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D 001206 14

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The D-001206-14 is a laboratory equipment product. It is a core component used in various scientific applications. The specific function and intended use of this product is not available based on the information provided.

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Lab products found in correlation

Lipofectamine rnaimax transfection reagent, by Thermo Fisher Scientific (1 mentions) I fect, by Neuromics (1 mentions)

2 protocols using d 001206 14

1

Knockdown of Cellular and Viral Genes

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HFFs were transfected with 200 nmol/l siRNA using Lipofectamine RNAiMAX transfection reagent (Life Technologies). For knockdown of the cellular genes targeting the cellular genes PDGFRα and NRP2 premixed pools of four siRNAs were used (M-003162-04 and M-017721-01; Thermo Fisher Scientific). For knockdown of the viral genes UL74 and UL128, two individual siRNAs per gene were designed and purchased from Sigma-Aldrich: UL74 was targeted by 5′-CGAACAAGGCUGCGGUAAU(dT)(dT)-3′ and 5′-GGUCCCAUUCGAAACGAUA(dT)(dT)-3′, UL128 was targeted by 5′-GCGGCAAAGUGAACGACAA(dT)(dT)-3′ and 5′-CUGCUACAGUCCCGAGAAA(dT)(dT)-3′. To control for unspecific effects of siRNA transfection, a pool of non-targeting (NT) siRNAs was included (D-001206-14; Thermo Fisher Scientific).
Weiler N., Paal C., Adams K., Calcaterra C., Fischer D., Stanton R.J., Stöhr D., Laib Sampaio K, & Sinzger C. (2021). Role of Envelope Glycoprotein Complexes in Cell-Associated Spread of Human Cytomegalovirus. Viruses, 13(4), 614.
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2

Noradrenaline Depletion and GLT-1 Knockdown in Locus Coeruleus

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For depletion of noradrenaline in the LC, dopamine-β-hydroxylase antibody conjugated to saporin (DβH-saporin, Sigma-Aldrich Co., St. Louis, MO) or its negative control IgG-saporin (Sigma-Aldrich Co.) was dissolved with saline to achieve a final concentration of 0.25 μg/0.5 μl and injected into the LC through the guide cannula at 2 weeks prior to the microdialysis experiment. For knock-down of GLT-1 in the LC, a small interfering RNA (siRNA) mixture for rat GLT-1 (SMARTpool #M-091209-02, Thermo Fisher Scientific Inc., Pittsburgh, PA) or a non-targeting siRNA pool (#D-001206-14, Thermo Fisher Scientific Inc.) was dissolved in double distilled water, diluted with the transfection reagent (i-Fect; Neuromics, Edina, MN) to achieve a final concentration of 8.3 pmol/0.5 μl, and injected through the guide cannula for 5 consecutive days.
Suto T., Severino A.L., Eisenach J.C, & Hayashida K.I. (2014). Gabapentin increases extracellular glutamatergic level in the locus coeruleus via astroglial glutamate transporter-dependent mechanisms. Neuropharmacology, 81, 95-100.
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