and precise measurements without the need for sample preparation or destruction.
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5 protocols using minispec mq
1
Retrograded Rice Cake Proton Relaxation
2
Magnetic Relaxivity of Liposomal Gadolinium
3
Non-Invasive Body Composition Analysis
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The following day of the last stress session (between 9 AM and 1 PM), fat and lean body mass were determined. Measurements were carried out with conscious mice in a 3-minute trial using the Minispec mq benchtop NMR spectrometer (Bruker Instruments, Rheinstetten, Germany).24 (link),25 (link)
4
NMR Relaxometry of Biomaterials
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NMR measurements were carried out on a Bruker Minispec mq (mq20, Bruker, Billerica, MA, USA) 60 operating at 60 MHz proton resonance frequency corresponding to a magnetic field of 1.41 T. π/2 pulse length is 2.8 µs, and the typical value of dead time is 7 µs. The temperature of the magnet, the probe temperature and samples (around 20 mg) were thermalized to 37 °C. Before each measurement, a duration of 30 min was allowed for temperature equilibration of the samples.
Carr–Purcell–Meiboom–Gill sequence (CPMG) was used for the T2 study. Spectra were acquired setting 600 echoes, with an accumulation of 1024 scans spaced out by a recycle delay of 3 s. Echo spacing of 40 µs. Signal treatment was operated using Minispec software giving discrete values of relaxation time and corresponding intensity.
Carr–Purcell–Meiboom–Gill sequence (CPMG) was used for the T2 study. Spectra were acquired setting 600 echoes, with an accumulation of 1024 scans spaced out by a recycle delay of 3 s. Echo spacing of 40 µs. Signal treatment was operated using Minispec software giving discrete values of relaxation time and corresponding intensity.
5
Body Composition Analysis in Mice
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