Alexa fluor 633 goat anti mouse igg h l a21050

Mouse anti-NP monoclonal antibody (mAb) and rabbit anti-NP polyclonal antibody (pAb) were prepared in our laboratory by using conventional methods. The following primary antibodies were purchased from commercial resources: rabbit anti-V5 pAb (AB3792, Merck Millipore, Darmstadt, Germany); rabbit anti-GAPDH pAb (10494-1-AP), rabbit anti-importin β pAb (10077-1-AP), rabbit anti-LaminB1 pAb (12987-1-AP), rabbit anti-Mx1 pAb (13750-1-AP) and rabbit anti-PLSCR1 pAb (11582-1-AP) from Proteintech (Wuhan, China); mouse anti-actin mAb (sc-47778), mouse anti-p-Ser mAb (sc-81514) and mouse anti-p-Tyr mAb (sc-508) from Santa Cruz (Dallas, TX); mouse anti-Flag mAb (F3165), mouse anti-Myc mAb (M4439), mouse anti-V5 mAb (V8012), rabbit anti-Flag pAb (F7425) and rabbit anti-Myc pAb (C3965) from Sigma-Aldrich. The secondary antibodies used in the western blotting were DyLight 800 goat anti-mouse IgG (H+L) (072-07-18-06) and DyLight 800 goat anti-rabbit IgG (H+L) (072-07-15-06), purchased from KPL (Gaithersburg, MD); the secondary antibodies used in the confocal microscopy were Alexa Fluor 488 donkey anti-rabbit IgG (H+L) (A21206) and Alexa Fluor 633 goat anti-mouse IgG (H+L) (A21050) obtained from Life Technologies.

5′-pppRNA and poly(I:C) were purchased from InvivoGen, and were used at a final concentration of 0.5 µg/mL and 10 µg/mL, respectively. MG132, IFN-β, IFN-β ELISA kit, Glutathione-Sepharose, and the Dual-Luciferase Reporter Assay System were purchased from Sigma Aldrich, R&D Systems, PBL Interferon Source, GE Healthcare, and Promega, respectively. Antibodies against the following IAV proteins were generated in our Laboratory: PB2, PB1, PA, and NP. Anti-TRAF3 (sc-6933), anti-TRIM35 (sc-100880), and anti-ubiquitin (sc-8017) were from Santa Cruz. Anti-TRAF3 (4729), anti-MAVS (24930), anti-K63-linkage specific polyubiquitin (5621), anti-K48-linkage specific polyubiquitin (8081) were from Cell Signaling. Anti-Flag (F1804; F7425), anti-Myc (C3965; M4439), anti-V5 (V8012), and anti-TRIM35 (SAB2103161) were from Sigma Aldrich. Anti-IFITM3 (11714-1-AP), anti-HA (51064-2-AP), and anti-GAPDH (10494-1-AP) were from Proteintech. Anti-V5 (AB3792) was from Millipore. Anti-GFP (ab6556), and anti-GST (ab58626) were from Abcam. Mouse IgG (A7028) was from Beyotime. Alexa Fluor 633 goat anti-mouse IgG (H + L) (A21050) and Alexa Fluor 488 donkey anti-rabbit IgG (H + L) (A21206) from Life Technologies were used for confocal microscopy. DyLight 800 goat anti-mouse IgG (H + L) (072-07-18-06) and DyLight 800 goat anti-rabbit IgG (H + L) (072-07-15-06) were from KPL.