Sodium citrate

Manufactured by Sangon

Sourced in China

Sodium citrate is a chemical compound commonly used as a laboratory reagent. It is a salt of citric acid, with the chemical formula Na₃C₆H₅O₇. Sodium citrate is a white, crystalline powder that is soluble in water.

Automatically generated - may contain errors

Lab products found in correlation

5 protocols using sodium citrate

PSPH Immunohistochemistry Staining Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

4 μm thick FFPE NPC slides were used to perform IHC staining. Firstly, the tumor slides were deparaffinized. Antigen retrieval procedure was then performed in a pressure cooker in 1 mM sodium citrate (Sangon Biotech, Shanghai, China) for 1.5 minutes. PSPH rabbit polyclonal (Proteintech, Wuhan, China) was diluted with SigalStain antibody diluent (Cell Signaling Technology, Danvers, MA, USA) at 1:400 for 1 h. Universal secondary antibody (Gene Tech, Shanghai, China) was applied for 15 minutes. Subsequently, diaminobenzidine was used as chromogens and slides were counterstained with haematoxylin before mounting. PSPH IHC was scored according to the scoring criteria including intensity and percentage as follows: 0, no staining; 1+, faint cytoplasmic reactivity without any background staining; 2+, moderate cytoplasmic reactivity; and 3+, granular cytoplasmic reactivity of strong intensity; 0, 0-25%; 1, 25%-50%; 2, 50%-75% [17 (link)].

Wang J., Xie G.F., He Y., Deng L., Long Y.K., Yang X.H., Ma J.J., Gong R., Cen W.J., Ye Z.L., Zeng Y.X., Wang H.Y, & Shao J.Y. (2019). Interfering Expression of Chimeric Transcript SEPT7P2-PSPH Promotes Cell Proliferation in Patients with Nasopharyngeal Carcinoma. Journal of Oncology, 2019, 1654724.

+ Open protocol

+ Expand

Fermentation of Soybean Residue with Yeast and LAB

Check if the same lab product or an alternative is used in the 5 most similar protocols

Fresh soybean residue was provided by Kangle Tofu Workshop (Changchun, China). Soybean residue should be dried for 24 h at 55 °C before reuse. Saccharomyces cerevisiae strain BNCC337309 was obtained from the BeNa Culture Collection (Beijing, China). The KC205 LAB strain was isolated and screened from homemade pickled cabbage juice by the food microbiology team of the Agro-product Process Institute of the Jilin Academy of Agricultural Sciences, and has the characteristics of strong acid production and acid resistance.
High temperature-resistant α -amylase (2 × 10⁴ U/mL), papain (800 U/mg), and amyloglucosidase (1 × 10⁵ U/mL), were purchased from Shanghai Yuanye Bio-Technology Co., Ltd. (Shanghai, China). Peptone, yeast extract fermentation, glucose, NaCl, beef extract, anhydrous sodium acetate, sodium citrate, K₂HPO₄, MgSO₄, MnSO₄ and Tween 80 were all purchased from Sangon Biotech Co., Ltd. (Shanghai, China). All chemicals were of analytical grade or better.

Xu X., Zhang X., Sun M., Li D., Hua M., Miao X., Su Y., Chi Y., Wang J, & Niu H. (2023). Optimization of Mixed Fermentation Conditions of Dietary Fiber from Soybean Residue and the Effect on Structure, Properties and Potential Biological Activity of Dietary Fiber from Soybean Residue. Molecules, 28(3), 1322.

+ Open protocol

+ Expand

Immunohistochemical Analysis of PP2A Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols

