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Anti bcl6

Manufactured by Santa Cruz Biotechnology
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Anti-Bcl6 is a laboratory reagent that specifically binds to the Bcl6 protein. Bcl6 is a transcriptional repressor involved in the regulation of gene expression. Anti-Bcl6 can be used to detect and study the Bcl6 protein in various experimental systems.

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Lab products found in correlation

Ra3 6b2, by BD (4 mentions) Anti b220, by BD (3 mentions) Anti cd138, by BD (3 mentions) Anti hdac1, by Cell Signaling Technology (2 mentions) Anti egfp, by Cell Signaling Technology (2 mentions) Anti v5 tag, by Thermo Fisher Scientific (2 mentions) Anti streptavidin, by Merck Group (2 mentions) Anti rabbit 680lt, by LI COR (2 mentions) Anti mouse alexa fluor 633, by Thermo Fisher Scientific (2 mentions) Alexa anti mouse 488, by BioLegend (2 mentions) Anti beta tubulin, by Cell Signaling Technology (2 mentions) Anti mouse 800cw, by LI COR (2 mentions) Anti histone h3, by Cell Signaling Technology (2 mentions) Anti hsp90, by Cell Signaling Technology (2 mentions) Anti bcl 2, by BD (2 mentions) Anti ki67, by Thermo Fisher Scientific (2 mentions) Anti irf4, by Santa Cruz Biotechnology (2 mentions) Hematoxylin eosin, by Thermo Fisher Scientific (2 mentions) Anti gapdh, by Thermo Fisher Scientific (1 mentions) Phosphatase inhibitor cocktail, by Thermo Fisher Scientific (1 mentions)

10 protocols using anti bcl6

1

Comprehensive Antibody Panel for Protein Analysis

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The following antibodies were used in this study: anti-BCL6 (Santa Cruz Biotechnology, sc-7388), anti-beta-tubulin (Cell Signaling, 2146S), anti-Hsp90 (Cell Signaling, 4874S), anti-HDAC1 (Cell Signaling, 2062S), anti-Histone H3 (Cell Signaling, 12648S), anti-eGFP (Cell Signaling, 2956), anti-V5-tag (ThermoFisher Scientific, MA5–15253), anti-Streptavidin (Sigma, 71591–3), anti-Mouse 800CW (LI-COR Biosciences, 926–32211), anti-Rabbit 680LT (LI-COR Biosciences, 925–68021), anti-mouse Alexa Fluor 633 (ThermoFisher Scientific, A-21052), and Alexa anti-mouse 488 (Biolegend, 405319).
Słabicki M., Yoon H., Koeppel J., Nitsch L., Roy Burman S.S., Di Genua C., Donovan K.A., Sperling A.S., Hunkeler M., Tsai J.M., Sharma R., Guirguis A., Zou C., Chudasama P., Gasser J.A., Miller P.G., Scholl C., Fröhling S., Nowak R.P., Fischer E.S, & Ebert B.L. (2020). Small molecule-induced polymerization triggers degradation of BCL6. Nature, 588(7836), 164-168.
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2

Histological and Immunohistochemical Analysis of Mouse Organs

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Histological analysis of mouse organs was performed on 4 μm thick FFPE tissue sections, stained with Hematoxylin & Eosin (H&E) (Thermo Scientific)53 (link). The following primary antibodies and dilutions were used for immunohistochemical analysis: rabbit polyclonal anti-Bcl6 (1:300) (N3, Santa Cruz Biotechnology) and anti-Pax5 (1:400) (Neomarker); rabbit monoclonal anti-CD3 (1:800) (clone SP7, Neomarker) and anti-Ki67 (1:200) (clone SP6, Thermo Scientific); rat monoclonal anti-B220 (1:400) (clone RA3-6B2, BD Biosciences) and anti-CD138 (1:200) (clone 281-2, BD Biosciences); mouse monoclonal anti-BCL2 (1:200) (clone Bcl-2/100, BD Biosciences).
Zhang J., Dominguez-Sola D., Hussein S., Lee J.E., Holmes A.B., Bansal M., Vlasevska S., Mo T., Tang H., Basso K., Ge K., Dalla-Favera R, & Pasqualucci L. (2015). Disruption of KMT2D perturbs germinal center B cell development and promotes lymphomagenesis. Nature medicine, 21(10), 1190-1198.
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3

