β nicotinamide adenine dinucleotide 2 phosphate reduced tetrasodium salt nadph

Manufactured by Merck Group

Sourced in United States

β-Nicotinamide adenine dinucleotide 2′-phosphate reduced tetrasodium salt (NADPH) is a coenzyme used in various biochemical reactions. It is the reduced form of NADP+ and serves as an electron donor in anabolic reactions.

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Lab products found in correlation

Reduced glutathione, by Merck Group (1 mentions) Phenacetin, by Merck Group (1 mentions) Tolbutamide, by Merck Group (1 mentions) Celecoxib, by Merck Group (1 mentions) Nicardipine hydrochloride, by Tokyo Chemical Industry (1 mentions) Milli q water purification system, by Merck Group (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Dulbecco s modified eagle medium dmem, by Thermo Fisher Scientific (1 mentions) Methanol, by Tedia (1 mentions) Rat liver microsomes, by Xenotech (1 mentions) Hplc grade acetonitrile, by Tedia (1 mentions)

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4 protocols using β nicotinamide adenine dinucleotide 2 phosphate reduced tetrasodium salt nadph

Synthesis and Enzymatic Characterization of MRS5980

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MRS5980 was synthesized as described [8 (link)]. β-Nicotinamide adenine dinucleotide 2′-phosphate reduced tetrasodium salt (NADPH) and reduced glutathione were purchased from Sigma-Aldrich (St. Louis, MO). All other reagents were of the highest grade commercially available. Livers from untreated 6- to 8-week-old male C57BL/6J mice were used to prepare mouse liver microsomes (MLMs) as previously described [4 (link)]. Human liver microsomes (HLMs), recombinant cytochrome P450 (CYP) 1A1, 1A2, 1B1, 2A6, 2B6, 2C9, 2C9*2, 2C9*3, 2C19, 2D6, 2E1, 3A4, and 3A5 were purchased from BD Gentest Corp. (Woburn, MA, USA).

Fang Z.Z., Tosh D.K., Tanaka N., Wang H., Krausz K.W., O'Connor R., Jacobson K.A, & Gonzalez F.J. (2015). Metabolic mapping of A3 adenosine receptor agonist MRS5980. Biochemical pharmacology, 97(2), 215-223.

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In Vitro Assessment of Cytochrome P450 Interactions

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Nicardipine hydrochloride (N0635, purity: >98.0%) and saxagliptin hydrate (A3435, purity: >98.0%) were purchased from Tokyo Chemical Industry (Tokyo, Japan). Midazolam, 1′-hydroxymidazolam (purity: >97.0%), and ketoconazole (purity: 99.0–101.0%) were kindly provided by Gachon University. Phenacetin (77440, purity: >98.0%), tolbutamide (T0891, purity: 97.0–103.0%), celecoxib (SML3031, purity: >98.0%),and β-Nicotinamide adenine dinucleotide 2′- phosphate reduced tetrasodium salt (NADPH) (N1630, purity: >93.0%) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Human liver microsome (HLM), rat liver microsome (RLM), and recombinant rat CYP3A2 (rCYP3A2) were obtained from Xenotech (Lenexa, KS, USA). All other chemicals and solvents were of reagent or HPLC grade.

Lee J.M., Yoon J.H., Maeng H.J, & Kim Y.C. (2024). Physiologically Based Pharmacokinetic (PBPK) Modeling to Predict CYP3A-Mediated Drug Interaction between Saxagliptin and Nicardipine: Bridging Rat-to-Human Extrapolation. Pharmaceutics, 16(2), 280.

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Stereoselective Synthesis of Praziquantel

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(R,S)-PZQ, (R)-PZQ, (S)-PZQ and 4-hydroxyl-PZQ were synthesized by Prof. Dequn Sun et al (Marine College, Shandong University at Weihai). The enantiomeric excess values of (R)-PZQ and (S)-PZQ were >99% by HPLC. β-Nicotinamide adenine dinucleotide 2′-phosphate reduced tetrasodium salt (NADPH) was purchased from Sigma-Aldrich (St. Louis, MO, USA). All other reagents were of the highest grade commercially available.

Wang H., Fang Z.Z., Zheng Y., Zhou K., Hu C., Krausz K.W., Sun D., Idle J.R, & Gonzalez F.J. (2014). Metabolic profiling of praziquantel enantiomers. Biochemical pharmacology, 90(2), 166-178.

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Quantitative Analysis of PCB and PCBG

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Reference standards of PCB and PCBG (purity both ≥98% by HPLC at 280 nm, identified by ¹H-NMR, ¹³C-NMR) were prepared in the Laboratory of Chemistry of Chinese Medicinal Materials of Capital Medical University (Beijing, China). Genistein (Internal standard, IS) was provided by Shenyang Pharmaceutical University (Shenyang, China). HPLC grade acetonitrile and methanol were obtained from TEDIA Company (Fairfield, OH, USA). Cryopreserved hepatocytes from Spague-Dawley (SD) rats and rat liver microsomes were purchased from XenoTech LLC (Lenexa, KS, USA). β-Nicotinamide adenine dinucleotide 2′-phosphate reduced tetrasodium salt (NADPH) and dimethyl sulfoxide (DMSO, BioReagent, ≥99.7%) were purchased from Sigma-Aldrich Co. (St. Louis, MO, USA). Dulbecco’s Modified Eagle Medium (DMEM) and fetal bovine serum (FBS) were purchased from Invitrogen (Grand Island, NY, USA). All other chemicals were of analytical grade. Distilled water was prepared by a Milli-Q water purification system from Millipore (Molsheim, France).

Guo W.W., Qiu F., Chen X.Q., Ba Y.Y., Wang X, & Wu X. (2016). In-vivo absorption of pinocembrin-7-O-β-D-glucoside in rats and its in-vitro biotransformation. Scientific Reports, 6, 29340.

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