Dimension fastscan bio

Manufactured by Bruker

Sourced in Germany, United States

The Dimension FastScan Bio is an atomic force microscope (AFM) designed for high-speed, high-resolution imaging of biological samples. It features advanced scanning capabilities to capture real-time dynamics of live cells and biomolecules.

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Dimension FastScan Bio AFM (11 citations) Dimension FastScan Bio microscope (2 citations) Dimension FastScan (109 citations) FastScan Bio (19 citations) FastScan (15 citations)

16 protocols using dimension fastscan bio

Atomic Force Microscopy of Topographical Imaging

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Topographical
images have been recorded
by AFM in the tapping mode with a Bruker Dimension FastScan Bio instrument.
For measurement in air, the images were scanned using silicon cantilevers
(Olympus) with a resonance frequency of 300 kHz and a force constant
of 26 N m^–1. The scanned image size was 1 ×
1 μm². For the measurements in liquid environment,
FastScan-D and SNL cantilevers having resonance frequencies of 110
and 65 kHz, and force constants of 0.25 and 0.35 N m^–1, respectively, were used.

Griffo A., Hähl H., Grandthyll S., Müller F., Paananen A., Ilmén M., Szilvay G.R., Landowski C.P., Penttilä M., Jacobs K, & Laaksonen P. (2017). Single-Molecule Force Spectroscopy Study on Modular Resilin Fusion Protein. ACS Omega, 2(10), 6906-6915.

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Comprehensive Characterization of Colloidal Nanomaterials

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Zeta potential
measurements were
collected using a Zeta potential and nanoparticle analyzer (Delsa
Nano C, Beckman Coulter, USA). Transmission electron microscopy (TEM)
images were obtained on a Talos F200X G2. Atomic force microscopy
images were determined using a Dimension FastScan Bio, Bruker. Fourier
transform infrared spectroscopy (FTIR) spectra were recorded on a
Tensor 27 FTIR spectrometer (Nicolet 6700). Elemental analysis was
carried out using X-ray photoelectron spectroscopy on a Kratos Axis
Ultra DLD X-ray photoelectron spectrometer with 60 W monochromated
Mg Kα radiation as the X-ray source for excitation. The C 1s
peak (284.6 eV) was used for internal calibration. Electron paramagnetic
resonance (EPR) signals of the CD colloidal solution were recorded
on a Bruker EMX plus 9.5/12 spectrometer at room temperature. Titration
experiments were carried out with a SevenExcellence (Mettler Toledo)
pH electrode. Fe K-edge X-ray absorption fine structure (XAFS) measurements
were performed in fluorescence mode at the BL01B1 beamline of SPring-8
(JASRI), Hyogo, Japan (Prop. nos. 2021A1089 and 2021A1095). The synchrotron
radiation experiments for XAFS measurements were performed at the
BL01B1 beamline in SPring-8 with approval from JASRI (nos. 2021A1089
and 2021A1095).

Zhang T., Pan Z., Wang J., Qian X., Yamashita H., Bian Z, & Zhao Y. (2023). Homogeneous Carbon Dot-Anchored Fe(III) Catalysts with Self-Regulated Proton Transfer for Recyclable Fenton Chemistry. JACS Au, 3(2), 516-525.

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Comprehensive Nanomaterial Characterization

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The nanomorphologies were visualized by field-emission scanning electron microscopy (FESEM, S-4800). Elemental mapping was achieved by transmission electron microscopy (TEM, Tecnai G2 Spirit). A Micromeritics ASAP 2020 surface area and porosity analyzer was used to analyze the pore structure of the separators, and the PSD was obtained by using a DFT model. FTIR spectra were recorded at room temperature on a Bruker Equinox 55 FTIR spectroscope. Contact angle measurements were conducted by using a KRUSS DSA100 machine. The Young’s moduli of the separators were measured by AFM (Bruker Dimension Fastscan Bio) in peak force quantitative nanomechanics mode and analyzed by the Derjaguin–Muller–Toporov model.

Li C., Liu S., Shi C., Liang G., Lu Z., Fu R, & Wu D. (2019). Two-dimensional molecular brush-functionalized porous bilayer composite separators toward ultrastable high-current density lithium metal anodes. Nature Communications, 10, 1363.