Comprehensive Immunoblotting Profiling of Cellular Signaling

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Five-7 x 106 cells were prepared by lysis in a buffer made up of 20 mM Tris, 150 mM NaCl, 2 mM EDTA (ethylenediaminetetraacetic acid), 2 mM EGTA (ethylene glycol-tetra-acetic acid), 0.5% v/v Triton X-100 supplemented with protease inhibitor cocktail (Sigma), 1 mM DTT (dithiothreitol; Amersham Biosciences), 1 mM PMSF (phenyl-methyl-sulfonyl fluoride; Sigma), 1 mM okadaic acid (Sigma) and phosphatase inhibitor cocktail (Thermo Scientific). Proteins were subjected to SDS-PAGE, transferred to PVDF membranes and immunoblotted with the following primary Abs: anti-CK2α (provided by Dr. M. Ruzzene, University of Padova, Italy), anti-CK2β, anti-RELA, anti-FOXO1 (Abcam), anti-pRELA S529 (recognizes S527 in mouse), anti-IRF4, anti-BLIMP-1, anti-BCL6 (Santa Cruz), anti-GAPDH (Ambion), anti-pAKT S129 (provided by Dr. M. Ruzzene, University of Padova, Italy), anti-AID (Invitrogen), anti-NOTCH2 (D76A6), anti-pAKT S473, anti-AKT, anti-pERK1/2 T202/Y204, anti-pBTK Y223, anti-ERK1/2, anti-pPTEN S380/T382/T383, PTEN, anti-pFOXO1 S256, anti-pSTAT6 Y705, anti-STAT6 (Cell Signaling). Secondary Abs: anti-rabbit IgG HRP-linked Ab (Cell Signaling), HRP labeled goat anti-mouse IgG (KPL), goat anti-rat IgG HRP-conjugated (Calbiochem), donkey anti-goat IgG HRP-conjugated (Santa Cruz).
Quotti Tubi L., Mandato E., Canovas Nunes S., Arjomand A., Zaffino F., Manni S., Casellato A., Macaccaro P., Vitulo N., Zumerle S., Filhol O., Boldyreff B., Siebel C.W., Viola A., Valle G., Mainoldi F., Casola S., Cancila V., Gulino A., Tripodo C., Pizzi M., Dei Tos A.P., Trentin L., Semenzato G, & Piazza F. (2023). CK2β-regulated signaling controls B cell differentiation and function. Frontiers in Immunology, 13, 959138.
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4

Histological and Immunohistochemical Analysis of Mouse Organs

Cited 1 time
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Histological analysis of mouse organs was performed on 4 μm thick FFPE tissue sections, stained with Hematoxylin & Eosin (H&E) (Thermo Scientific)53 (link). The following primary antibodies and dilutions were used for immunohistochemical analysis: rabbit polyclonal anti-Bcl6 (1:300) (N3, Santa Cruz Biotechnology) and anti-Pax5 (1:400) (Neomarker); rabbit monoclonal anti-CD3 (1:800) (clone SP7, Neomarker) and anti-Ki67 (1:200) (clone SP6, Thermo Scientific); rat monoclonal anti-B220 (1:400) (clone RA3-6B2, BD Biosciences) and anti-CD138 (1:200) (clone 281-2, BD Biosciences); mouse monoclonal anti-BCL2 (1:200) (clone Bcl-2/100, BD Biosciences).
Zhang J., Dominguez-Sola D., Hussein S., Lee J.E., Holmes A.B., Bansal M., Vlasevska S., Mo T., Tang H., Basso K., Ge K., Dalla-Favera R, & Pasqualucci L. (2015). Disruption of KMT2D perturbs germinal center B cell development and promotes lymphomagenesis. Nature medicine, 21(10), 1190-1198.
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5

