GGTase-I was obtained from Jena Bioscience (Jena, Germany) and D*-GCVLL (dansyl gly-cys-val-leu-leu) from Calbiochem (Darmstadt, Germany). Ammonium hydroxide solution 28–30% was purchased from Alfa Aesar (Karlsruhe, Germany), the phosphatase inhibitors Halt® and Phosstop® from Thermo-Fisher/Piercenet (Bonn, Germany) and Roche Diagnostics GmbH (Mannheim, Germany) and the GGTase-I inhibitor GGTI-2133 from Sigma Aldrich (Schnelldorf, Germany). All solvents were of analytical grade or higher quality. Acetonitrile was obtained from Carl Roth GmbH (Karlsruhe, Germany), 1-butanol, n-hexane, 2-propanol, methanol, acetone, ammonium acetate and assay buffer compounds: Tris–HCl, MgCl2, ZnCl2 and Na2CO3 were obtained from Merck (Darmstadt, Germany). GGPP, octyl- -Dglucopyranoside and dithiothreitol were from Sigma-Aldrich (Schnelldorf, Germany). Millipore water was used for all solutions (Schwalbach, Germany).
Ammonium hydroxide solution 28 30
Manufactured by Thermo Fisher Scientific
Sourced in Germany
Ammonium hydroxide solution 28–30% is a concentrated aqueous solution of ammonia. It is a colorless, pungent liquid with a distinct ammonia odor. The solution is typically used as a chemical reagent in various laboratory applications.
Automatically generated - may contain errors
Lab products found in correlation
Mgcl2, by Merck Group (2 mentions)
Zncl2, by Merck Group (2 mentions)
Tris hcl, by Merck Group (2 mentions)
Dithiothreitol (dtt), by Merck Group (2 mentions)
Methanol, by Merck Group (2 mentions)
Ammonium acetate, by Merck Group (2 mentions)
N hexane, by Merck Group (2 mentions)
Acetone, by Merck Group (2 mentions)
2 propanol, by Merck Group (2 mentions)
Na2co3, by Merck Group (2 mentions)
Acetonitrile, by Carl Roth (2 mentions)
1 butanol, by Merck Group (2 mentions)
Ggti 2133, by Merck Group (1 mentions)
Ggtase 1, by Jena Biosciences (1 mentions)
7 8 dihydroxyflavone hydrate, by Merck Group (1 mentions)
Octyl β d glucopyranoside, by Merck Group (1 mentions)
2 protocols using ammonium hydroxide solution 28 30
1
Geranylgeranyl Transferase-I Inhibitor Assay
2
7,8-Dihydroxyflavone Hydrate Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
7,8-Dihydroxyflavone hydrate (#D5446; purity > 98%; CAS Number 38183-03-8)) was purchased from Sigma-Aldrich (Darmstadt, Germany); ammonium hydroxide solution 28–30% was purchased from Alfa Aesar (Karlsruhe, Germany); and the phosphatase inhibitors Halt® and Phosstop® from Thermo-Fisher/Piercenet (Bonn, Germany) and Roche Diagnostics GmbH (Mannheim, Germany). All solvents were of analytical grade or higher quality. Acetonitrile was obtained from Carl Roth GmbH (Karlsruhe, Germany), 1-butanol, n-hexane, 2-propanol, methanol, acetone, ammonium acetate and assay buffer compounds: Tris–HCl, MgCl2, ZnCl2 and Na2CO3 were obtained from Merck (Darmstadt, Germany). Octyl-β-d-glucopyranoside and dithiothreitol were from Sigma-Aldrich (Schnelldorf, Germany). Millipore water was used for all solutions (Schwalbach, Germany).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!