Western blot analysis was performed as described previously.30 (link) Antibodies against β-actin (sc-69879, 1:5,000), MTA2 (sc-55566, 1:1,000), and KLK10 (sc-100551, 1:1,000) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Antibodies against Sp1 (07645, 1:1,000) and horseradish peroxidase (HRP)-conjugated anti-mouse (AP124P, 1:10,000) were purchased from Merck Millipore (Burlington, MA, USA).
Horseradish peroxidase hrp conjugated anti mouse ap124p
Manufactured by Merck Group
Sourced in United States
Horseradish peroxidase (HRP)-conjugated anti-mouse (AP124P) is a laboratory reagent used for immunodetection techniques. It consists of an anti-mouse antibody conjugated to the enzyme horseradish peroxidase. This conjugate can be used to detect and visualize target proteins in various immunoassays, such as Western blotting, ELISA, and immunohistochemistry.
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2 protocols using horseradish peroxidase hrp conjugated anti mouse ap124p
1
Western Blot Analysis of Protein Targets
2
Cell Viability and Protein Expression
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CCK8 kit was purchased from Sigma-Aldrich (St. Louis, MO, USA). The primary antibodies against β-actin (sc-69879, 1:5000) was purchased from Santa Cruz Biotechnology (Santa Cruz, Dallas, TX, USA). Antibodies against PROK2 (24906-1-AP, 1:1000) was purchased from BioTools Co. Ltd. (Taipei, Taiwan). Antibodies against MMP15 (130522, 1:500) was obtained from Novus Biologicals (Briarwood, CO, USA) and horseradish peroxidase (HRP)-conjugated anti-mouse (AP124P, 1:10,000) were purchased from Merck Millipore (Burlington, MA, USA).
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