ChIP was performed as described earlier2 (link), 73 (link) with the modification that cells were fixed first with 5mM DTBP (Pierce-Thermo scientific) and then with 1% formaldehyde. Immuno precipitation was performed using anti-GFP antibody (Abcam ab290) or mock IgG (Santacruz). Promoter segment enrichment was analyzed by qPCR using primers P49-56 (Suppl. Table 4 ).
Mock igg
Manufactured by Santa Cruz Biotechnology
Mock IgG is a laboratory reagent used as a control in various immunoassays and experiments. It serves as a non-specific antibody that can be used to establish baseline or background signals in these applications.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using mock igg
1
ChIP Protocol with DTBP and Formaldehyde
2
ChIP Protocol with DTBP and Formaldehyde
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!