Agilent 8453 uv visible spectrometer

Structural conformation of synthesized MNPs was verified using Bruker GADDS/D8 X-ray diffraction system with Apex Smart CCD Detector and Mo direct-drive rotating anode (50 kV; 20 mA). Diffraction patterns were analyzed and indexed using ICDD PDF 2015 database and Match software. Further, to confirm the elemental composition of MNPs, energy dispersive spectroscopy (EDS) was conducted in scanning electron microscopy (JEOL JSM 5900LV) at 15 kV and working distance of 10 mm.
The hydrodynamic radius and size distribution of MNPs were analyzed using dynamic laser scattering (DLS) (90 Plus particle size analyzer, Brookhaven Instruments, USA) at room temperature. Further, to examine the original crystal size, transmission electron microscopy (TEM) analysis was performed with the JEOL 1010 Transmission Electron microscope operated at 100 kV. The magnetization curve of MNPs was measured using vibrating sample magnetometer (VSM-3, Toei Kogyo, Tokyo, Japan) equipped with an electromagnet (TEM-WFR7, Toei Kogyo, Tokyo, Japan) and a gaussmeter (Model 421, Lake Shore Cryotronics, Inc.). The measurement was conducted at room temperature with a maximum field of 780 kA/m.
The Agilent 8453 UV-Visible Spectrometer with Quartz-1 cm path length was used for evaluating absorbance of MNPs from 200 to 1000 nm wavelength.

General chemicals were purchased from TCI (Tokyo Chemical Industry, Tokyo, Japan) and were used without further purification. All antibiotics used were purchased from Sigma-Aldrich (St. Louis, MO, USA).¹H NMR and ¹³C NMR spectra were recorded on a NMR spectra were recorded with a Bruker DRX 600 MHz spectrometer (Bruker Daltonics Inc., Billerica, MA, USA). The peaks patterns are indicated as follows: s, singlet; d, doublet; t, triplet; q, quartet; dd, doublet doublet; m, multiplet. The spectra were recorded with TMS as internal standard. Coupling constants (J) were reported in hertz (Hz). Chemical shifts were given in part per million (ppm) on the delta scale. Analytical Thin Layer Chromatography (TLC) was carried out on silica gel F₂₅₄ plates with visualization by ultraviolet radiation. HRMS spectra were recorded on a Bruker MicrOTOF-Q II (Bruker Daltonics Inc., Billerica, MA, USA) mass spectrometer. Inhibition studies were performed on an Agilent-8453 UV-visible spectrometer (Santa Clara, CA, USA).

Examination of the optical and fluorescence images of the leaves was conducted using an Olympus (BX51 W/DP70) microscope with a U-MWG2 filter set (510–550 nm excitation, exposure time of 142 ms). UV-visible absorption spectra were recorded using a single beam Agilent 8453 UV–visible Spectrometer (Agilent Technologies). The Raman spectra of the PDA film at locations on the leaf for active and inactive stomata respectively were collected with a Raman microscope (Horiba Scientific, LabRAM HR Evolution) using a 785 nm excitation laser. The leaf sample was immobilized onto a glass slide using double sided tape. For scanning electron microscopy (SEM), the leaf samples were immobilized onto an SEM plate using carbon tape as well as silver paste, and the leaves were sputter coated with platinum prior to SEM. SEM of the stomata was conducted with a JEOL(JSM-6330F) FE-SEM.