Sections were heated for 60 mins at 70 °C prior to staining with haematoxylin and eosin (H&E) using a Leica XL automated stainer with a Leica CV5030 automated coverslipper (Leica Biosystems, Germany). The staining protocol used included 2 Xylene (Acquasience, UK) dewaxing steps (2 mins each), 3 Xylene clearance steps in IMS (2 mins each) followed by staining in Harris Haematoxylin (Leica Biosystems, Germany; 5 min), washing the section, immersion in 1% hydrochloric acid in 50% IMS (10 s), wash 5 min), Eosin (Leica Biosystems, Germany), 3 dehydration steps in IMS (2 mins each), 2 alcohol clearance steps in Xylene (2 mins each) before mounting in Pertex mountant (CellPath Ltd., UK) and covering with a coverslip.
Cv5030 automated coverslipper
Manufactured by Leica
Sourced in Germany
The Leica CV5030 is an automated coverslipper designed for laboratory use. It consistently and precisely applies coverslips to prepared microscope slides.
Automatically generated - may contain errors
2 protocols using cv5030 automated coverslipper
1
Haematoxylin and Eosin Staining Protocol
2
Automated Immunohistochemical Analysis of CC10 and MUC5AC
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Automated immunohistochemical analysis was performed with the Leica BOND-MAX system (Leica Biosystem Nussloch GmbH) by using a Leica BOND Polymer Refine Detection kit for anti-CC10 (Clone E11, Santa Cruz Biotechnologies, Inc., Santa Cruz, CA, USA) and anti-MUC5AC (AM32160PU-N, ACRIS Antibodies GmbH, Herford, Germany) primary antibodies. After staining, the slides were washed, dehydrated, and coverslipped by using the Leica CV5030 automated coverslipper (Leica Biosystems Nussloch GmbH). Digital images were generated by using the NanoZoomer 2.0 slide scanner (Hamamatsu Photonics, Hamamatsu, Japan).
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