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Comprehensive Characterization of Ti Coating

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The characterizations of the samples were performed at different steps of fabricating the coating. Scanning electron microscopy (SEM; Merlin, Zeiss, Germany) was employed to observe the surface morphology of Ti coating in each step of construction and the thickness of the final coating. X-ray photoelectron spectroscopy (XPS; Escalab 250, Thermo-VG Scientific, USA) was then performed to analyze the elemental components and valence states on the Ti surface. Water contact angle measurement (OCA40 Micro, Dataphysics, Germany) and confocal laser scanning microscope (CLSM; LSM700, Zeiss, Germany) were employed to evaluate the hydrophilicity and surface roundness of different Ti substrates. Young's modulus of Ti-CCH was measured by atomic force microscopy (Dimension fastscan bio, Bruker, Germany) in the peak force quantitative nanomechanics mode and was analyzed by the Derjaguin–Muller–Toporov model. To further evaluate the mechanical stability of QCMC/COL/HAP coating, Scratch test and SEM were employed to evaluate the mechanical stability of QCMC/COL/HAP coating on Ti-CCH.

Lin R., Wang Z., Li Z, & Gu L. (2022). A two-phase and long-lasting multi-antibacterial coating enables titanium biomaterials to prevent implants-related infections. Materials Today Bio, 15, 100330.

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AFM Imaging of MRNIP-GFP Droplets

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AFM images were captured in tapping mode using icon scanner AFM instrument (Dimension FastScan Bio, Bruker, Germany) equipped with a high-resonance microscope. Parameters of the cantilever we used were as follows: length, 70 μm; width, 10 μm; thickness, 0.6 μm; frequency, 150 kHz; spring constant, 0.35-1.4 N/m (Scanasyst-Fluid + , Bruker). Microscope was used to observe and select MRNIP-GFP droplets for nanoscale imaging with AFM. AFM imaging conditions were as follows: scan size, 5.00 × 5.00 μm²; scan rate, 0.501 Hz; pixel size, 20 × 20 pixels. All imaging was performed at room temperature. NanoScope Analysis software (Version 1.40, Bruker Corporation) was used to process the images. Surface tension was calculated using the following formula:^{54 (link)} (3)

σ = \frac{F_{ret}}{2 π r} = \frac{F_{pull - in}}{2 π r \cos (θ_{e})}

, where F_ret is the retention force, F_pull-in is the pull-in force, r is the radius of the nanoneedle, and θ_e is the equilibrium contact angle between the meniscus and nanoneedle.

Wang Y.L., Zhao W.W., Bai S.M., Feng L.L., Bie S.Y., Gong L., Wang F., Wei M.B., Feng W.X., Pang X.L., Qin C.L., Yin X.K., Wang Y.N., Zhou W., Wahl D.R., Liu Q., Chen M., Hung M.C, & Wan X.B. (2022). MRNIP condensates promote DNA double-strand break sensing and end resection. Nature Communications, 13, 2638.

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Measuring Young's Modulus of Fresh Tissues

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The measurements of Young's modulus of fresh tissues were carried out using Dimension FastScan Bio (Bruker, MA, USA) by the Instrumental Analysis Center of Shanghai Jiao Tong University following a standardized procedure. Data were analyzed by NanoScope Analysis v.180r1 (Veeco Instruments Inc., NY, USA) according to the manufacturer's instructions.

Wen D., Gao Y., Liu Y., Ho C., Sun J., Huang L., Liu Y., Li Q, & Zhang Y. (2023). Matrix stiffness‐induced α‐tubulin acetylation is required for skin fibrosis formation through activation of Yes‐associated protein. MedComm, 4(4), e319.

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Atomic Force Microscopy of Alpha-Synuclein Fibrils

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Mica was freshly cleaved before being treated to create a positive surface charge by adding poly-l-lysine (70–150 kDa) at 15 µg/mL for 10 s followed by drying with nitrogen. A sample volume of 90 μL of protein (WT αSyn, L38M, Y39A or S42A) was taken at the end point of a fibril growth assay (as described above) before being deposited at a concentration of 30 μM onto poly-l-lysine treated mica and allowed to incubate for 4 min. The mica surface was then rinsed with buffer (50 mM sodium phosphate buffer, 300 mM KCl, pH 7.5) via fluid exchange, maintaining the samples in a liquid environment. AFM observations were performed in liquid in tapping mode using a Dimension FastScan Bio with FastScan-D-SS probes (Bruker) in the same buffer. The force applied by the tip on the sample was minimized by maximizing the set point whilst maintaining tracking of the surface. Heights of single particles were measured automatically using routines written in MATLAB (https://github.com/George-R-Heath/Particle-Detect). Heights and lengths of fibrils were measured either automatically using MATLAB (https://github.com/George-R-Heath/Correlate-Filaments) or manually in ImageJ for densely packed overlapping fibrils.

