Blood samples were collected into vacuum test tubes after eight hours of fasting. Biochemical data included the fasting blood glucose (FBG), serum uric acid (SUA) (ULN, 420 μmol/L), serum aspartate aminotransferase (AST), alanine aminotransferase (ALT) (ULN, 40 U/L), total plasma cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), and high-density lipoprotein cholesterol (HDL-C). All biochemical indicators were measured on a Roche Modular Analytics System (Roche Diagnostics, Basel, Switzerland) and tested in a clinical laboratory using standard operating procedures.
Modular analytics system
Manufactured by Roche
Sourced in Germany, Australia, Switzerland
The Modular Analytics System is a versatile and efficient laboratory equipment platform designed to streamline analytical processes. The core function of the Modular Analytics System is to automate and integrate various analytical tasks, enabling laboratories to optimize their workflow and enhance productivity.
Automatically generated - may contain errors
Lab products found in correlation
Second generation enzyme immunoassay, by Abbott (4 mentions)
Radioimmunoassay, by Abbott (3 mentions)
Cobas integra 800 system, by Roche (1 mentions)
Hem 7080it, by Omron (1 mentions)
Tina quant crp latex high sensitivity assay, by Roche (1 mentions)
Chol kit, by Roche (1 mentions)
Nycocard reader 2, by Axis-Shield (1 mentions)
Discovery w scanner, by Hologic (1 mentions)
Accq tag ultra chemistry kit, by Waters Corporation (1 mentions)
Cell dyn sapphire, by Abbott (1 mentions)
42 protocols using modular analytics system
1
Fasting Blood Biochemical Indicators
2
Blood Sample Collection and Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Fasting blood samples were collected for the measurement of plasma glucose and lipid profile including TC, TG, LDL-C and HDL-C levels. All blood samples were kept in ice at 0 °C and returned to the laboratory within 4 h after collection either for assay or storage. Blood samples including fasting plasma glucose (FPG) and lipid profile were assayed within 6 h after collection and additional aliquots of serum for other assays were stored at −70 °C. Glucose (hexokinase method), TC (enzymatic method), TG (enzymatic method without glycerol blanking) and HDL-C (direct method using PEG-modified enzymes and dextran sulfate) were measured on a Roche Modular Analytics system (Roche Diagnostics GmbH, Mannheim, Germany) using standard reagent kits supplied by the manufacturer of the analyzer. The precision performance of these assays was within the manufacturer's specifications.
3
Comprehensive Diabetes Assessment Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The diabetes‐related metabolic control and complication assessment included an interview with detailed documentation of medical history and current use of medications, anthropometric measurements and biochemical evaluations, and was carried out by qualified diabetes nurses based on the modified European DiabCare protocol18 . Anthropometric parameters including bodyweight, body height, waist and hip circumferences, systolic blood pressure, and diastolic blood pressure were measured. Blood samples were collected from participants for biochemical analyses after fasting for at least 8 h. Fasting plasma glucose and lipid profile including total cholesterol, triglyceride and high‐density lipoprotein cholesterol were measured by the Roche Modular Analytics system (Roche Diagnostics GmbH, Mannheim, Germany). Low‐density lipoprotein cholesterol was calculated using the Friedewald formula19 . Glycated hemoglobin (HbA1c) was measured by the Cobas Integra 800 System (Roche Diagnostics GmbH). All laboratory assays were carried out within the manufacturer's specifications using standard protocols under the Department of Chemical Pathology at the PWH. The laboratory is accredited by the Australian National Association of Testing Authorities. Good glycemic control was defined as HbA1c <7.0%20 .
