Cytosine β d arabinofuranoside hydrochloride c6645

Native BoNT/C and BoNT/A1 were purified as previously described [66 (link), 67 (link)]. Cytosine β-D-arabinofuranoside hydrochloride (C6645), DNAse I from bovine pancreas (DN25), poly-L-lysine hydrobromide (P1274) and trypsin (T4799) were from Sigma Aldrich. μ-Conotoxin GIIIB is from Alomone, Jerusalem, Israel. Primary antibodies: anti-SNAP-25 (SMI81, ab24737) was from Abcam. Anti-SNAP-25 (cleaved) and syntaxin-1A/1B polyclonal antibodies were produced in our laboratory and previously characterized [37 (link), 68 (link)]. Secondary antibodies conjugated to HRP were from Calbiochem; secondary antibodies for immunofluorescence conjugated to Alexa Fluorophores 488 or 555 and α-Bungarotoxin conjugated to Alexa 647 were from Thermo Scientific, Waltham, MA, USA.

Cytosine β-D-arabinofuranoside hydrochloride (C6645), DNase I from bovine pancreas (DN25), poly-L-lysine hydrobromide (P1274) and trypsin (T4799) were purchased from Sigma Aldrich (St. Louis, Missouri, USA).
Primary antibodies: anti-SNAP-25 (SMI81, ab24737) was purchased from Abcam(Cambridge, UK), while anti-syntaxin-1A (STX-1A, 110111) and anti-VAMP-2 were purchased from Synaptic System (Göttingen, Germany). Secondary antibodies: HRP-conjugated Ab were purchased from Calbiochem^® (San Diego, CA, USA), Alexa Fluorophores 488- or 555-conjugated Ab were from Thermo-Fisher Scientific (Waltham, MA, USA). Native TeNT was purified as previously described [58 (link)].
Inhibitors: myricetin [3,3′,4′,5,5′7-Hexahydroxyflavone] and Curcumin [(E,E)-1,7-bis(4-Hydroxy-3- methoxyphenyl)-1,6-heptadiene-3,5-dione] were purchased from Sigma Aldrich. PX12 [2-[(1-Methylpropyl) dithio]-1H-imidazole] and Ebselen [2-Phenyl-1,2-benzisoselenazol3(2H)-one] were purchased from Santa Cruz Biotechnology (Dallas, TX, USA). 4-Bromobenzaldehyde N-(2,6-dimethylphenyl) semicarbazone (EGA) was synthesized as in [47 (link)].