Ulamec S.M., Maya-Martinez R., Byrd E.J., Dewison K.M., Xu Y., Willis L.F., Sobott F., Heath G.R., van Oosten Hawle P., Buchman V.L., Radford S.E, & Brockwell D.J. (2022). Single residue modulators of amyloid formation in the N-terminal P1-region of α-synuclein. Nature Communications, 13, 4986.

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Freeze-fractured LCE Characterization by AFM

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yz cross-sectional samples (coordinate system shown in Fig. 1b) were prepared by encasing samples of LCE within a two-part epoxy glue and then freeze-fracturing by snapping off the exposed LCE. AFM images were acquired using a Bruker Dimension FastScan-Bio, using Bruker FastScan A probes, in air tapping mode at a frequency of 1.4 MHz. Images were acquired at a line rate of approximately 4 Hz at 1024 pixel resolution, then processed with a simple low order line flattening in Bruker Nanoscope Analysis v1.9.

Mistry D., Connell S.D., Mickthwaite S.L., Morgan P.B., Clamp J.H, & Gleeson H.F. (2018). Coincident molecular auxeticity and negative order parameter in a liquid crystal elastomer. Nature Communications, 9, 5095.

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Atomic Force Microscopy Imaging of DNA

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For AFM imaging, 10 μL of purified DNA sample diluted to a final concentration of 0.5 nM in 1× TE (pH 8) with 12.5 mM Mg(Ac)₂ was deposited on a freshly cleaved mica substrate (Agar Scientific, Stansted, UK) and incubated at room temperature for 15 min. An additional 150–180 μL of 1× TE (pH 8) with 12.5 mM Mg(Ac)₂ buffer was added to the sample to facilitate the imaging. The samples were imaged using a Dimension Fastscan Bio (Bruker, Coventry, UK) in tapping mode in liquid with Fastscan D Si₃N₄ cantilevers with a Si tip (Bruker). We used the following imaging parameters: scan rate = 2–8 Hz, 256 samples/line, amplitude setpoint = 150–300 mV, drive amplitude = 3000 mV, integral gain = 1, proportional gain = 5. The data were processed using Nanoscope analysis 1.9.

Confederat S., Sandei I., Mohanan G., Wälti C, & Actis P. (2022). Nanopore fingerprinting of supramolecular DNA nanostructures. Biophysical Journal, 121(24), 4882-4891.

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Visualizing DNA fragments on mica

Cited 2 times

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Visualization of DNA fragments was performed in air with mica as the substrate. Initial samples were diluted 20 × in HEPES buffer with Mg²⁺ ions (40 mM HEPES, 10 mM MgCl₂, pH 7.0). DNA fragments (20 µL) were adsorbed on a freshly cleaved mica surface (SPI Supplies, West Chester, PA, USA) at room temperature for 30 min. Afterwards, the surface was rinsed with distilled water and dried with compressed air to prevent undesired drying of the buffer salts. The AFM scanning was performed using Dimension FastScan Bio (Bruker, USA) in PeakForce Tapping mode. A ScanAsyst-Air probe (Bruker, USA) with a spring constant k of 0.4 N/m was used. The AFM images were processed using the Gwyddion software package. The AFM images were processed in FiberApp to measure lengths of the DNA strands.
In vitro proliferation in bone marrow-derived macrophages
In vitro proliferation of the strains with modified HU protein (R58Q, R61Q, and S74A) was performed as we have described previously [20 (link),38 (link)].

Pavlik P, & Spidlova P. (2022). Arginine 58 is indispensable for proper function of the Francisella tularensis subsp. holarctica FSC200 HU protein, and its substitution alters virulence and mediates immunity against wild-type strain. Virulence, 13(1), 1790-1809.

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