4
Lipid Profile and Blood Pressure Study
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Fasting lipid profiles and blood pressure were monitored at baseline and after 14 days of green tea extract and soy isoflavones, respectively. Plasma lipid profile including total cholesterol, triglycerides, and high-density lipoprotein cholesterol (HDL-C) was measured on a Roche Modular Analytics system (Roche Diagnostics GmbH, Mannheim, Germany) using standard reagent kits supplied by the manufacturer of the analyzer and LDL-C level was estimated by using the Friedewald formula (27 (link)) or directly measured when the triglyceride level was over 4.5 mmol/L. After 5 min of resting seated, clinic blood pressure and heart rate was measured four times at 2-min intervals in the dominant arm with an automatic device (Omron HEM 7080IT, Omron Healthcare). The average of the last three measurements was used in the statistical analyses.
5
Serum Biomarker Assessment Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Serum hepatitis B surface antigen (HBsAg) and the HCV antibody were tested by radio-immunoassay (Abbott Laboratories, North Chicago, IL) and second-generation enzyme immunoassay (Abbott). Serum biochemistries were measured using a Roche/Hitachi Modular Analytics System (Roche Diagnostics GmbH, Mannheim, Germany). The serum AFP level was tested using a radio-immunoassay kit (Serono Diagnostic SA, Coinsin/VD, Switzerland).
6
Serum Markers for Viral Hepatitis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Venous blood samples were collected after an overnight fast. Serum HBV surface antigen was tested by radioimmunoassay (Abbott Laboratories, North Chicago, IL, USA), and HCV antibodies were tested by a second-generation enzyme immunoassay. The serum biochemical markers were measured with a Roche/Hitachi Modular Analytics System (Roche Diagnostics GmbH, Mannheim, Germany).
7
Fasting Blood Biomarker Measurements
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All participants underwent a blood draw during the morning (8–10 a.m.) in a state of fasting for at least 8 h and all samples were stored at −80 °C until analysis. Roche Modular Analytics System was used to perform all biochemical analyses including fasting glucose, lipid profile, and transaminases, and a direct method of homogeneous enzymatic assay was used to measure low-density lipoprotein (LDL) cholesterol and high-density lipoprotein (HDL) cholesterol. The Immunolite Diagnostic Products Co, Los Angeles, CA, USA with solid-phase chemiluminescent enzyme immunoassay kit was used to measure fasting insulin levels. As already reported [38 (link),39 (link),40 (link)], the intra-assay coefficient of variation (CV) was <5.5%.
8
Biochemical Profiling of Fasting Samples
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Samples were collected in the morning between 8 and 10 a.m., after an overnight fast of at least 8 h and stored at −80 °C until being processed. All biochemical analyses including fasting plasma glucose, total cholesterol, fasting plasma TG, Alanine Transaminase (ALT), Aspartate Aminotransferase (AST), and γ-Glutamyltransferase (γGT) were performed with a Roche Modular Analytics System in the Central Biochemistry Laboratory of our Institution. Low-Density Lipoprotein (LDL) cholesterol and HDL cholesterol were determined by a direct method (homogeneous enzymatic assay for the direct quantitative determination of LDL and HDL cholesterol). Fasting insulin levels were measured by a solid-phase chemiluminescent enzyme immunoassay using commercially available kits (Immunolite Diagnostic Products Co., Los Angeles, CA, USA). The intra-assay coefficients of variations (CV) was <5.5%, as already widely reported in our previous studies [32 (link),33 (link),34 (link),35 (link),36 (link)].
9
Metabolite Quantification in Cell Samples
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Glucose and lactate concentrations were determined in cell homogenates and media by commercial assays (Modular Analytics System™, Roche Diagnostics, Mannheim, Germany). Three of the 15 dishes per experimental setting were pooled for these analyses.
10
Serum Lipid Profiling Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Blood samples were obtained during the 10- and 15-year follow-up physical examinations. The concentrations (mmol/L) of total cholesterol, LDL, HDL, and triglycerides (TAG) were measured in serum using homogenous enzymatic colorimetric methods on a Modular Analytics System from Roche Diagnostics GmbH Mannheim according to the manufactures instructions. External controls were used in accordance with the guidelines of the German Society of Clinical Chemistry and Laboratory Medicine. The ratio of total to HDL cholesterol (TOTAL:HDL) was calculated by dividing total cholesterol by HDL.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!