Histological and Immunohistochemical Analysis of Mouse Organs

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Histological analysis of mouse organs was performed on 4µm-thick
FFPE tissue sections, stained with Hematoxylin & Eosin (Thermo
Scientific) following standard procedures. The following primary antibodies were
used for immunohistochemical analysis: anti-Bcl6 (1:300) (N3, rabbit polyclonal,
Santa Cruz Biotechnology), anti-PAX5 (1:400) (rabbit polyclonal, Neomarker),
anti-CD3 (1:800) (rabbit monoclonal, clone SP7, Neomarker), anti-B220 (1:400)
(rat monoclonal, clone RA3-6B2, BD Biosciences), anti-IRF4 (1:200) (M-17, goat
polyclonal, Santa Cruz Biotechnology), anti-BCL2 human (1:100) (rabbit
polyclonal, Santa Cruz Biotechnology), and anti-CD138 (1:200) (rat monoclonal,
clone 281-2, BD Biosciences).
Zhang J., Vlasevska S., Wells V.A., Nataraj S., Holmes A.B., Duval R., Meyer S.N., Mo T., Basso K., Brindle P.K., Hussein S., Dalla-Favera R, & Pasqualucci L. (2017). The Crebbp acetyltransferase is a haploinsufficient tumor suppressor in B cell lymphoma. Cancer discovery, 7(3), 322-337.
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6

Histological Analysis of Mouse Lymphoid Organs

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Histological analysis of mouse lymphoid organs was performed on
3-μm-thick FFPE tissue sections, stained with Hematoxylin & Eosin
(Thermo Scientific) following standard procedures. The following primary
antibodies were used for immunohistochemical analysis: anti-Bcl6 (1:300)
(N3, rabbit polyclonal, Santa Cruz Biotechnology); biotin-conjugated
anti-PNA (1:200) (Vector Laboratories); biotin-conjugated anti-B220 (1:400)
(RA3–6B2, rat monoclonal, Pharmingen 553086) and anti-CD3 (1:800)
(SP7, rabbit monoclonal, NeoMarkers RM9107). GC numbers, size, and overall
area were calculated using the ImageJ software on scanned images obtained
with a Leica SCN400 slide scanner (Schindelin et al., 2015 (link)).
Meyer S.N., Scuoppo C., Vlasevska S., Bal E., Holmes A.B., Holloman M., Garcia-Ibanez L., Nataraj S., Duval R., Vantrimpont T., Basso K., Brooks N., Dalla-Favera R, & Pasqualucci L. (2019). Unique and Shared Epigenetic Programs of the CREBBP and EP300 Acetyltransferases in Germinal Center B Cells Reveal Targetable Dependencies in Lymphoma. Immunity, 51(3), 535-547.e9.
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7

Immunohistochemical Identification of Tfh Cells

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Immunohistochemistry was performed on formalin-fixed and paraffin-embedded 4-μM tissue sections. Sections were incubated with optimal concentrations of primary monoclonal antibodies, including anti-CD38, anti-CD20, anti-CD4, anti-PD-1 (R&D, AF1086, Abington, UK) and anti-Bcl-6 (Santa Cruz biotechnology, sc-858, Santa Cruz, CA, USA) for 1 hr at RT and then incubated with biotinylated goat anti-rat, goat-rabbit and biotinylated rabbit anti-mouse antibody (Zhongshan Goldenbridge Biotech, Beijing, China), respectively. The avidin-biotin-peroxidase system with 3-amino-9-ethyl-carbazole (AEC) (red color) or Vector blue (blue color) as substrates was used to perform double staining. Based on the double immunostaining, PD-1 and Bcl-6 double positive cells in liver tissue, and CXCR5 and CD4 double positive cells in the spleen were identified as Tfh cells. The absolute number of Tfh cells was independently counted by two pathologists from five representative high-power microscopic fields (400 magnification) relative to the area most involved by the inflammatory infiltrate (28 (link)).
Wang L., Sun Y., Zhang Z., Jia Y., Zou Z., Ding J., Li Y., Xu X., Jin L., Yang T., Li Z., Sun Y., Zhang J.Y., Lv S., Chen L., Li B., Gershwin M.E, & Wang F.S. (2015). CXCR5+ CD4+ T Follicular Helper Cells Participate in the Pathogenesis of Primary Biliary Cirrhosis. Hepatology (Baltimore, Md.), 61(2), 627-638.
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8

