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Mpdca1

Manufactured by Miltenyi Biotec
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Sourced in United States

The MPDCA1 is a multi-purpose digital cell analyzer designed for high-throughput cell analysis. It is capable of performing various cell-based assays, including flow cytometry, cell counting, and cell viability assessment. The MPDCA1 provides accurate and reliable data, supporting researchers in their scientific investigations.

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Lab products found in correlation

Apc conjugated streptavidin, by BD (1 mentions) Facs fortessa, by BD (1 mentions) Accuri, by BD (1 mentions) Ack lysis buffer, by Thermo Fisher Scientific (1 mentions) Collagenase a, by Merck Group (1 mentions) Facscanto flow cytometer, by BD (1 mentions) Fix perm buffer set, by BioLegend (1 mentions) T bet, by BD (1 mentions)

3 protocols using mpdca1

1

Multiparametric Flow Cytometry Immunophenotyping

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After saturation with anti-CD32/CD16, cells were incubated with mAbs reactive to mPDCA1 (JF05-1C2.4.1, conjugated to FITC), MHCII (IA/IE, 2G9 or M5/114.15.2, FITC or PE), CD103 (M290 or 145-2D11, PE), CD86 (GL1, FITC), CD11b (M1/70, PerCP Cy5.5) or CD11c (HL3, Biotin or BV786). For T-cell staining, cells were incubated with mAbs reactive to CD4 (L3T4 or RM4-5, FITC or PECy7), CD45.2 (104, A780), CD3 (145-2C11, PerCP Cy5.5) or CD8 (Ly2, 53-6.8, Biotin or APC). For B-cell staining, cells were incubated with mAbs reactive to CD19 (1D3, APC), B220 (RA36B2, APC), CD45.2 (104, A780), CD5 (53-7.3, BV421) or CD23 (B3B4, FITC). All mAbs were purchased from BD Biosciences (San Jose, CA, USA) except for mPDCA1 (Miltenyi Biotec, Paris, France). APC-conjugated streptavidin (BD Biosciences) was used to label biotin Abs. At least 2 × 106 events were acquired using an Accuri or Fortessa FACS (BD Biosciences) and analyzed using FlowJo Sofware v7.5 (Tree Star Inc., Ashland, OR, USA).
Remot A., Descamps D., Noordine M.L., Boukadiri A., Mathieu E., Robert V., Riffault S., Lambrecht B., Langella P., Hammad H, & Thomas M. (2017). Bacteria isolated from lung modulate asthma susceptibility in mice. The ISME Journal, 11(5), 1061-1074.
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2

Flow Cytometry Analysis of Immune Cells

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For flow cytometry analysis, lungs were collected at different time points (days 3, 5, 7 and 9 post-infection) after rhMPV (-WT or -ΔG) or mock infection and digested with collagenase A (0.5 mg/ml PBS; Sigma-Aldrich, St. Louis, MO). Single cell suspension was prepared by passing the cells through nylon mesh, and contaminating erythrocytes were lysed using ACK lysis buffer (Invitrogen). Live gated cells were incubated with Fc block (anti-mouse CD16/CD32) for 30 min to reduce nonspecific binding before addition of Abs (BD Pharmingen, San Diego, CA) against surface markers: anti-CD3ε, -CD4, -CD8β (T cells); -CD19, B220 (B cells); DX5, NK1.1 (NK cells); -CD11c, -CD11b, MHCII, F4/80, Ly6G & Ly6C (Gr-1), and -mPDCA1 (Miltenyi Biotec) (cDCs, pDCs, macrophages, and neutrophils). Relevant isotype control antibodies were used throughout. Samples were stained and run through a FACS Canto flow cytometer (BD Pharmingen, San Diego, CA) and data was analyzed using the FlowJo Software (Tree Star).
Velayutham T.S., Ivanciuc T., Garofalo R.P, & Casola A. (2022). Role of human metapneumovirus glycoprotein G in modulation of immune responses. Frontiers in Immunology, 13, 962925.
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3

Multiparametric Analysis of Splenic Immune Cells

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Spleens from 10-mo-old mice were freshly isolated and minced extensively. Red blood cells were lysed using hypertonic solution (BioLegend, San Diego, CA, USA). Live splenic leukocytes were stained with fluorochrome-labeled monoclonal Abs (moAbs) recognizing CD4, CD40, CD86, and CD11c (BD Biosciences, San Jose, CA, USA), PD-1, CXCR5 (eBioscience, San Diego, CA, USA), F4/80, I-AB, and B220 (BioLegend, San Diego, CA, USA), mPDCA1 (Miltenyi Biotec, Germany). For intracellular staining, splenic leukocytes were fixed and permeabilized using a proprietary Fix/Perm buffer set (BioLegend) and stained with moAbs recognizing FoxP3 (BioLegend) and T-bet (BD Biosciences). Flow cytometry data were acquired with FACScan Aria and Canto machines (BD Biosciences) and analyzed with FlowJo software (TreeStar, Ashland, OR, USA).
Wong A.H., Agrawal N, & Hughes G.C. (2015). Altered IgG autoantibody levels and CD4+ T cell subsets in lupus-prone Nba2 mice lacking the nuclear progesterone receptor. Autoimmunity, 48(6), 389-401.
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