Rosa26 confetti

Manufactured by Jackson ImmunoResearch

The Rosa26-Confetti is a genetic reporter system that allows for the visualization of cell lineage and fate in mouse models. It is a tool used in biomedical research to study cell differentiation and development.

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5 protocols using rosa26 confetti

Genetically Engineered Mouse Lines

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All animal procedures were performed in accordance with the protocol approved by the Institutional Animal Care and Use Committee at Stanford University. Six to 10-week-old mice on a C57BL/6J background purchased from The Jackson Laboratory, Bar Harbor, ME, were utilized for all studies: wild-type C57BL/6J mice (JAX#00664), GFAP-Cre (JAX#12886), ChAT-Cre (JAX#6410), Rosa26 eGFP (JAX#4077), Rosa26 Confetti (JAX#13731), and Rosa26 TDT (JAX#7914).

Suarez-Mier G.B, & Buckwalter M.S. (2015). Glial Fibrillary Acidic Protein-Expressing Glia in the Mouse Lung. ASN NEURO, 7(5), 1759091415601636.

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Genetically Modified Mouse Strains

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Wild-type C57BL/6J (CD45.2), Rosa26^{Lox-Stop-Lox-tdTomato} (AI1433) and Rosa26^{Confetti 34} mice were purchased from The Jackson Laboratory (strain numbers: 000664, 007914 and 013731, respectively). Strains that were kindly provided to us: Aicda^{CreERT2 21} by J.-C. Weill and C.-A. Reynaud (Université Paris-Descartes), S1pr2^CreERT2 BAC-transgenic ^{25 (link)} by T. Kurosaki and T. Okada (U. Osaka, RIKEN-Yokohama), CD20-CreERT2^{27 (link)} by M. Shlomchik (U. Pittsburgh) and Prdm1^{flox/flox 35 (link)} by J. Boss (Emory University). OT-II TCR transgenic (Y-chromosome)^{36 (link)} CD45.1 mice were bred and maintained in our laboratory. All mice were held at the immunocore clean facility at the Rockefeller University under specific pathogen-free conditions. All mouse procedures were approved by the Rockefeller University’s Institutional Animal Care and Use Committee.

Schiepers A., van ‘t Wout M.F., Hobbs A., Mesin L, & Victora G.D. (2023). Opposing effects of pre-existing antibody and memory T cell help on the dynamics of recall germinal centers. bioRxiv.

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Characterization of Genetically Engineered Mice

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Floxed Osterix (Osx^fl/fl) mice^{28 (link)} were a gift from B. de Crombrugghe (University of Texas MD Anderson Cancer Center). PthrP-mCherry mice^{2 (link)} were a gift from Noriaki Ono (UT Health School of Dentistry). Rosa26-tTA mice²⁹ were a gift from Luigi Puglielli (University of Wisconsin-Madison). Rosa26^confetti (Stock 017492), NOD scid gamma (NSG, Stock 005557), NSG-EGFP (Stock 021937), Rosa26^mT/mG (Stock 007676), R26-M2rtTA (Stock 006965), pTRE-H2BGFP (Stock 005104), Prrx1-cre (Stock 005584), Ocn-cre (Stock 019509), Pax7-cre (Stock 010530), Shh-cre (Stock 005622), Mpz-cre (Stock 017927), MIP-GFP (Ins1-EGFP, Stock #: 006864), floxed Stat3 (Stat3^fl/fl) mice (Stock 016923) and UBC-GFP (Stock 004353) mice were purchased from Jackson Laboratories. Mfge8^−/− (RBRC01726) mice^{30 (link)} were purchased from RIKEN bioresource research center. Shn3 loxp mice^{31 (link)} were reported previously. All mice were maintained on a C57BL6/J background throughout the study except for experiments involving 4T1.2 cells that were conducted in BALB/cJ mice. All animals were maintained in accordance with the NIH Guide for the Care and Use of Laboratory Animals and were handled according to protocols approved by the Weill Cornell Medical College institutional animal care and use committee (IACUC).

Sun J., Hu L., Bok S., Yallowitz A.R., Cung M., McCormick J., Zheng L.J., Debnath S., Niu Y., Tan A.Y., Lalani S., Morse K.W., Shinn D., Pajak A., Li Z., Li N., Xu R., Iyer S, & Greenblatt M.B. (2023). Discovery of a Vertebral Skeletal Stem Cell Driving Spinal Metastases. Research Square.

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Rosa26 Confetti and Cre ER Mouse Breeding

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Rosa26-Confetti and Rosa26-Cre^ER were purchased from the Jackson Laboratories and bred at Emory University. All animals were housed in an Emory University Department of Animal Resources facility (Atlanta, GA) and used in accordance with an Institutional Animal Care and Use Committee–approved protocol.

Martinez R.J., Neeld D.K, & Evavold B.D. (2015). Identification of T cell clones without the need for sequencing. Journal of immunological methods, 424, 28-31.

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Genetically Modified Mice for Confetti Lineage Tracing

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PF4-Cre^{24 (link)} and Rosa26-Confetti^{23 (link)} were purchased from The Jackson Laboratory and maintained and cross-bred at our animal facility (stock no: 008535 and 017492). vWF-Cre mice were a gift of W. Aird.^{25 (link)} Mhy9 fl/fl mice were a gift from Dr. Gachet.^{26 (link)} All strains were backcrossed to and maintained on C57BL/6J-background. If not otherwise stated, animals of same sex and age were randomly assigned to experimental groups, and PF4-Cre negative control animals were used. We used Rosa26^Confetti/+ animals expressing Cre for Online Supplementary Figure S1. The other experiments were performed with homozygous Rosa26^{Confetti/Confetti} mice expressing Cre. Cre negative animals were used as controls. All procedures performed on mice were approved by the local legislation on the protection of animals (Regierung von Oberbayern, Munich).

Nicolai L., Kaiser R., Escaig R., Hoffknecht M.L., Anjum A., Leunig A., Pircher J., Ehrlich A., Lorenz M., Ishikawa-Ankerhold H., Aird W.C., Massberg S, & Gaertner F. (2021). Single platelet and megakaryocyte morpho-dynamics uncovered by multicolor reporter mouse strains in vitro and in vivo. Haematologica, 107(7), 1669-1680.

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