Cells pre-treated with 30 nM miRNA inhibitor mix or scramble RNA for 24 h were washed 3 times in PBS. DAPI stain solution (300 nM; Thermo Fisher Scientific, Inc.) was added at a sufficient quantity to cover the cells, then protected from light and incubated at room temperature for 5 min. The cells were washed 3 times in PBS and visualized by fluorescence microscopy. Normal nuclear and apoptotic bodies were visualized and distinguished.
Dapi stain solution
Manufactured by Thermo Fisher Scientific
Sourced in Italy
DAPI stain solution is a fluorescent dye used for nucleic acid staining. It binds strongly to DNA and emits blue fluorescence when excited by ultraviolet light. The solution is commonly used in various biological and research applications, such as cell visualization, cell counting, and fluorescence microscopy.
Automatically generated - may contain errors
3 protocols using dapi stain solution
1
Apoptosis Assay with miRNA Inhibitor
2
Visualizing CLL Cells Using Confocal Microscopy
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Total CLL cell population from patients (106 cells/mL) were incubated with PE-conjugated anti-CD5 antibody (Miltenyi Biotec, Bergisch Gladbach, Germany) and FITC-conjugated p1 peptide (10 μg/mL) and DAPI on ice for 20 min in the dark. After washing with Perm/WashTM Buffer solution (Becton Dickinson Italia S.p.A, Milan, Italy), cells were incubated with DAPI stain solution (Thermo Fisher, Waltham, MA, USA) at room temperature for 5 min in the dark. After extensive washing, cells were mounted under a cover slip, and visualized by confocal microscopy. Pictures were captured with a Leica TCS SP2 confocal microscope with a HCX PL APO 63.0×/1.40 oil UV objective (NA1.40) in glycerol and acquired with Leica Confocal Software Version 2.61 (Wetzlar, Germany). Image manipulation was performed with Adobe Photoshop CC 2015 software (San Jose, CA, USA)
3
Laser-Induced Astrocyte Damage Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Primary astrocytes plated on gridded coverslip in 35 mm imaging dish coated with polylysine were targeted within the nucleus with previously discussed laser settings. Cells were fixed with 4% paraformaldehyde within 30 min of laser damage. Cells were then placed in 3% TritonX in PBS blocking buffer with 5% BSA. Astrocytes were stained with anti-PAR polyclonal antibody (rabbit, 4336-BPC-100, Trevigen) and 4′,6-diamidino-2-phenylindole (DAPI) 300 nM DAPI stain solution (Thermo Fisher). Following staining, targeted cells were imaged for PAR (450 nm excitation, 510 nm emission) and DAPI signal (358 nm excitation, 463 nm emission).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!