After tissue sections were deparaffinized, rehydrated with a graded ethanol series, incubated with 0.1 mol/L sodium citrate (Sangon, Shanghai, China) for 20 minutes and blocked with 10% BSA (Sangon), the slides were incubated with anti-PP2A alpha+beta antibody (Abcam, Cambridge, UK at 4 °C overnight. After washing, the slides were labeled with an HRP-conjugated secondary antibody (Sangon) at room temperature for 1 hour. Positive staining was visualized with a DAB substrate solution (Sangon), and counterstaining was performed with hematoxylin. According to the ratio of positive-staining cells, 0–5% scored 0, 6–35% scored 1, 36–70% scored 2, and more than 70% scored 3, and according to the staining intensity, no staining was scored as 0, weak staining was 1, moderate staining was 2, and strong staining was 3. The final score was determined using the score for the percent of positive cells × the staining intensity score as follows: “–” for a score of 0–1; “+” for a score of 2–3; “+ +” for a score of 4–6; and “+ + +” for a score of > 6. Low expression was defined as a total score < 4, and high expression was defined as a total score = 4. These scores were determined independently by two senior pathologists in a blinded manner.

Liu L., Wang H., Cui J., Zhang Q., Zhang W., Xu W., Lu H., Liu S., Shen S., Fang F., Li L., Yang W., Zhuang Z, & Li J. (2018). Inhibition of Protein Phosphatase 2A Sensitizes Mucoepidermoid Carcinoma to Chemotherapy via the PI3K-AKT Pathway in Response to Insulin Stimulus. Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 50(1), 317-331.

+ Open protocol

+ Expand

Immunohistochemical Staining of MPC2 and Ki67

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

IHC staining was conducted following a standard procedure as previously described [16 (link)]. Tissues were embedded with paraffin and incised into 4 μm thick sections. Dewaxing the sections by xylene and hydrating by alcohol were then performed, followed by antigen retrieval via sodium citrate (Sangon Biotech, China) and endogenous peroxidase blocking by 0.3% H₂O₂. Bovine serum albumin (BSA, Sangon Biotech) was used to block nonspecific sites, and MPC2 (1 : 100, ab236584, Abcam) and Ki67 (1 : 100, 27309-1-AP, Proteintech, China) were used to incubate the slices for 8 hrs then with secondary antibody (1 : 100, SA00001-2, Proteintech, China) for 60 minutes. The DAB Substrate kit (# 8059S, CST) was used for visualization; then, nuclei were stained with hematoxylin. Further dehydration was performed with alcohol and sealing with neutral resin. IHC stainings were qualified using a grade scoring method, and it was scored from 1 to 4 as no, weak, moderate, and strong staining, respectively [17 (link), 18 (link)]. Besides, samples were divided into two groups according to their MPC2 staining: the low level group (scores 1 and 2) and the high level group (scores 2 and 3).

Kuerbanjiang M., Gu L., Xu C., Xu W.T., Wen S., Xue H, & Xu Q. (2021). Decreased Expression of MPC2 Contributes to Aerobic Glycolysis and Colorectal Cancer Proliferation by Activating mTOR Pathway. Journal of Immunology Research, 2021, 6618837.

+ Open protocol

+ Expand

Solvothermal Synthesis of ZnO Nanoparticles

Check if the same lab product or an alternative is used in the 5 most similar protocols

Zinc acetate (Zn(CH₃COO)₂·2H₂O), sodium citrate (Na₃C₆H₅O₇·2H₂O), sodium hydroxide (NaOH), and ethanol (AR) were purchased from Sangon Biotech (China, www.sangon.com). ZnO was synthesized by a simple one-step method. A total of 0.2195 g of zinc acetate and 0.2941 g of sodium citrate were dissolved in 40 mL of deionized water and magnetically stirred for 10 min to form a clear solution. The above mixed solution was used as a precursor solution. Then, a certain amount of 3 mol/L NaOH solution was added dropwise to the precursor solution, and the PH values of the solutions were adjusted to be 9, 10 and 11. The above mixed solution was transferred to a 100 mL autoclave, sealed, and placed in a constant temperature drying oven at 120 °C for 8 h. It was cooled to room temperature, washed several times with deionized water and absolute ethanol, and placed in a constant temperature drying oven at 60 °C to be dried. We named the samples synthesized under the above three different PH values ZnO-1, ZnO-2, and ZnO-3.

Yu S., Zhang H., Zhang J, & Li Z. (2019). Effects of pH on High-Performance ZnO Resistive Humidity Sensors Using One-Step Synthesis. Sensors (Basel, Switzerland), 19(23), 5267.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!