Comprehensive Antibody Panel for Protein Analysis

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The following antibodies were used in this study: anti-BCL6 (Santa Cruz Biotechnology, sc-7388), anti-beta-tubulin (Cell Signaling, 2146S), anti-Hsp90 (Cell Signaling, 4874S), anti-HDAC1 (Cell Signaling, 2062S), anti-Histone H3 (Cell Signaling, 12648S), anti-eGFP (Cell Signaling, 2956), anti-V5-tag (ThermoFisher Scientific, MA5–15253), anti-Streptavidin (Sigma, 71591–3), anti-Mouse 800CW (LI-COR Biosciences, 926–32211), anti-Rabbit 680LT (LI-COR Biosciences, 925–68021), anti-mouse Alexa Fluor 633 (ThermoFisher Scientific, A-21052), and Alexa anti-mouse 488 (Biolegend, 405319).
Słabicki M., Yoon H., Koeppel J., Nitsch L., Roy Burman S.S., Di Genua C., Donovan K.A., Sperling A.S., Hunkeler M., Tsai J.M., Sharma R., Guirguis A., Zou C., Chudasama P., Gasser J.A., Miller P.G., Scholl C., Fröhling S., Nowak R.P., Fischer E.S, & Ebert B.L. (2020). Small molecule-induced polymerization triggers degradation of BCL6. Nature, 588(7836), 164-168.
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9

Cul3 Overexpression in Confocal Microscopy

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Human pcDNA3-myc-Cul3 and pcDNA3-myc-Cul3mut (L52AE55A) were previously described (Mathew et al., 2012 (link)). pcDNA3-Bcl6 was provided by B. Baron (University of Chicago, Chicago, IL). Anti-Myc (71D10 from Cell Signaling Technology or 9E10 from EMD Millipore) and anti-Bcl6 (Santa Cruz Biotechnology, Inc.) were used to stain confocal microscopy slides as described previously (Mathew et al., 2012 (link)).
Mathew R., Mao A.P., Chiang A.H., Bertozzi-Villa C., Bunker J.J., Scanlon S.T., McDonald B.D., Constantinides M.G., Hollister K., Singer J.D., Dent A.L., Dinner A.R, & Bendelac A. (2014). A negative feedback loop mediated by the Bcl6–cullin 3 complex limits Tfh cell differentiation. The Journal of Experimental Medicine, 211(6), 1137-1151.
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10

Histopathological Evaluation of Lymphoid Tissues

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5μm thick sections of formalin-fixed, paraffin embedded mouse tissues were stained with H&E. Based on lymphoid follicle morphology, a pathological score was attributed to the spleen and lymph nodes of each 10-12-month-old mouse analyzed (normal=0; reactive=1; reactive>atypical=2; atypical>reactive=3; atypical=4; atypical>lymphomatous=5; lymphomatous>atypical=6; lymphomatous=7). Slides were analyzed in blind by a certified hematopathologist (MP) and two investigators. The following antibodies were used: anti-B220 (RA3-6B2, Serotec), anti-Ki67 (SP6, Neo Markers), anti-CD3 (1F4, Biorad), anti-BCL6 (Rabbit polyclonal, Santa Cruz), anti-BCL2 (C21, Santa Cruz), anti-Multiple Myeloma 1/Interferon Regulatory Factor 4 protein (MUM1/IRF4) (3E4, Biolegend) (31 (link)).
Riva F., Ponzoni M., Supino D., Bertilaccio M.T., Polentarutti N., Massara M., Pasqualini F., Carriero R., Innocenzi A., Anselmo A., Veliz-Rodriguez T., Simonetti G., Anders H.J., Caligaris-Cappio F., Mantovani A., Muzio M, & Garlanda C. (2019). IL-1R8 Deficiency Drives Autoimmunity-Associated Lymphoma Development. Cancer immunology research, 7(6), 874-885.
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