Seeds from 67 samples were planted and grown under standardized greenhouse conditions for tissue collection. The fourth set of true leaves from each plant was collected and total RNA extracted using NucleoSpin RNA Plant kits (Macherey‐Nagel) following manufacturer's instructions. RNA‐seq libraries were prepared using the Illumina TruSeq Stranded mRNA Library Prep Kit (Illumina, Inc.), with library quality subsequently analyzed by qPCR using KAPA Library Quantification kits (Kapa Biosystems, Inc.), Qubit 2.0 Fluorometric (Invitrogen, Life Technologies), and a 2100 Bioanalyzer. Sample libraries were pooled according to the sequencing instrument's requirements and sequenced using Illumina HiSeq2500.
Qubit 2.0 fluorometric
Manufactured by Thermo Fisher Scientific
The Qubit 2.0 Fluorometer is a compact and accurate instrument designed for measuring the concentration of DNA, RNA, and protein samples. It utilizes fluorescence-based detection technology to provide precise quantification of sample concentrations.
Automatically generated - may contain errors
Lab products found in correlation
Nanodrop 1000, by Thermo Fisher Scientific (2 mentions)
Bioanalyzer 2100, by Thermo Fisher Scientific (1 mentions)
Kapa library quantification kit, by Roche (1 mentions)
Hiseq 2500, by Illumina (1 mentions)
Truseq stranded mrna library prep kit, by Illumina (1 mentions)
Nucleospin rna plant kit, by Macherey-Nagel (1 mentions)
Zr fungal bacterial dna miniprep kit, by Zymo Research (1 mentions)
2 protocols using qubit 2.0 fluorometric
1
Transcriptome Analysis of Plant Leaves
2
Bacterial DNA Isolation and Sequencing
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ZR Fungal/Bacterial DNA Mini-Prep Kit (Zymo Research, Irvine, CA) was used to obtain high-quality bacterial DNA. DNA quantification was performed using Nanodrop 1000 (Thermo Fisher Scientific) and Qubit 2.0 Fluorometric (Invitrogen; Thermo Fisher Scientific). Paired-end Illumina sequencing libraries were prepared with Illumina Nextera XT sample preparation kit (Illumina, Inc., San Diego, CA) in accordance with the manufacturer’s recommendations and were sequenced on in-house Illumina Miseq (Illumina) platform with 2 × 250 bp paired-end sequencing kit.
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